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家蚕幼虫中肠细菌群落多样性的PCR-DGGE和16S rDNA文库序列分析 被引量:57
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作者 相辉 李木旺 +3 位作者 赵勇 赵立平 张月华 黄勇平 《昆虫学报》 CAS CSCD 北大核心 2007年第3期222-233,共12页
采用基于16S rDNA的变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)和16S rDNA文库序列分析的手段,研究了重要经济昆虫家蚕Bombyxmori2个品系——专食性品系C108和广食性品系SCN2幼虫中肠内的细菌群落多样性,同时还... 采用基于16S rDNA的变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)和16S rDNA文库序列分析的手段,研究了重要经济昆虫家蚕Bombyxmori2个品系——专食性品系C108和广食性品系SCN2幼虫中肠内的细菌群落多样性,同时还探讨了食料对家蚕中肠内细菌群落结构的影响。文库序列分析表明,PCR扩增得到的16S rDNA基因代表了家蚕中肠内的41种细菌系统发育型(phylotype),大多数属于Proteobacteria,其次是Lactobacillales。此外,还有少数属于Deinococcus-Thermus、Bacillales、Clostridiales和Actinobacteria,尚有5种系统发育型不能确定其所属类型。家蚕的这2个品系中,肠球菌属Enterococcus是其中肠细菌的优势菌群,栖热菌属Thermus是次优势菌群。优势菌肠球菌属的组成在品系和不同食料喂养条件下有着一定的变化,无桑饲料喂养条件下SCN2品系中肠内还出现了新的次优势菌葡萄球菌(Staphylococcus)。DGGE图谱显示家蚕低龄幼虫和高龄幼虫肠道细菌格局存在差异,推测可能与其发育期生理状态的差异有关。本研究结果提示家蚕肠道特殊菌群的出现可能与其特殊的食性有一定的关系,食料改变、生长受阻后肠道微生态平衡也发生变化。 展开更多
关键词 家蚕 专食性 广食性 中肠细菌 变性梯度凝胶电泳 16S rDNA文库分析
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氯虫苯甲酰胺胁迫下二化螟中肠细菌类微生物的多样性 被引量:1
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作者 张珏锋 张琴 +2 位作者 李芳 钟海英 陈建明 《中国水稻科学》 CAS CSCD 北大核心 2020年第6期586-594,共9页
【目的】明确氯虫苯甲酰胺处理下二化螟中肠细菌类微生物多样性的变化。【方法】综合运用宏基因组测序以及传统微生物分离纯化的方法分析不同浓度氯虫苯甲酰胺处理的二化螟种群中肠微生物的多样性。【结果】不同浓度(100、200、400μg/m... 【目的】明确氯虫苯甲酰胺处理下二化螟中肠细菌类微生物多样性的变化。【方法】综合运用宏基因组测序以及传统微生物分离纯化的方法分析不同浓度氯虫苯甲酰胺处理的二化螟种群中肠微生物的多样性。【结果】不同浓度(100、200、400μg/m L)氯虫苯甲酰胺处理后二化螟中肠细菌类微生物丰度及多样性降低,处理种群中肠细菌类微生物的OTU数值、特有的OTU数值均低于对照;摩根氏菌属(Morganella)、普罗威登斯菌属(Providencia)以及变形菌属(Proteus)在处理种群中的比例明显升高;同时培养结果显示氯虫苯甲酰胺处理种群获得的菌株除肠杆菌属(Enterobacter)外,还存在超压莱略特氏菌(Lelliottia nimipressuralis)、非脱羧勒菌(Leclercia adecarboxylata)、成团泛菌(Pantoea agglomerans)等其他菌属。COG数据库对比与KEGG分析显示,对照与处理二化螟种群的各功能分类基因数量及相对丰度无明显差异。【结论】解析了氯虫苯甲酰胺胁迫下二化螟中肠细菌类微生物的多样性。这些结果为进一步研究中肠细菌在二化螟对氯虫苯甲酰胺的抗药性奠定基础。 展开更多
关键词 二化螟 氯虫苯甲酰胺 宏基因组 中肠细菌 微生物多样性
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取食四种寄主植物对番茄潜叶蛾中肠肠道细菌的影响
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作者 杨亚贤 周昭旭 +3 位作者 钱秀娟 刘月英 张美娇 蒋明君 《中国生物防治学报》 CSCD 北大核心 2024年第4期776-786,共11页
番茄潜叶蛾Tuta absoluta是一种世界性害虫,寄主范围广泛,对多种寄主作物造成严重危害。通过16S rRNA测序分析了取食番茄、马铃薯、茄子、和人参果的番茄潜叶蛾2龄幼虫中肠肠道细菌的多样性和功能。结果显示,在上述四种寄主植物为食的... 番茄潜叶蛾Tuta absoluta是一种世界性害虫,寄主范围广泛,对多种寄主作物造成严重危害。通过16S rRNA测序分析了取食番茄、马铃薯、茄子、和人参果的番茄潜叶蛾2龄幼虫中肠肠道细菌的多样性和功能。结果显示,在上述四种寄主植物为食的番茄潜叶蛾中肠肠道细菌中,共检测到4886、6016、2781、3471个操作分类单元(OTU),其中共有905个OUT。Alpha和Beta多样性分析结果表明,取食马铃薯和茄子的番茄潜叶蛾中肠肠道细菌多样性最高。物种分类分析表明,取食不同寄主的番茄潜叶蛾,中肠肠道细菌在门水平的优势菌群均为厚壁菌门和变形菌门;在属水平上,取食番茄和马铃薯的番茄潜叶蛾中肠肠道细菌优势属为沃尔巴克氏体,取食人参果和茄子的为肠球菌属;对其代谢通路分析,发现取食四种寄主植物其中肠肠道细菌代谢通路相似,无显著差异,且占比最高的代谢通路都为生物合成和降解代谢。 展开更多
关键词 番茄潜叶蛾 不同寄主植物 细菌 菌群多样性
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三种革兰阴性细菌在按蚊饲养水体及按蚊中肠内存活能力的研究
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作者 李美 汤林华 《国际医学寄生虫病杂志》 CAS 2010年第3期148-151,共4页
目的 探讨分离自中华按蚊雌成蚊中肠的3种革兰阴性细菌,即气单孢菌、A4菌(未鉴定)和肠杆菌在按蚊幼虫孳牛水体中及按蚊体内的存活能力. 方法 3种目标细菌在实验室经数量扩增后,添加到饲养按蚊幼虫的水体中,依次收集饲养盆和蛹盒内的... 目的 探讨分离自中华按蚊雌成蚊中肠的3种革兰阴性细菌,即气单孢菌、A4菌(未鉴定)和肠杆菌在按蚊幼虫孳牛水体中及按蚊体内的存活能力. 方法 3种目标细菌在实验室经数量扩增后,添加到饲养按蚊幼虫的水体中,依次收集饲养盆和蛹盒内的水样品,并解剖羽化后吸血雌成蚊,取其中肠制成研磨液;建立水样品和研磨液中细菌的16S rDNA序列克隆文库,并从各样品中分离可培养细菌,比较各样品中的优势菌种和不同处理中可培养细菌的种类组成. 结果 在所有样品中均未检测到与加入的人工培养细菌种类相同的细菌.按蚊幼虫饲养水体和蛹盒水体中的可培养细菌均以嗜水气单孢菌和睾丸酮丛毛单胞菌为主,在少量按蚊的中肠内含有可培养细菌,但均不是目标细菌;而各处理样品中的优势菌均为铜绿假单孢菌. 结论 3种细菌经人工培养后,不能在按蚊幼虫生活水体中建立种群,导致未能在按蚊成蚊中肠内柃测到这些细菌. 展开更多
关键词 细菌菌群 优势细菌 中肠细菌 施用 经发育期传递
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Etiology of inflammatory bowel disease:A unified hypothesis 被引量:14
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作者 Xiaofa Qin 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第15期1708-1722,共15页
Inflammatory bowel disease(IBD),including both ulcerative colitis(UC) and Crohn's disease(CD),emerged and dramatically increased for about a century.Despite extensive research,its cause remains regarded as unknown... Inflammatory bowel disease(IBD),including both ulcerative colitis(UC) and Crohn's disease(CD),emerged and dramatically increased for about a century.Despite extensive research,its cause remains regarded as unknown.About a decade ago,a series of findings made me suspect that saccharin may be a key causative factor for IBD,through its inhibition on gut bacteria and the resultant impaired inactivation of digestive proteases and over digestion of the mucus layer and gut barrier(the Bacteria-Protease-Mucus-Barrier hypothesis).It explained many puzzles in IBD such as its emergence and temporal changes in last century.Recently I further found evidence suggesting sucralose may be also linked to IBD through a similar mechanism as saccharin and have contributed to the recent worldwide increase of IBD.This new hypothesis suggests that UC and CD are just two symptoms of the same morbidity,rather than two different diseases.They are both caused by a weakening in gut barrier and only differ in that UC is mainly due to increased infiltration of gut bacteria and the resultant recruitment of neutrophils and formation of crypt abscess,while CD is mainly due to increased infiltration of antigens and particles from gut lumen and the resultant recruitment of macrophages and formation of granulomas.It explained the delayed appearance but accelerated increase of CD over UC and many other phenomena.This paper aims to provide a detailed description of a unified hypothesis regardingthe etiology of IBD,including the cause and mechanism of IBD,as well as the relationship between UC and CD. 展开更多
关键词 ETIOLOGY Inflammatory bowel disease Ulcerative colitis Crohn's disease Dietary chemicals SACCHARIN SUCRALOSE
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Neutron (Magnetic Isotope) Catalysis for Example Isotopes 24,25,26Mg in Cells E. Coli
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作者 Aibassov Yerkin Zhakenovich Yemelyanova Valentina Tussupbayev Nessipba~ Shakieva Tatyana Yerzhanova Zhadyra 《Journal of Chemistry and Chemical Engineering》 2015年第1期71-74,共4页
We offered the new theory of neutron (magnetic isotope) catalysis. For the first time it was shown that the number of neutrons in the atom, which have anomalous magnetic effect, have a great influence on the chemica... We offered the new theory of neutron (magnetic isotope) catalysis. For the first time it was shown that the number of neutrons in the atom, which have anomalous magnetic effect, have a great influence on the chemical properties. Our proposed theory of neutron (magnetic isotope) catalysis takes into account the influence of the magnetic field on the catalytic processes. 展开更多
关键词 Neutron (magnetic isotope) catalysis magnetic field catalytic processes.
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Indication of Bacteria in Water Marshes (Southern Iraq)
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作者 Wathiq Abaas Hatit Reyam Naji Ajmi +1 位作者 Abdal-Kader Saeed Latif Abdul-Jabbar Jasim Jryan 《Journal of Environmental Science and Engineering(A)》 2013年第6期353-358,共6页
The study was conducted in water marshes for five major marshes during the months of January, April and September of each region (Hawizeh, Hammer and the central marsh) in southern Iraq. Bacteriological analysis tha... The study was conducted in water marshes for five major marshes during the months of January, April and September of each region (Hawizeh, Hammer and the central marsh) in southern Iraq. Bacteriological analysis that some of the default pathological bacteria, including TC (Total bacterial count), FCB (faecal coliform bacteria), MPN (coliform bacteria) and FS (faecal streptococcus) can evidence to indicate of pollution. The results showed that most of the water samples examined pathological contain bacteria which indicates that the water is unfit for human consumption and the waters as basal (more hydrogen than No. 7). There was a high salinity more than the permissible limits for human consumption and even to irrigate crops, and the same case for dissolve oxygen which exceeded are other limits as drinking water. 展开更多
关键词 Water contamination physical properties BACTERIA Iraqi marshland.
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