Study and Application of Fingerprints of Ginkgo biloba Leaves Preparations

Aim To establish a method for determination of Ginkgo biloba L, its extractand preparations with HPLC fingerprints, so as to control the quality of the preparations. MethodsHPLC-DAD method was used to determine the constituents in preparations. Diamonsil? C_(18) (200mm X 4.6 mm, 5 μm) was used as analytical column, and acetonitrile/KH_2PO_4 was used as mobilephase with gradient elu-tion. The column temperature was at 24 ℃. The HPLC profile of chemicalconstituents of control sample and preparations were analyzed using similarity software. Results Thefingerprints of different preparations from different companies were slightly different because ofthe different preparing procedures. Mean while, the fingerprints of different batches of the samepreparation from the same company were similar to each other and the technology of each preparationwas stable. Conclusion This method is accurate, reproducible , simple, and can be used as ananalytical method for the routine quality control of Ginkgo biloba preparations.

HPLC测定复方祛癣颗粒中盐酸巴马汀、盐酸小檗碱的含量

目的建立同时测定复方祛癣颗粒中盐酸巴马汀、盐酸小檗碱含量的方法。方法采用Shim-pack ODS(250×4.6mm,5μm)色谱柱,流动相为乙睛(A)-0.3%磷酸水溶液(三乙胺调节p H值至3.0)(B),梯度洗脱(0~22 min,22%A;22~24 min,22%~24%A;24~40 min,22%A),流速1.0 m L·min-1,检测波长为345 nm,柱温40℃。结果盐酸巴马汀、盐酸小檗碱进样量分别在25.044~75.12μg·m L-1(r=0.999 4)、22.64~226.4μg·m L-1(r=0.999 6)与峰面积呈良好线性关系,平均回收率(n=6)分别为99.77%、99.11%,且RSD分别为0.78%和0.69%。精密度RSD均小于1.56%,重复性RSD均小于1.70%,供试品溶液放置12h内稳定。结论本方法准确、简便,重复性好。可用于复方祛癣颗粒多指标质量评价及控制提供科学依据。

杀菌止痒洗剂的HPLC指纹图谱研究

目的:建立杀菌止痒洗剂的HPLC指纹图谱分析方法。方法:采用Comatex C18-AA(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.04%三乙胺缓冲液(磷酸调pH至6.5)梯度洗脱,流速1 mL.min-1,检测波长220 nm,柱温30℃。采用《中药色谱指纹图谱相似度评价系统(2004A版)》计算相似度。结果:根据10批杀菌止痒洗剂的对照指纹图谱,确定了15个共有峰,其相似度均大于0.9。结论:该方法简便、稳定、重复性好,可为杀菌止痒洗剂质量控制提供依据。

HPLC-DAD法同时测定清开灵注射液中7个酚酸类成分

目的:建立同时测定清开灵注射液中7个酚酸类成分(新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸)含量的方法。方法:采用Agilent Zorbax SB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为0.1%甲酸水溶液(A)-乙腈(B),梯度洗脱,流速为0.5 mL·min-1,检测波长327 nm,柱温30℃。结果:80min内7个待测组分分离度良好,在各自的检测范围内与峰面积呈良好的线性,其相关系数均大于0.9998,加样回收率为96.1%～103.9%。应用所建立的方法同时测定了清开灵注射液中新绿原酸、绿原酸、隐绿原酸、咖啡酸、4,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸共7个成分的含量,并对5个不同批次的注射液进行了含量测定,其结果有一定的差异。结论:本法可为清开灵注射液中酚酸类成分的质量控制提供参考。

HPLC法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量

目的:建立反相高效液相色谱法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量。方法:采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.5%磷酸(38∶62)为流动相,流速1.0 mL.min-1,检测波长210 nm,柱温30℃。结果:绞股蓝皂苷B进样量在0.04～2.0μg范围内,与峰面积呈良好线性关系;平均回收率(n=6)为102.1%,RSD为1.2%。结论:该方法简便、准确,重复性好,可作为绞股蓝总苷胶囊的质量控制方法。