[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided...[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided into four groups, and were fed with forage supplemented with 0 （control group）, 0.2%, 0.4% and 0.6% Zengrujianniusan for 60 d. During this period, the contents of fat, protein and non-fat solid in milk were measured every 20 d. [Result] The milk production of the cows whose forage was added with 0.2 %, 0.4 % and 0.6% Zengrujianniusan was 4.02%, 12.50%, 14.00% higher than that of the control （P〉0.05）. The herbal feed additive had no significant influence on the contents of fat, milk and non-fat solid in milk, but significantly reduced the number of somatic cells. [Conclusion] The study will provide reference for developing feed additives which is safe and non-toxic to cows and their milk.展开更多
Four Holstein dairy cows in the 7th month of late lactation were sampled, and self-control method was used. Daidzein was added to the concentrated feed at the dose of 10mg/kg during the treatment stage. Results showed...Four Holstein dairy cows in the 7th month of late lactation were sampled, and self-control method was used. Daidzein was added to the concentrated feed at the dose of 10mg/kg during the treatment stage. Results showed that daidzein lessened the declining tendency of milk yield in late lactation and increased the milk yield to some extents. Lactoprotein was evidently increased (P<0.01), while milkfat was not affected evidently. The level of T4 in serum was evidently increased (P<0.05); the levels of GH, PRL, E2 and TSH in serum were prone to rising while T3 was prone to decreasing . It was concluded that daidzein could indirectly affect milk yield and components of milk by regulating the levels of intrinsic hormones.展开更多
The plant secondary metabolites （PSM） are highly sought compounds for use as an alternative to conventionally used feed additives in animal production these days; Siamese neem leaf （Azadirachta indica A. Juss. var....The plant secondary metabolites （PSM） are highly sought compounds for use as an alternative to conventionally used feed additives in animal production these days; Siamese neem leaf （Azadirachta indica A. Juss. var. siamenses Valeton） and Zanthoxylum pods （Zanthoxylum piperatum） are known to contain numerous such compounds.The objectives of this study were to determine effects of feeding Siamese neem leaf and Zanthoxylum pods as feed additives on dry matter intake （DMI）, dry matter digestibility （DMD） and milk production and milk composition. Lactating Thai Holstein cows （n = 8） were arranged in two replicates of 4 x 4 Latin square designs, housed in individual stall, treatments consisted of Siamese neem 0.5 and 1.5 g kgl dry matter （DM）, ZanthoxylumO. 1 and 0.5 g kg1 DM added to the total mixed ration （TMR）. TMRand drinking water were provided ad lib. There were no significant differences （P 〉 0.05） in DMI and crude protein （CP）, acid detergent fiber （ADF）, neutral detergent fiber （NDF） digestibility, milk production and composition among the treatments.However, significant difference （P 〈 0.05） was observed in dry matter （DM） and ether extract （EE） digestibility. Though statistically non-significant, Zanthoxylum at higher dose level tended to show low DMI （14.85 kg） in cows, while promotingmarginally higher milk （14.18 kg） production as compared with lower dose levelwhere DMI and milk production were 16.14 kg and 13.83 kg.This indicated that Zanthoxylum has a potential to improve feed digestion in dairy cows when used as additives.展开更多
The performance of some growth and milk traits of selected Qomi native （Lori black） goats and hybrids of saanen with native goats during 2008-2010 was compared. Growth traits include birth weight （BW）, one-month w...The performance of some growth and milk traits of selected Qomi native （Lori black） goats and hybrids of saanen with native goats during 2008-2010 was compared. Growth traits include birth weight （BW）, one-month weight （1 MW）, weaning weight （WW） and 6-month weight （6 MW）. Also the milk production traits was monthly milk production on 2, 3, 4, 5th months （quantitative traits） and some milk composition included dry matter （%DM）, fat （%F）, and protein （%P） percentage on during of production （qualitative traits）. There were no significant differences in sex, birth type and genetic group for BW. Also the effect of genetic groups on 1 MW, WW, and 6 MW was significant, while effects of sex and birth type on 1 MW, WW and 6 MW was not significant. Also MW （Mothe＇s weight） had no significant effect on BW, 1 MW, WW and 6 MW. The average of daily milk records was 0.48 kg for nativel and 1.1 kg for crossed goats. The maximum monthly milk records in two groups were in second month （native： 0.64 -4- 0.05, crossed： 1.44 ~： 0.12） and the minimum were in fifth month （native： 0.36 ＋ 0.03, crossed： 0.77 -4- 0.05）. %DM, %F and %P in 5th month were higher than other months in two groups. Effects of genetic groups on milk production and also on average of%F, %DM and %P were significant. This study showed that the crossbreeding has a significant effect on most milk production and growth traits in Iranian native goats.展开更多
In Brazil, milk production is a key sector for income generation, tax collection and much of the milk produced comes from family farms. The present study was aimed to assess the conditions of milk production on 30 far...In Brazil, milk production is a key sector for income generation, tax collection and much of the milk produced comes from family farms. The present study was aimed to assess the conditions of milk production on 30 farms in the city of S~ Mateus, to the North of the state of Espirito Santo, Brazil. Data were obtained through questionnaires sent to producers according to the legislation of the Brazilian Ministry of Agriculture. The questions included type of milking, hygiene practices and health status of milkers, mastitis control and source and others. Milking was manually done in all farms, and only 40,0% of milkers washed their hands and forearms before milking, and 13.0% used antiseptic solutions after washing. None of the farms performed the pre-dipping and post-dipping procedures. Control of mastitis was not performed in 94.0% of the farms and none of them performed the CMT test. A high degree of non-conformity was observed in the family farms. These data demonstrate the need for training farmers in good agricultural practices. This would contribute to achieving better quality milk, and ensure the sustainability of the segment in the Northern region of the state of Espirito Santo, Brazil.展开更多
One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were conf...One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were confirmed positive for the presence of this pathogen. Stapylococcus aureus isolates identified appeared typically and atypically characteristics of growth colonies in Baird Parker agar plates at 37°C for 24-48 h. 6 out of 19 strains showed typical characteristics and 13 out 19 （68%） isolates had atypical characteristics. Coagulase positive Staphylococcus aureus was found in 18% （1911 05） of fresh milk samples. Milk produced by cows with subclinical mastitis largely influences Staphylococcus aureus count of bulk tank milk than contamination of milking and milk handling equipment by this pathogen. All S. aureus isolates were coagulase positive. 5 （or about 25%） of isolates of S. aureus identified were resistant to penicillin, 7 out 19 isolates or 36% resistant to methicilin and 9 out 19 strains or 47% resistant to vancomycin. Another objective of this study was the evaluation of the number of S. aureus per mL raw milk. The results confirmed that II （57.8%） out of 19 positive cases had more than 100 cfu/mL, indicating a possible risk for intoxication caused by production of entertoxines of coagulase positive S. aureus.展开更多
The main purpose of this study was to detect an association of cytoplasmic signal transducers and activators of transcription-1 （STAT1） with milk production traits in 472 Holstein and 283 Jersey cattle breeds of Tur...The main purpose of this study was to detect an association of cytoplasmic signal transducers and activators of transcription-1 （STAT1） with milk production traits in 472 Holstein and 283 Jersey cattle breeds of Turkey. This gene, located on chromosome 2, was chosen due to its role on development of mammary gland. A polymorphism of selected 314 bp allele fragment was detected by the restriction fragment length polymorphism analysis of polymerase chain reaction-amplified fragments （PCR-RFLP） method and also confirmed by DNA sequencing. The association tests were conducted between STAT1 genotypes and some economically important dairy traits. The genotypes for C/T as a single nucleotide polymorphism （SNP） were identified at interval 60 cM to 63 cM. The effects of STAT1 gene on milk production traits were not significant in Holstein cows, although animals with CT genotypes showed fairly close to significant value for the corrected 305 d milk yield. However, Jersey cows with/7＂ genotype were 2.07 kg higher for test-day milk yield （P 〈 0.05）, 0.13 kg for fat yield （P 〈 0.01） and 0.07 kg for protein yield （P 〈 0.05） compared with animals having CC and CT genotypes. Definitely, the further research should be conducted to search this gene intensively with larger samples to identify polymorphism and any association between the economically important traits and genotypic class in Holstein cows. Finally, based on the findings, it was concluded that STATI gene might be used as a potential candidate gene to improve milk yield and milk fat and protein contents in dairy cows breeding programs.展开更多
The present study was conducted in four districts of Telangana State of India with 120 respondents in which 60 were members and other 60 were non-members of DCSs （Dairy Co-operative Societies）/MPIs （Milk Producer I...The present study was conducted in four districts of Telangana State of India with 120 respondents in which 60 were members and other 60 were non-members of DCSs （Dairy Co-operative Societies）/MPIs （Milk Producer Institutions）. Constraints perceived in dairy farming and suggestions elicited by members and non-members were noted, tabulated and analyzed. The major constraints perceived by half or more than half of members were scarcity of water （91.67%）, lack of green fodder （88.33%）, preferring NS （natural service） than AI （Artificial Insemination） （83.33%）, negligence in feeding pregnant and dry animals （81.37%）, insufficient loan amount （78.33%）, tendency to feed only grass and brans （71.67%）, lack of availability of sufficient labour （70.00%）, lack of remunerative price for milk （66.67%）, high cost of feed （61.67%）, lack of fodder conservation （55.00%） and high cost of animals （50.00%）. Whereas major constraints perceived by non-members were scarcity of water （96.67%）, lack of green fodder （93.33%）, preferring NS than AI （88.33%）, lack of availability of sufficient labour （85.00%）, negligence in feeding pregnant and dry animals （83.33%）, tendency to feed only grass and brans （78.33%）, high cost of animals （75.00%）, complex procedure for obtaining loans （71.67%） and high cost of feed （66.67%）. Suggestions elicited by members majorly were providing subsidy for purchase of animals/providing loans on par with the cost of animal （81.67%）, enhancing the procurement price （70.00%）, providing more incentives （60.00%）, encouraging calf rearing （58.33%） and conducting frequent animal health camps （50.00%）. In case of non-members major suggestions elicited were conducting frequent animal health camps （68.33%） and providing subsidy on purchase of animals/providing loans on par with the cost of animal （58.33%）. Considering the above constraints perceived and suggestions elicited by dairy farmers a proper linkage strategy should be developed among private and public sectors in providing need based services to the dairy farmers.展开更多
The epidemiology of Salmonella involves food, especially meat and dairy products. These have a high salmonella outbreak in the summer. The majority of Salmonella enteritis in young children occurs in the form of scatt...The epidemiology of Salmonella involves food, especially meat and dairy products. These have a high salmonella outbreak in the summer. The majority of Salmonella enteritis in young children occurs in the form of scattered cases. At least 25% of summer gastroenteritis in young children is caused by Salmonella. In Western Europe, S. typhimurium accounts for nearly 70% of isolates. The objective of this study was to investigate the Salmonella contaminating meat and dairy products as well as define the percentages of contamination by Salmonella from 2004 to 2006, by the Department of Hygiene of Tunis. For this study, we collected samples in various motherboard solutions, and conducted a pre-nonselective enrichment in selective enrichment in isolation and identification and ultimately biochemical confirmation. One hundred and sixty two samples, 125 samples of meat products including 68 samples of red meat （beef and sheep, beef, offal and Merguez） and 57 samples of poultry （chicken and turkey）, 37 samples of milk products which included 32 samples of cheese and five samples milk, were analysed microbiologically from 2004 to 2006, in the Health Service in Tunis. These analyses include the detection and enumeration of Salmonella. From 2004 to 2006, the rate of infections by the Salmonella, meat and dairy products were： 55.9% for red meat, 71.9% for poultry, 68.8% for cheeses, 40% for milk. The meat of poultry is contaminated with Salmonella that the cheeses, which are more than red meats, which are also more contaminated than milk. This might be due to a lack of hygiene throughout the circuit processing （slaughter, transport, processing, etc.）. The increased risk of contamination of milk products by Salmonella is proportional to the manipulation done on these products. The latter must be handled by pasteurization and sterilization.展开更多
Probiotics administration can improve host health. This study aims to determine the effects of probiotics （Lactobacillus casei Zhang and Lactobacillus plantarum P-8） administration on milk production, milk func- tio...Probiotics administration can improve host health. This study aims to determine the effects of probiotics （Lactobacillus casei Zhang and Lactobacillus plantarum P-8） administration on milk production, milk func- tional components, milk composition, and fecal microbiota of dairy cows. Variations in the fecal bacteria microbiota between treatments were assessed based on 16S rRNA profiles determined by PacBio single molecule real-time sequencing technology. The probiotics supplementation significantly increased the milk production and the contents of milk immunoglobulin C （IgG）, lactoferrin （LTF）, lysozyme （LYS） and lactoperoxidase （LP）, while the somatic cell counts （SCC） significantly decreased （P〈0.01）. However, no significant difference was found in the milk fat, protein and lactose contents （P 〉 0.05）. Although the probiotics supplementation did not change the fecal bacteria richness and diversity, significantly more rumen fermentative bacteria （ Bacteroides, Roseburia, Ruminococcus, CIostridium, Coprococcus and Dorea） and beneficial bacteria （Faecalibacterium prausnitzii） were found in the probiotics treatment group. Meanwhile, some opportunistic pathogens e.g. Bacillus cereus, Cronobacter sakazakii and Alkaliphilus oremlandii, were suppressed. Additionally, we found some correlations between the milk production, milk components and fecal bacteria. To sum up, our study demonstrated the beneficial effects of probiotics application in improving the quality and quantity of cow milk production.展开更多
基金Supported by Science and Technology Development Program of Shijiazhuang City(08150132A)China Spark Program(2012GA6200025)~~
文摘[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided into four groups, and were fed with forage supplemented with 0 （control group）, 0.2%, 0.4% and 0.6% Zengrujianniusan for 60 d. During this period, the contents of fat, protein and non-fat solid in milk were measured every 20 d. [Result] The milk production of the cows whose forage was added with 0.2 %, 0.4 % and 0.6% Zengrujianniusan was 4.02%, 12.50%, 14.00% higher than that of the control （P〉0.05）. The herbal feed additive had no significant influence on the contents of fat, milk and non-fat solid in milk, but significantly reduced the number of somatic cells. [Conclusion] The study will provide reference for developing feed additives which is safe and non-toxic to cows and their milk.
基金Elitist Innovation Fund of Henan Province (12100030)
文摘Four Holstein dairy cows in the 7th month of late lactation were sampled, and self-control method was used. Daidzein was added to the concentrated feed at the dose of 10mg/kg during the treatment stage. Results showed that daidzein lessened the declining tendency of milk yield in late lactation and increased the milk yield to some extents. Lactoprotein was evidently increased (P<0.01), while milkfat was not affected evidently. The level of T4 in serum was evidently increased (P<0.05); the levels of GH, PRL, E2 and TSH in serum were prone to rising while T3 was prone to decreasing . It was concluded that daidzein could indirectly affect milk yield and components of milk by regulating the levels of intrinsic hormones.
文摘The plant secondary metabolites （PSM） are highly sought compounds for use as an alternative to conventionally used feed additives in animal production these days; Siamese neem leaf （Azadirachta indica A. Juss. var. siamenses Valeton） and Zanthoxylum pods （Zanthoxylum piperatum） are known to contain numerous such compounds.The objectives of this study were to determine effects of feeding Siamese neem leaf and Zanthoxylum pods as feed additives on dry matter intake （DMI）, dry matter digestibility （DMD） and milk production and milk composition. Lactating Thai Holstein cows （n = 8） were arranged in two replicates of 4 x 4 Latin square designs, housed in individual stall, treatments consisted of Siamese neem 0.5 and 1.5 g kgl dry matter （DM）, ZanthoxylumO. 1 and 0.5 g kg1 DM added to the total mixed ration （TMR）. TMRand drinking water were provided ad lib. There were no significant differences （P 〉 0.05） in DMI and crude protein （CP）, acid detergent fiber （ADF）, neutral detergent fiber （NDF） digestibility, milk production and composition among the treatments.However, significant difference （P 〈 0.05） was observed in dry matter （DM） and ether extract （EE） digestibility. Though statistically non-significant, Zanthoxylum at higher dose level tended to show low DMI （14.85 kg） in cows, while promotingmarginally higher milk （14.18 kg） production as compared with lower dose levelwhere DMI and milk production were 16.14 kg and 13.83 kg.This indicated that Zanthoxylum has a potential to improve feed digestion in dairy cows when used as additives.
文摘The performance of some growth and milk traits of selected Qomi native （Lori black） goats and hybrids of saanen with native goats during 2008-2010 was compared. Growth traits include birth weight （BW）, one-month weight （1 MW）, weaning weight （WW） and 6-month weight （6 MW）. Also the milk production traits was monthly milk production on 2, 3, 4, 5th months （quantitative traits） and some milk composition included dry matter （%DM）, fat （%F）, and protein （%P） percentage on during of production （qualitative traits）. There were no significant differences in sex, birth type and genetic group for BW. Also the effect of genetic groups on 1 MW, WW, and 6 MW was significant, while effects of sex and birth type on 1 MW, WW and 6 MW was not significant. Also MW （Mothe＇s weight） had no significant effect on BW, 1 MW, WW and 6 MW. The average of daily milk records was 0.48 kg for nativel and 1.1 kg for crossed goats. The maximum monthly milk records in two groups were in second month （native： 0.64 -4- 0.05, crossed： 1.44 ~： 0.12） and the minimum were in fifth month （native： 0.36 ＋ 0.03, crossed： 0.77 -4- 0.05）. %DM, %F and %P in 5th month were higher than other months in two groups. Effects of genetic groups on milk production and also on average of%F, %DM and %P were significant. This study showed that the crossbreeding has a significant effect on most milk production and growth traits in Iranian native goats.
文摘In Brazil, milk production is a key sector for income generation, tax collection and much of the milk produced comes from family farms. The present study was aimed to assess the conditions of milk production on 30 farms in the city of S~ Mateus, to the North of the state of Espirito Santo, Brazil. Data were obtained through questionnaires sent to producers according to the legislation of the Brazilian Ministry of Agriculture. The questions included type of milking, hygiene practices and health status of milkers, mastitis control and source and others. Milking was manually done in all farms, and only 40,0% of milkers washed their hands and forearms before milking, and 13.0% used antiseptic solutions after washing. None of the farms performed the pre-dipping and post-dipping procedures. Control of mastitis was not performed in 94.0% of the farms and none of them performed the CMT test. A high degree of non-conformity was observed in the family farms. These data demonstrate the need for training farmers in good agricultural practices. This would contribute to achieving better quality milk, and ensure the sustainability of the segment in the Northern region of the state of Espirito Santo, Brazil.
文摘One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were confirmed positive for the presence of this pathogen. Stapylococcus aureus isolates identified appeared typically and atypically characteristics of growth colonies in Baird Parker agar plates at 37°C for 24-48 h. 6 out of 19 strains showed typical characteristics and 13 out 19 （68%） isolates had atypical characteristics. Coagulase positive Staphylococcus aureus was found in 18% （1911 05） of fresh milk samples. Milk produced by cows with subclinical mastitis largely influences Staphylococcus aureus count of bulk tank milk than contamination of milking and milk handling equipment by this pathogen. All S. aureus isolates were coagulase positive. 5 （or about 25%） of isolates of S. aureus identified were resistant to penicillin, 7 out 19 isolates or 36% resistant to methicilin and 9 out 19 strains or 47% resistant to vancomycin. Another objective of this study was the evaluation of the number of S. aureus per mL raw milk. The results confirmed that II （57.8%） out of 19 positive cases had more than 100 cfu/mL, indicating a possible risk for intoxication caused by production of entertoxines of coagulase positive S. aureus.
文摘The main purpose of this study was to detect an association of cytoplasmic signal transducers and activators of transcription-1 （STAT1） with milk production traits in 472 Holstein and 283 Jersey cattle breeds of Turkey. This gene, located on chromosome 2, was chosen due to its role on development of mammary gland. A polymorphism of selected 314 bp allele fragment was detected by the restriction fragment length polymorphism analysis of polymerase chain reaction-amplified fragments （PCR-RFLP） method and also confirmed by DNA sequencing. The association tests were conducted between STAT1 genotypes and some economically important dairy traits. The genotypes for C/T as a single nucleotide polymorphism （SNP） were identified at interval 60 cM to 63 cM. The effects of STAT1 gene on milk production traits were not significant in Holstein cows, although animals with CT genotypes showed fairly close to significant value for the corrected 305 d milk yield. However, Jersey cows with/7＂ genotype were 2.07 kg higher for test-day milk yield （P 〈 0.05）, 0.13 kg for fat yield （P 〈 0.01） and 0.07 kg for protein yield （P 〈 0.05） compared with animals having CC and CT genotypes. Definitely, the further research should be conducted to search this gene intensively with larger samples to identify polymorphism and any association between the economically important traits and genotypic class in Holstein cows. Finally, based on the findings, it was concluded that STATI gene might be used as a potential candidate gene to improve milk yield and milk fat and protein contents in dairy cows breeding programs.
文摘The present study was conducted in four districts of Telangana State of India with 120 respondents in which 60 were members and other 60 were non-members of DCSs （Dairy Co-operative Societies）/MPIs （Milk Producer Institutions）. Constraints perceived in dairy farming and suggestions elicited by members and non-members were noted, tabulated and analyzed. The major constraints perceived by half or more than half of members were scarcity of water （91.67%）, lack of green fodder （88.33%）, preferring NS （natural service） than AI （Artificial Insemination） （83.33%）, negligence in feeding pregnant and dry animals （81.37%）, insufficient loan amount （78.33%）, tendency to feed only grass and brans （71.67%）, lack of availability of sufficient labour （70.00%）, lack of remunerative price for milk （66.67%）, high cost of feed （61.67%）, lack of fodder conservation （55.00%） and high cost of animals （50.00%）. Whereas major constraints perceived by non-members were scarcity of water （96.67%）, lack of green fodder （93.33%）, preferring NS than AI （88.33%）, lack of availability of sufficient labour （85.00%）, negligence in feeding pregnant and dry animals （83.33%）, tendency to feed only grass and brans （78.33%）, high cost of animals （75.00%）, complex procedure for obtaining loans （71.67%） and high cost of feed （66.67%）. Suggestions elicited by members majorly were providing subsidy for purchase of animals/providing loans on par with the cost of animal （81.67%）, enhancing the procurement price （70.00%）, providing more incentives （60.00%）, encouraging calf rearing （58.33%） and conducting frequent animal health camps （50.00%）. In case of non-members major suggestions elicited were conducting frequent animal health camps （68.33%） and providing subsidy on purchase of animals/providing loans on par with the cost of animal （58.33%）. Considering the above constraints perceived and suggestions elicited by dairy farmers a proper linkage strategy should be developed among private and public sectors in providing need based services to the dairy farmers.
文摘The epidemiology of Salmonella involves food, especially meat and dairy products. These have a high salmonella outbreak in the summer. The majority of Salmonella enteritis in young children occurs in the form of scattered cases. At least 25% of summer gastroenteritis in young children is caused by Salmonella. In Western Europe, S. typhimurium accounts for nearly 70% of isolates. The objective of this study was to investigate the Salmonella contaminating meat and dairy products as well as define the percentages of contamination by Salmonella from 2004 to 2006, by the Department of Hygiene of Tunis. For this study, we collected samples in various motherboard solutions, and conducted a pre-nonselective enrichment in selective enrichment in isolation and identification and ultimately biochemical confirmation. One hundred and sixty two samples, 125 samples of meat products including 68 samples of red meat （beef and sheep, beef, offal and Merguez） and 57 samples of poultry （chicken and turkey）, 37 samples of milk products which included 32 samples of cheese and five samples milk, were analysed microbiologically from 2004 to 2006, in the Health Service in Tunis. These analyses include the detection and enumeration of Salmonella. From 2004 to 2006, the rate of infections by the Salmonella, meat and dairy products were： 55.9% for red meat, 71.9% for poultry, 68.8% for cheeses, 40% for milk. The meat of poultry is contaminated with Salmonella that the cheeses, which are more than red meats, which are also more contaminated than milk. This might be due to a lack of hygiene throughout the circuit processing （slaughter, transport, processing, etc.）. The increased risk of contamination of milk products by Salmonella is proportional to the manipulation done on these products. The latter must be handled by pasteurization and sterilization.
基金supported by the China Agriculture Research System(CARS-37)the Major Project of the Inner Mongolia Autonomous region
文摘Probiotics administration can improve host health. This study aims to determine the effects of probiotics （Lactobacillus casei Zhang and Lactobacillus plantarum P-8） administration on milk production, milk func- tional components, milk composition, and fecal microbiota of dairy cows. Variations in the fecal bacteria microbiota between treatments were assessed based on 16S rRNA profiles determined by PacBio single molecule real-time sequencing technology. The probiotics supplementation significantly increased the milk production and the contents of milk immunoglobulin C （IgG）, lactoferrin （LTF）, lysozyme （LYS） and lactoperoxidase （LP）, while the somatic cell counts （SCC） significantly decreased （P〈0.01）. However, no significant difference was found in the milk fat, protein and lactose contents （P 〉 0.05）. Although the probiotics supplementation did not change the fecal bacteria richness and diversity, significantly more rumen fermentative bacteria （ Bacteroides, Roseburia, Ruminococcus, CIostridium, Coprococcus and Dorea） and beneficial bacteria （Faecalibacterium prausnitzii） were found in the probiotics treatment group. Meanwhile, some opportunistic pathogens e.g. Bacillus cereus, Cronobacter sakazakii and Alkaliphilus oremlandii, were suppressed. Additionally, we found some correlations between the milk production, milk components and fecal bacteria. To sum up, our study demonstrated the beneficial effects of probiotics application in improving the quality and quantity of cow milk production.
