西汉上层社会中的“免冠、徒跣,稽颡”等请罪礼

"免冠、徒跣,稽颡请罪"与"免冠、徒跣,稽首谢"都是大臣向皇帝请罪的完整仪式,也可省称免冠谢、徒跣谢、免冠徒跣谢、稽颡谢和免冠稽首谢等;"免冠、徒跣,顿首谢"本是大臣之间请罪的完整仪式,也可省称免冠谢、免冠顿首谢、顿首谢和叩头谢等,由于顿首与稽颡、稽首在请罪时只是头触地的时间长短略有差别,便不再细分,顿首也可用于大臣向皇帝的请罪;"去簪珥,徒跣顿首谢"是公主向皇帝请罪的完整仪式,省称脱簪请罪、脱簪珥叩头等。这些请罪礼广泛应用于西汉上层社会,对缓和君臣以及大臣之间的关系起了应有的作用。

“加冠”与“免冠”

冠词的用法是初中英语重点语法项目之一。不少同学总感到冠词难用,尤其拿不准什么时候要“加冠”,什么时候要“免冠”。本文着重介绍“定冠词的用法”和“不用冠词的几种情况”的两则顺口溜,大家不妨一试。一、定冠词的用法特指双熟悉,上文已提及;世上独无二,序数最高级;方位专有名,习语及乐器。

一步步教你做数码免冠照

相信许多朋友都有这样的经历：填写应聘表、考个驾照或是办个工作证经常会用到免冠照，可每次都想不起来上回留下的底片放在哪里。最后只好一边抱怨自己记性不好一边走进照相馆，一番讨价还价之后，然后极不情愿地把银子数给照相馆老板，到了下回这样的情形依然重现。其实，只要我们做好一个无需底片的数码免冠照存放在个人电脑里，

Immunogenicity of the Spike Glycoprotein of Bat SARS-like Coronavirus

A group of SARS-like coronaviruses(SL-CoV)have been identified in horseshoe bats.Despite SL-CoVs and SARS-CoV share identical genome structure and high-level sequence similarity,SL-CoV does not bind to the same cellular receptor as for SARS-CoV and the N-terminus of the S proteins only share 64%amino acid identity,suggesting there are fundamental differences between these two groups of coronaviruses.To gain insight into the basis of this difference,we established a recombinant adenovirus system expressing the S protein from SL-CoV(rAd-Rp3-S)to investigate its immune characterization.Our results showed that immunized mice generated strong humoral immune responses against the SL-CoV S protein.Moreover,a strong cellular immune response demonstrated by elevated IFN-γand IL-6 levels was also observed in these mice.However,the induced antibody from these mice had weaker cross-reaction with the SARS-CoV S protein,and did not neutralize HIV pseudotyped with SARS-CoV S protein.These results demonstrated that the immunogenicity of the SL-CoV S protein is distinct from that of SARS-CoV,which may cause the immunological differences between human SARS-CoV and bat SL-CoV.Furthermore,the recombinant virus could serve as a potential vaccine candidate against bat SL-CoV infection.

Identification of Immunogenic Determinants of the Spike Protein of SARS-like Coronavirus

Bat SARS-Iike coronavirus （SL-CoV） has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike （S） proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have been reports studying the overall immunogenicity of SsL, knowledge on the precise location of immunodominant determinants for SSL is still lacking. In this study, using a series of truncated expressed SsL fragments and SsL specific mouse sera, we identified two immunogenic determinants for SSL. Importantly, one of the two regions seems to be located in a region not shared by known immunogenic determinants of the SSARS. This finding will be of potential use in future monitoring of SL-CoV infection in bats and spillover animals and in development of more effective vaccine to cover broad protection against this new group of coronaviruses.

Free triiodothyronine in relation to coronary severity at different ages： Gensini score assessment in 4206 euthyroid patients

Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 euthyroid patients were consecutively enrolled and di- vided into CAD group （n = 3306） and non-CAD group （n = 900）. All patients underwent coronary angiography （CAG）. Gensini score （GS） was used to determine the severity of coronary artery stenosis. Severe CAD was defined as GS 〉 32 and mild CAD was defined as GS 〈 32. Logistic regression analysis and linear regression analysis were conducted to determine the association of FT3 with CAD in patients with different gender and ages. Results Concentration of FT3 was lower in patients with CAD than that in angiography-normal control group （P 〈 0.05）. In addition, concentration of FT3 was lower in severe CAD than that in mild CAD. After adjusting for traditional cardiovascular risk factors and potential confounders, FT3 was negatively correlated with the presence of CAD, but not in the old patients （〉 65 years old）. Mul- tivariable linear regression analysis showed that FT3 was negatively associated with GS in male and young patients with stable CAD, but not in the old patients. Conclusions Low FT3 within normal range was negatively associated with the presence and severity of CAD in young patients, but not in the old ones. Further studies are needed to confirm our findings.

PIKA Provides an Adjuvant Effect to Induce Strong Mucosal and Systemic Humoral Immunity Against SARS-CoV

Severe Acute Respiratory Syndrome （SARS） is a deadly infectious disease caused by SARS Coronavirus （SARS-CoV）. Inactivated SARS-CoV has been explored as a vaccine against SARS-CoV. However, safe and potent adjuvants, especially with more efficient and economical needle-free vaccination are alw needed more urgently in a pandemic. The development of a safe and effective mucosal adjuvant and vaccine ays for prevention of emergent infectious diseases such as SARS will be an important advancement. PIKA, a stabilized derivative of Poly （I：C）, was previously reported to be safe and potent as adjuvant in mouse models. In the present study, we demonstrated that the intraperitoneal and intranasal co-administration of inactivated SARS-CoV vaccine together with this improved Poly （I：C） derivative induced strong anti-SARS-CoV mucosal and systemic humoral immune responses with neutralizing activity against pseudotyped virus. Although intraperitoneal immunization of inactivated SARS-CoV vaccine alone could induce a certain level of neutralizing activity in serum as well as in mucosal sites, co-administration of inactivated SARS-CoV vaccine with PIKA as adjuvant could induce a much higher neutralizing activity. When intranasal immunization was used, PIKA was obligatorily for inducing neutralizing activity in serum as well as in mucosal sites and was correlated with both mucosal IgA and mucosal IgG response. Overall, PIKA could be a good mucosal adjuvant candidate for inactivated SARS-CoV vaccine for use in possible future pandemic.

Canopy Carbon Exchange on a Coarse-Sand-Field, No-tillage Peach Orchard

Plant carbon sequestration is an effective way to abate the global warming. However, the field-scale carbon exchange on a peach orchard remains unclear. Here, using an eddy covariance technique, the net ecosystem carbon dioxide exchange and energy balance were analyzed on a coarse-sand-field, no-tillage, 12-year-old-peach orchard. The results showed that during full flowering, the ability to sequestrate carbon was significant, it reached on the peak of-0.33 mg （CO2） m^-2 s^-1. During rapid growth, the Bowen ratio was under 0.3 and daily net carbon sequestration reached on the peak of-25.1 g （CO2） m^-2 d^-1. During the leaf fall stage, there is a great deal of CO2 emissions, the peak value of carbon sequestration reached ＋0.60 mg （CO2） m^-2 s^-1. During monitoring period, the daily average of net carbon sequestration and Bowen ratio was 1.22 ± 1.56 and -2.90 ± 6.63 g （CO2） m^-2 d^-1, respectively. The net carbon sequestration could reach -1,052 g （CO2） m^-2 in a year. These results reveal that there is high carbon sequestration on a coarse-sand-field, no-tillage peach orchard.

参加“新农保”时需要提供哪些资料?

答:农村居民首次参保时需提供以下资料:户口簿、身份证件及复印件1份(第二代居民身份证,未换证的由公安部门出具第二代居民身份证证明)、1寸近期免冠照片2张。

A novel human coronavirus:Middle East respiratory syndrome human coronavirus

In 2012,a novel coronavirus,initially named as human coronavirus EMC(HCoV-EMC) but recently renamed as Middle East respiratory syndrome human coronavirus(MERS-CoV),was identified in patients who suffered severe acute respiratory infection and subsequent renal failure that resulted in death.Ongoing epidemiological investigations together with retrospective studies have found 61 laboratory-confirmed cases of infection with this novel coronavirus,including 34 deaths to date.This novel coronavirus is culturable and two complete genome sequences are now available.Furthermore,molecular detection and indirect immunofluorescence assay have been developed.The present paper summarises the limited recent advances of this novel human coronavirus,including its discovery,genomic characterisation and detection.

Association between SNP rs1800875,serum chymase and immunoglobulin E levels in patients with coronary heart disease

Objective:The gene for mast cell chymase (CMA1) is an ideal candidate for investigating the genetic predisposition to coronary heart disease (CHD),as activated mast cells have been found to be present in a greater proportion in the shoulder region of atheroma than in normal coronary intimae.Previous studies have indicated that CMA1 promoter polymorphism rs1800875 may be involved in regulating immunoglobulin E (IgE) levels in patients with eczema,and it is associated with the progression of immunoglobulin A nephropathy.Methods:The association between single nucleotide polymorphism (SNP) rs1800875,serum chymase,and serum IgE levels was examined in 175 CHD subjects and 95 non-CHD subjects.Results:Statistical analysis indicated no significant difference in allele frequency between CHD and non-CHD.However,a significant association was found between CMA1 genotypes and total IgE levels in CHD subjects.Meanwhile,crossover analysis revealed that,in GG homozygotes,CHD risk was nearly six times higher in those with IgE (U/ml) level <2.58 (natural logarithm conversion),while no association was found with chymase level.Conclusions:Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in CHD subjects,but not with chymase level in both groups.In GG homozygotes,high IgE level is a protective factor against coronary disease.

Essential sequence of the N-terminal cytoplasmic localization-related domain of huntingtin and its effect on huntingtin aggregates

Huntington＇s disease （HD） is caused by abnormal CAG repeat expansion in the 5＇-end of the Huntingtin （HTT） gene. In addition to the canonical C-terminal full-length huntingtin （htt） nuclear export signal, a cytoplasmic localization-related domain （CLRD） in the N-terminus of htt has recently been reported. Here, we analyzed this domain by introducing deletion and substitution mutations in a truncated N-terminal htt protein and subsequently monitored htt expression, aggregation and subcellular localization by immunocytochemistry and Western blot analysis. We demonstrated that Htt1-17 was the essential sequence for htt cytoplasmic localization. We also found that the subcellular distribution of htt was altered when Htt1_17 was mutated to contain amino acids of different charges, suggesting a structural requirement of Htt1-17 for the cytoplasmic localization of htt. Deletion of the first three amino acids did not affect its association with mitochondria. We observed that defective cytoplasmic localization resulted in a reduction of total htt aggregates and increased nuclear aggregates, indicating that the subcellular distribution of the protein might influence the aggregation process. These studies provide new insight into the molecular mechanism of htt aggregation in HD.