AIM. To assess the inhibitory effect of HuangqiZhechong decoction on hepatic fibrosis in rats induced by CCl4 plus alcohol and high fat low protein diet.METHODS: Male SD rats were randomly divided into hepati cfibrosi...AIM. To assess the inhibitory effect of HuangqiZhechong decoction on hepatic fibrosis in rats induced by CCl4 plus alcohol and high fat low protein diet.METHODS: Male SD rats were randomly divided into hepati cfibrosis model group, control group and 3 treatment groups consisting of 12 rats in each group. Except for the normal control group, all the rats were subcutaneously injected with CCI4 at a dosage of 3 mL/kg. In 3 treated groups, eithe rhigh-dose group (9 mL/kg), or medium-dose group (6 mL/kg),or low-dose group (3 mL/kg) was daily garaged with Huangqi Zhechong decoction, and saline vehicle was given to model and normal control rats. Enzyme-linked immunosorbent assay (ELISA) and biochemical examinations were used to determine the changes of alanine aminotransferase (ALT),aspartate aminotransferase (AST), hyaluronic acid (HA),laminin (LN), type-III-procollagen-N-peptide (PIIIP), and type IV collagen content in serum, and hydroxyproline (Hyp) content in liver after sacrificing the rats. Pathologic changes,particularly fibrosis were examined by hematoxylin and eosin (HE) and Van Gieson staining.RESULTS: Compared with the model control group; serum ALT, AST, HA, LN, PIIIP and type IV collagen levels dropped markedly in Huangqi Zhechong decoction groups, especially in the medium-dose Huangqi Zhechong decoction group (1 954±576 U/L vs 759±380 U/L, 2 735±786 U/L vs 1259±829 U/L, 42.74±7.04 ng/mL vs20.68±5.85 ng/mL,31.62±5.84 ng/mL vs 14.87±1.45 ng/mL, 3.26±0.69 ng/mL vs 1.47±0.46 ng/mL, 77.68±20.23 ng/mL vs 25.64±4.68 ng/mL, respectively) (P<0.05). The Hyp content in liver tissue was also markedly decreased (26.47+11.24 mg/mgprot vs 9.89±3.74 mg/mgprot) (P<0.01). Moreover, the stage ofthe rat liver fibrosis in Huangqi Zhechong decoction groups was lower than that in model group, and more dramatic drop was observed in medium-dose Huangqi Zhechong decoction group (P<O,01),CONCLUSION: Huangqi Zhechong decoction can inhibit hepatic fibrosis resulted from chronic liver injure, retard the development of cirrhosis, and notably ameliorate the liver function. It may be a safe and effective therapeutic drug for patients with fibrosis.展开更多
Dot-Enzyme-linked Immunosorbent Assay (Dot-ELISA) was employed for the detection of serum ant/sporm antibodies (AsAb). We found that in 140 cases of infertile
The antifertility effect of epididymal injection of Sodium Morrhuate ( SM ) via the vas deferens as a new contraceptive method for males is definitely positive. However, the mechanism of its action has not been well u...The antifertility effect of epididymal injection of Sodium Morrhuate ( SM ) via the vas deferens as a new contraceptive method for males is definitely positive. However, the mechanism of its action has not been well understood. Further research in this field展开更多
Objective: To detect the serum granulocyte colony-stimulating factor (G-CSF) levels between the patients with frequently repeated infection (repeaters) and others (non-repeaters) in different phase of infection. Metho...Objective: To detect the serum granulocyte colony-stimulating factor (G-CSF) levels between the patients with frequently repeated infection (repeaters) and others (non-repeaters) in different phase of infection. Methods: An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF levels in 50 cases (32 non-repeaters and 18 repeaters) with acute phase of infection. Serum G-CSF levels were detected in recovery phase in 10 cases. Results: Serum G-CSF levels were significantly higher (1429.97±506.43ng/L) in 32 non-repeaters with acute infection. There was a positive correlation between white blood cell count (WBC) and serum G-CSF level (r =0. 396, P <0.05). There was also a positive correlation between absolute neutrophil count (ANC) and serum G-CSF level (r=0.346,P<0,05). Serum G-CSF levels were higher (98.62 -+56.40ng/L) in 18 repeaters with acute infection, It was showed that serum G-CSF levels were significantly higher in non-repeaters than in repeaters with acute phase of infection ( P <0. 001 ). In the meanwhile, the body temperature was significantly higher in non-repeaters than in repeaters with acute infection (37.95±0.14℃ rs 36.91±0.13℃ ,P <0.001), There were no significant differences in age, WBC, ANC, type of bacterial, liver function and renal function (P > 0.05). Serum G-CSF levels in recovery phase of the two groups were below the sensitivity of the assay ( < 60 ng/L), Conlusion: It is suggested that application of recombinant G-CSF may be useful for the patients with repeated infection,展开更多
AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expres...AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expressed as a GST-fusion protein, and interaction of HCV E2 protein with a tupaia CD81 LEL was evaluated by enzyme-linked immunosorbent assay (EIA). RESULTS: Although tupaia and human CD81 LEL differed in 6 amino acid changes, tupaia CD81 LEL was strongly recognized by anti-CD81 antibodies against human CD81 LEL conformation-dependent epitopes. Investigating LEL CD81-E2 interactions by EIA, we demonstrated that binding of tupaia CD81 LEL GST fusion protein to recombinant HCV E2 protein was markedly reduced compared to binding of human CD81 LEL GST fusion protein to recombinant HCV E2 protein. CONCLUSION: These data suggest that the structural differences in-between the tupaia and human CD81 may alter the interaction of the large extracellular loop with HCV envelope glycoprotein E2. These findings may be important for the understanding of the mechanisms of binding and entry of HCV to PTHs.展开更多
AIM： To investigate possibility and clinical application of fecal calprotectin in determining disease activity of ulcerative colitis （UC）. METHODS： The enzyme-linked immunosorbent assay （ELISA） was used to measu...AIM： To investigate possibility and clinical application of fecal calprotectin in determining disease activity of ulcerative colitis （UC）. METHODS： The enzyme-linked immunosorbent assay （ELISA） was used to measure the concentrations of calprotectin in feces obtained from 66 patients with UC and 20 controls. C-reactive protein （CRP）, erythrocyte sedimentation rate （ESR）, acid glycoprotein （AGP） were also measured and were compared with calprotectin in determining disease activity of UC. The disease activity of UC was also determined by the Sutherland criteria. RESULTS： The fecal calprotectin concentration in the patients with active UC was significantly higher than that in the inactive UC and in the controls （402.16 ± 48.0 μg/g vs 35.93 ± 3.39 μg/g, 11.5 ± 3.42 μg/g, P 〈 0.01）. The fecal calprotectin concentration in the inactive UC group was significantly higher than that in the control group （P 〈 0.05）. A significant difference was also found in the patients with active UC of mild, moderate and severe degrees. The area under the curve of the receiver operating characteristics （AUCR^c） was 0.975, 0.740, 0.692 and 0.737 for fecal calprotectin, CRP, ESR and AGP, respectively. There was a strong correlation between the fecal calprotectin concentration and the endoscopic gradings for UC （r = 0.866, P 〈 0.001）. CONCLUSION： Calprotectin in the patient＇s feces can reflect the disease activity of UC and can be used as a rational fecal marker for intestinal inflammation in clinical practice. This kind of marker is relatively precise, simple and noninvasive when compared with other commonlyused markers such as CRP, ESR and AGP.展开更多
AIM:To assess Helicobacter pylori(Hpylon)seroprevalence in a cohort of Greek patients with lung cancer. METHODS:Seventy-two lung cancer patients(55 males and 17 females,aged 58.2±11.7 years)and 68,age and gender-...AIM:To assess Helicobacter pylori(Hpylon)seroprevalence in a cohort of Greek patients with lung cancer. METHODS:Seventy-two lung cancer patients(55 males and 17 females,aged 58.2±11.7 years)and 68,age and gender-matched,control subjects were enrolled.All subjects underwent an enzyme-linked immunosorbent assay IgG serologic test for Hpylori diagnosis. RESULTS:A correlation between age and HpyloriIgG level was detected for both lung cancer patients(r=0.42, P=0.004)and controls(r=0.44,P=0.004).Seropositivity for Hpyloridid not differ significantly between patients with lung cancer and controls(61.1% vs 55.9%,P>0.05). Concerning the mean serum concentration of IgG antibodies against Hpylori,no significant difference between the two groups was detected(32.6±19.1 vs 27.4±18.3 U/mL, P>0.05). CONCLUSION:No significant association between Hpylori infection and lung cancer was found.展开更多
基金Supported by theScience and Technology Foundation of Shaanxi Province,No.2002k11-G7
文摘AIM. To assess the inhibitory effect of HuangqiZhechong decoction on hepatic fibrosis in rats induced by CCl4 plus alcohol and high fat low protein diet.METHODS: Male SD rats were randomly divided into hepati cfibrosis model group, control group and 3 treatment groups consisting of 12 rats in each group. Except for the normal control group, all the rats were subcutaneously injected with CCI4 at a dosage of 3 mL/kg. In 3 treated groups, eithe rhigh-dose group (9 mL/kg), or medium-dose group (6 mL/kg),or low-dose group (3 mL/kg) was daily garaged with Huangqi Zhechong decoction, and saline vehicle was given to model and normal control rats. Enzyme-linked immunosorbent assay (ELISA) and biochemical examinations were used to determine the changes of alanine aminotransferase (ALT),aspartate aminotransferase (AST), hyaluronic acid (HA),laminin (LN), type-III-procollagen-N-peptide (PIIIP), and type IV collagen content in serum, and hydroxyproline (Hyp) content in liver after sacrificing the rats. Pathologic changes,particularly fibrosis were examined by hematoxylin and eosin (HE) and Van Gieson staining.RESULTS: Compared with the model control group; serum ALT, AST, HA, LN, PIIIP and type IV collagen levels dropped markedly in Huangqi Zhechong decoction groups, especially in the medium-dose Huangqi Zhechong decoction group (1 954±576 U/L vs 759±380 U/L, 2 735±786 U/L vs 1259±829 U/L, 42.74±7.04 ng/mL vs20.68±5.85 ng/mL,31.62±5.84 ng/mL vs 14.87±1.45 ng/mL, 3.26±0.69 ng/mL vs 1.47±0.46 ng/mL, 77.68±20.23 ng/mL vs 25.64±4.68 ng/mL, respectively) (P<0.05). The Hyp content in liver tissue was also markedly decreased (26.47+11.24 mg/mgprot vs 9.89±3.74 mg/mgprot) (P<0.01). Moreover, the stage ofthe rat liver fibrosis in Huangqi Zhechong decoction groups was lower than that in model group, and more dramatic drop was observed in medium-dose Huangqi Zhechong decoction group (P<O,01),CONCLUSION: Huangqi Zhechong decoction can inhibit hepatic fibrosis resulted from chronic liver injure, retard the development of cirrhosis, and notably ameliorate the liver function. It may be a safe and effective therapeutic drug for patients with fibrosis.
文摘Dot-Enzyme-linked Immunosorbent Assay (Dot-ELISA) was employed for the detection of serum ant/sporm antibodies (AsAb). We found that in 140 cases of infertile
文摘The antifertility effect of epididymal injection of Sodium Morrhuate ( SM ) via the vas deferens as a new contraceptive method for males is definitely positive. However, the mechanism of its action has not been well understood. Further research in this field
文摘Objective: To detect the serum granulocyte colony-stimulating factor (G-CSF) levels between the patients with frequently repeated infection (repeaters) and others (non-repeaters) in different phase of infection. Methods: An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF levels in 50 cases (32 non-repeaters and 18 repeaters) with acute phase of infection. Serum G-CSF levels were detected in recovery phase in 10 cases. Results: Serum G-CSF levels were significantly higher (1429.97±506.43ng/L) in 32 non-repeaters with acute infection. There was a positive correlation between white blood cell count (WBC) and serum G-CSF level (r =0. 396, P <0.05). There was also a positive correlation between absolute neutrophil count (ANC) and serum G-CSF level (r=0.346,P<0,05). Serum G-CSF levels were higher (98.62 -+56.40ng/L) in 18 repeaters with acute infection, It was showed that serum G-CSF levels were significantly higher in non-repeaters than in repeaters with acute phase of infection ( P <0. 001 ). In the meanwhile, the body temperature was significantly higher in non-repeaters than in repeaters with acute infection (37.95±0.14℃ rs 36.91±0.13℃ ,P <0.001), There were no significant differences in age, WBC, ANC, type of bacterial, liver function and renal function (P > 0.05). Serum G-CSF levels in recovery phase of the two groups were below the sensitivity of the assay ( < 60 ng/L), Conlusion: It is suggested that application of recombinant G-CSF may be useful for the patients with repeated infection,
基金Supported by Grants from Health Department of Hubei Province (JX2B09)NSFC (30771911)National 973 Key Program (2009CB522502), and Tongji Hospital
文摘AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expressed as a GST-fusion protein, and interaction of HCV E2 protein with a tupaia CD81 LEL was evaluated by enzyme-linked immunosorbent assay (EIA). RESULTS: Although tupaia and human CD81 LEL differed in 6 amino acid changes, tupaia CD81 LEL was strongly recognized by anti-CD81 antibodies against human CD81 LEL conformation-dependent epitopes. Investigating LEL CD81-E2 interactions by EIA, we demonstrated that binding of tupaia CD81 LEL GST fusion protein to recombinant HCV E2 protein was markedly reduced compared to binding of human CD81 LEL GST fusion protein to recombinant HCV E2 protein. CONCLUSION: These data suggest that the structural differences in-between the tupaia and human CD81 may alter the interaction of the large extracellular loop with HCV envelope glycoprotein E2. These findings may be important for the understanding of the mechanisms of binding and entry of HCV to PTHs.
文摘AIM： To investigate possibility and clinical application of fecal calprotectin in determining disease activity of ulcerative colitis （UC）. METHODS： The enzyme-linked immunosorbent assay （ELISA） was used to measure the concentrations of calprotectin in feces obtained from 66 patients with UC and 20 controls. C-reactive protein （CRP）, erythrocyte sedimentation rate （ESR）, acid glycoprotein （AGP） were also measured and were compared with calprotectin in determining disease activity of UC. The disease activity of UC was also determined by the Sutherland criteria. RESULTS： The fecal calprotectin concentration in the patients with active UC was significantly higher than that in the inactive UC and in the controls （402.16 ± 48.0 μg/g vs 35.93 ± 3.39 μg/g, 11.5 ± 3.42 μg/g, P 〈 0.01）. The fecal calprotectin concentration in the inactive UC group was significantly higher than that in the control group （P 〈 0.05）. A significant difference was also found in the patients with active UC of mild, moderate and severe degrees. The area under the curve of the receiver operating characteristics （AUCR^c） was 0.975, 0.740, 0.692 and 0.737 for fecal calprotectin, CRP, ESR and AGP, respectively. There was a strong correlation between the fecal calprotectin concentration and the endoscopic gradings for UC （r = 0.866, P 〈 0.001）. CONCLUSION： Calprotectin in the patient＇s feces can reflect the disease activity of UC and can be used as a rational fecal marker for intestinal inflammation in clinical practice. This kind of marker is relatively precise, simple and noninvasive when compared with other commonlyused markers such as CRP, ESR and AGP.
文摘AIM:To assess Helicobacter pylori(Hpylon)seroprevalence in a cohort of Greek patients with lung cancer. METHODS:Seventy-two lung cancer patients(55 males and 17 females,aged 58.2±11.7 years)and 68,age and gender-matched,control subjects were enrolled.All subjects underwent an enzyme-linked immunosorbent assay IgG serologic test for Hpylori diagnosis. RESULTS:A correlation between age and HpyloriIgG level was detected for both lung cancer patients(r=0.42, P=0.004)and controls(r=0.44,P=0.004).Seropositivity for Hpyloridid not differ significantly between patients with lung cancer and controls(61.1% vs 55.9%,P>0.05). Concerning the mean serum concentration of IgG antibodies against Hpylori,no significant difference between the two groups was detected(32.6±19.1 vs 27.4±18.3 U/mL, P>0.05). CONCLUSION:No significant association between Hpylori infection and lung cancer was found.
