Miniaturization of electronic equipment has forced researchers to devise more effective methods for dissipating the generated heat in these devices.In this study,two methods,including porous media inserting and adding...Miniaturization of electronic equipment has forced researchers to devise more effective methods for dissipating the generated heat in these devices.In this study,two methods,including porous media inserting and adding nanoparticles to the base fluid,are used to improve heat transfer in an annulus heated on both walls.To study porous media insert,porous ribs are used on the outer and inner walls independently.The results show that when porous ribs are placed on the outer wall,although the heat transfer enhances,the pressure drop increment is so considerable that performance number (the ratio of heat transfer enhancement pressure increment,PN) is less than unity for all porous rib heights and porous media permeabilities that are studied.On the other hand,the PN of cases where porous ribs were placed on the inner wall depends on the Darcy number (Da).For example,for ribs with Da=0.1 and Da=0.0001,the maximum performance number,PN=4,occurs at the porous ribs height to hydraulic diameter ratios H/Dh=1 and H/Dh=0.25.Under these conditions,heat transfer is enhanced by two orders of magnitude.It is found that adding 5% nanoparticles to the base fluid in the two aforementioned cases improves the Nusselt number and PN by 10%–40%.展开更多
In this paper, we report on the comprehensive alcohol-/ion-responsive properties of a smart copolymer poly(N- isopropylacry]amide-co-benzo-18-crown-6-acrylamide) (P(NIPAM-co-BCAm)). The orthogonal design method ...In this paper, we report on the comprehensive alcohol-/ion-responsive properties of a smart copolymer poly(N- isopropylacry]amide-co-benzo-18-crown-6-acrylamide) (P(NIPAM-co-BCAm)). The orthogonal design method is adopted for experimental design. The experimental results show that alcohol can trigger the shrinking and Ba2t can induce the swelling of the P(NIPAM-co-BCAm) copolymer. According to the phase transition tempera- ture (LCST) change results of the copolymer, the influence of variables on the LCST changes weakens in the following order: alcohol concentration 〉 alcohol species 〉 metal ion species 〉 BCAm concentration 〉 ion concentration. The larger the alcohol concentration and the larger the molecular size of alcohols, the lower the LCST value; on the contrary, the more the BCAm content in the copolymer or the larger the BCAm/ion complex stability constant (IgK) or the larger the ion concentration is, the higher the LCST value. For a P(NIPAM-co-BCAm ) copolymer with a fixed BCAm content, a binary function of ion concentration and IgK of BCAm/ion is developed to precisely predict the LCST values of the copolymer in different metal ion solutions. The results provide valuable information for fabricating artificial biomimetic G-protein-gated inwardly rectifying potassium (GIRK) channels that are activated by alcohol and inhibited by Ba2+.展开更多
We recently reported that zacopride is a selective inward rectifier potassium current (IK1 ) channel agonist, suppressing ventricular arrhythmias without affecting atrial arrhythmias. The present study aimed to invest...We recently reported that zacopride is a selective inward rectifier potassium current (IK1 ) channel agonist, suppressing ventricular arrhythmias without affecting atrial arrhythmias. The present study aimed to investigate the unique pharmacological properties of zacopride. The whole-cell patch-clamp technique was used to study IK1 currents in rat atrial myocytes and Kir2.x currents in human embryonic kidney (HEK)-293 cells transfected with inward rectifier potassium channel (Kir)2.1, Kir2.2, Kir2.3, or mutated Kir2.1 (at phosphorylation site S425L). Western immunoblots were performed to estimate the relative protein expression levels of Kir2.x in rat atria and ventricles. Results showed that zacopride did not affect the IK1 and transmembrane potential of atrial myocytes. In HEK293 cells, zacopride increased Kir2.1 homomeric channels by 40.7%±9.7% at 50 mV, but did not affect Kir2.2 and Kir2.3 homomeric channels, and Kir2.1-Kir2.2, Kir2.1-Kir2.3 and Kir2.2-Kir2.3 heteromeric channels. Western immunoblots showed that similar levels of Kir2.3 protein were expressed in rat atria and ventricles, but atrial Kir2.1 protein level was only 25% of that measured in the ventricle. In addition, 5-hydroxytryptamine (5-HT) 3 receptor was undetectable, whereas 5-HT 4 receptor was weakly expressed in HEK293 cells. The Kir2.1-activating effect of zacopride in these cells was abolished by inhibition of protein kinase A (PKA), but not PKC or PKG. Furthermore, zacopride did not activate the mutant Kir2.1 channel in HEK293 cells but selectively activated the Kir2.1 homomeric channel via a PKA-dependent pathway, independent to that of the 5-HT receptor.展开更多
文摘Miniaturization of electronic equipment has forced researchers to devise more effective methods for dissipating the generated heat in these devices.In this study,two methods,including porous media inserting and adding nanoparticles to the base fluid,are used to improve heat transfer in an annulus heated on both walls.To study porous media insert,porous ribs are used on the outer and inner walls independently.The results show that when porous ribs are placed on the outer wall,although the heat transfer enhances,the pressure drop increment is so considerable that performance number (the ratio of heat transfer enhancement pressure increment,PN) is less than unity for all porous rib heights and porous media permeabilities that are studied.On the other hand,the PN of cases where porous ribs were placed on the inner wall depends on the Darcy number (Da).For example,for ribs with Da=0.1 and Da=0.0001,the maximum performance number,PN=4,occurs at the porous ribs height to hydraulic diameter ratios H/Dh=1 and H/Dh=0.25.Under these conditions,heat transfer is enhanced by two orders of magnitude.It is found that adding 5% nanoparticles to the base fluid in the two aforementioned cases improves the Nusselt number and PN by 10%–40%.
基金Supported by the National Natural Science Foundation of China(21136006)the Foundation for the Author of National Excellent Doctoral Dissertation of PR China(201163)the National High Technology Research and Development Program(2012AA021403)
文摘In this paper, we report on the comprehensive alcohol-/ion-responsive properties of a smart copolymer poly(N- isopropylacry]amide-co-benzo-18-crown-6-acrylamide) (P(NIPAM-co-BCAm)). The orthogonal design method is adopted for experimental design. The experimental results show that alcohol can trigger the shrinking and Ba2t can induce the swelling of the P(NIPAM-co-BCAm) copolymer. According to the phase transition tempera- ture (LCST) change results of the copolymer, the influence of variables on the LCST changes weakens in the following order: alcohol concentration 〉 alcohol species 〉 metal ion species 〉 BCAm concentration 〉 ion concentration. The larger the alcohol concentration and the larger the molecular size of alcohols, the lower the LCST value; on the contrary, the more the BCAm content in the copolymer or the larger the BCAm/ion complex stability constant (IgK) or the larger the ion concentration is, the higher the LCST value. For a P(NIPAM-co-BCAm ) copolymer with a fixed BCAm content, a binary function of ion concentration and IgK of BCAm/ion is developed to precisely predict the LCST values of the copolymer in different metal ion solutions. The results provide valuable information for fabricating artificial biomimetic G-protein-gated inwardly rectifying potassium (GIRK) channels that are activated by alcohol and inhibited by Ba2+.
基金supported by the National Natural Science Foundation of China (81170145 to Zhao ZhiQing, 31200864 to Liu QingHua, 31171088 to Cao JiMin)a Science and Technology Program fund from the Health Department of Shanxi Province (2011055 to Zhang Li)
文摘We recently reported that zacopride is a selective inward rectifier potassium current (IK1 ) channel agonist, suppressing ventricular arrhythmias without affecting atrial arrhythmias. The present study aimed to investigate the unique pharmacological properties of zacopride. The whole-cell patch-clamp technique was used to study IK1 currents in rat atrial myocytes and Kir2.x currents in human embryonic kidney (HEK)-293 cells transfected with inward rectifier potassium channel (Kir)2.1, Kir2.2, Kir2.3, or mutated Kir2.1 (at phosphorylation site S425L). Western immunoblots were performed to estimate the relative protein expression levels of Kir2.x in rat atria and ventricles. Results showed that zacopride did not affect the IK1 and transmembrane potential of atrial myocytes. In HEK293 cells, zacopride increased Kir2.1 homomeric channels by 40.7%±9.7% at 50 mV, but did not affect Kir2.2 and Kir2.3 homomeric channels, and Kir2.1-Kir2.2, Kir2.1-Kir2.3 and Kir2.2-Kir2.3 heteromeric channels. Western immunoblots showed that similar levels of Kir2.3 protein were expressed in rat atria and ventricles, but atrial Kir2.1 protein level was only 25% of that measured in the ventricle. In addition, 5-hydroxytryptamine (5-HT) 3 receptor was undetectable, whereas 5-HT 4 receptor was weakly expressed in HEK293 cells. The Kir2.1-activating effect of zacopride in these cells was abolished by inhibition of protein kinase A (PKA), but not PKC or PKG. Furthermore, zacopride did not activate the mutant Kir2.1 channel in HEK293 cells but selectively activated the Kir2.1 homomeric channel via a PKA-dependent pathway, independent to that of the 5-HT receptor.