目的应用悬浮培养转基因西洋参冠瘿组织生物合成熊果苷。方法向预培养20 d的西洋参冠瘿组织培养物中加入60 m g/mL对苯二酚溶液,共培养60 h后终止转化;通过TLC和HPLC方法检测产物,使用柱色谱法分离纯化产物,利用光谱数据对其结构进行鉴...目的应用悬浮培养转基因西洋参冠瘿组织生物合成熊果苷。方法向预培养20 d的西洋参冠瘿组织培养物中加入60 m g/mL对苯二酚溶液,共培养60 h后终止转化;通过TLC和HPLC方法检测产物,使用柱色谱法分离纯化产物,利用光谱数据对其结构进行鉴定。HPLC定量分析熊果苷产生的动力学变化曲线。结果产物同时在冠瘿组织培养物和培养基存在,并经过提取、分离得到其纯品,经理化性质和谱学方法将其鉴定为4-羟基苯-β-D-吡喃葡萄糖苷(熊果苷)。摩尔转化率在共培养60 h后达到最高(88.4%),同时,培养物和培养基中产物的量(63.69 m g/瓶,1.86 m g/瓶)以及产物的分泌百分率都达到最高(2.92%)。结论在国内外首次将转基因冠瘿组织用于生物转化系统并成功地得到具有多种药理活性的熊果苷。展开更多
AIM To determine the dynamics of growth and total tanshinones accumulation in crown gall cultures of Salvia miltiorrhiza in MS and 67 V liquid media. METHODS Fresh, dry weight and total tanshinones yields in the cultu...AIM To determine the dynamics of growth and total tanshinones accumulation in crown gall cultures of Salvia miltiorrhiza in MS and 67 V liquid media. METHODS Fresh, dry weight and total tanshinones yields in the cultures and in the medium were determined every 5 days in crown gall suspension cultures. RESULTS In MS medium, the logarithmic growth phase of crown gall cultures in S.miltiorrhiza was from the 5th to 30th days, and the stationary growth phase was from the 30th to 35th days. From the 25th to 30th days, physiological activity of crown gall cultures was higher and their growth was better. However, in 67 V medium, the logarithmic growth phase of crown gall cultures was from the 10th to 25th days, and the stationary growth phase was from the 25th to 35th days. Total tanshinones were largely accumulated in the cultures and in the medium after 25 days. The total tanshinones yield (60 mg·L -1 ) was reached at the 35th day. CONCLUSION Knowing the regularity of the growth and total tanshinones accumulation in crown gall cultures of S.miltiorrhiza will be helpful to take proper regulative measures in order to obtain the maximum total tanshinones yield.展开更多
目的:在西洋参冠瘿组织固体培养的基础上,进一步考察其悬浮培养条件及人参总皂苷含量,为利用西洋参冠瘿组织大规模生产药用成分提供科学根据。方法:考察固体培养和悬浮培养对西洋参冠瘿组织生长和人参总皂苷累积的影响,并检测悬浮培养...目的:在西洋参冠瘿组织固体培养的基础上,进一步考察其悬浮培养条件及人参总皂苷含量,为利用西洋参冠瘿组织大规模生产药用成分提供科学根据。方法:考察固体培养和悬浮培养对西洋参冠瘿组织生长和人参总皂苷累积的影响,并检测悬浮培养液中碳源的消耗。结果:冠瘿组织在悬浮培养中生长速度快,生长量和总皂苷产量分别达到0.776 g DW/flask和19.4 mg/flask,是固体培养的2.11和1.64倍;培养基中蔗糖利用率达到91.8%,远远高于西洋参愈伤组织悬浮培养中的蔗糖利用率。结论:西洋参冠瘿组织悬浮培养既可大大提高生物工程产品的生物量,又能获得较高含量的人参总皂苷,可为大规模生产药用活性物质提供参考。展开更多
2008年，对酸枣冠瘿病在新疆阿克苏红枣栽植区的发生危害进行了调查，结果表明：酸枣1年生实生苗肿瘤主要发生于根颈部位，多数苗木每株1个肿瘤，肿瘤平均直径1．3cm，发病率为3％～5％，病情指数为1．75～2．10。分离获得的160个可疑...2008年，对酸枣冠瘿病在新疆阿克苏红枣栽植区的发生危害进行了调查，结果表明：酸枣1年生实生苗肿瘤主要发生于根颈部位，多数苗木每株1个肿瘤，肿瘤平均直径1．3cm，发病率为3％～5％，病情指数为1．75～2．10。分离获得的160个可疑菌株，绝大多数存在于皮层组织，木质部很少。根据菌落特征将其归为4种，2种为优势细菌，其分离比率占总菌株的95％。通过症状观察、病原菌分离及菌落培养特征、革兰氏染色等，并通过西红柿接种快速诱导根系产生肿瘤，确定引起当地酸枣冠瘿病的病原为Agrobacterium tumefaciens（Smith and Townsend）Conn．。展开更多
文摘目的应用悬浮培养转基因西洋参冠瘿组织生物合成熊果苷。方法向预培养20 d的西洋参冠瘿组织培养物中加入60 m g/mL对苯二酚溶液,共培养60 h后终止转化;通过TLC和HPLC方法检测产物,使用柱色谱法分离纯化产物,利用光谱数据对其结构进行鉴定。HPLC定量分析熊果苷产生的动力学变化曲线。结果产物同时在冠瘿组织培养物和培养基存在,并经过提取、分离得到其纯品,经理化性质和谱学方法将其鉴定为4-羟基苯-β-D-吡喃葡萄糖苷(熊果苷)。摩尔转化率在共培养60 h后达到最高(88.4%),同时,培养物和培养基中产物的量(63.69 m g/瓶,1.86 m g/瓶)以及产物的分泌百分率都达到最高(2.92%)。结论在国内外首次将转基因冠瘿组织用于生物转化系统并成功地得到具有多种药理活性的熊果苷。
文摘AIM To determine the dynamics of growth and total tanshinones accumulation in crown gall cultures of Salvia miltiorrhiza in MS and 67 V liquid media. METHODS Fresh, dry weight and total tanshinones yields in the cultures and in the medium were determined every 5 days in crown gall suspension cultures. RESULTS In MS medium, the logarithmic growth phase of crown gall cultures in S.miltiorrhiza was from the 5th to 30th days, and the stationary growth phase was from the 30th to 35th days. From the 25th to 30th days, physiological activity of crown gall cultures was higher and their growth was better. However, in 67 V medium, the logarithmic growth phase of crown gall cultures was from the 10th to 25th days, and the stationary growth phase was from the 25th to 35th days. Total tanshinones were largely accumulated in the cultures and in the medium after 25 days. The total tanshinones yield (60 mg·L -1 ) was reached at the 35th day. CONCLUSION Knowing the regularity of the growth and total tanshinones accumulation in crown gall cultures of S.miltiorrhiza will be helpful to take proper regulative measures in order to obtain the maximum total tanshinones yield.
文摘目的:在西洋参冠瘿组织固体培养的基础上,进一步考察其悬浮培养条件及人参总皂苷含量,为利用西洋参冠瘿组织大规模生产药用成分提供科学根据。方法:考察固体培养和悬浮培养对西洋参冠瘿组织生长和人参总皂苷累积的影响,并检测悬浮培养液中碳源的消耗。结果:冠瘿组织在悬浮培养中生长速度快,生长量和总皂苷产量分别达到0.776 g DW/flask和19.4 mg/flask,是固体培养的2.11和1.64倍;培养基中蔗糖利用率达到91.8%,远远高于西洋参愈伤组织悬浮培养中的蔗糖利用率。结论:西洋参冠瘿组织悬浮培养既可大大提高生物工程产品的生物量,又能获得较高含量的人参总皂苷,可为大规模生产药用活性物质提供参考。
文摘2008年，对酸枣冠瘿病在新疆阿克苏红枣栽植区的发生危害进行了调查，结果表明：酸枣1年生实生苗肿瘤主要发生于根颈部位，多数苗木每株1个肿瘤，肿瘤平均直径1．3cm，发病率为3％～5％，病情指数为1．75～2．10。分离获得的160个可疑菌株，绝大多数存在于皮层组织，木质部很少。根据菌落特征将其归为4种，2种为优势细菌，其分离比率占总菌株的95％。通过症状观察、病原菌分离及菌落培养特征、革兰氏染色等，并通过西红柿接种快速诱导根系产生肿瘤，确定引起当地酸枣冠瘿病的病原为Agrobacterium tumefaciens（Smith and Townsend）Conn．。
