目的 对碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）增强重组人骨形态发生蛋白-2（recombinant human bone morphogenetic protein-2,rhBMP-2）诱导成骨能力的剂量依赖性进行研究。方法280只BALB/c小鼠随机分为8...目的 对碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）增强重组人骨形态发生蛋白-2（recombinant human bone morphogenetic protein-2,rhBMP-2）诱导成骨能力的剂量依赖性进行研究。方法280只BALB/c小鼠随机分为8组,每组35只,实验组rhBMP-2的剂量均为0.5mg,bFGF的剂量分别为0、2、10、40、80、300、500活性单位（IU）;设立单纯牛松质骨载体组为对照组。按实验设计,分别将rhBMP-2混悬液及bFGF-PVP溶液与牛松质骨载体充分混匀,负压下真空抽吸,冻干。将复合植骨材料植入小鼠右侧股部肌袋内,各组分别于术后第3、5、7、9、14、21d六个时间点取材,进行组织学、X线分析以及钙含量测定,观察各组的诱导成骨情况。结果（1）bFGF剂量为10～80IU时,间充质细胞增殖、分化,软骨细胞及新生骨形成均早于其它各组,成骨量多于其它各组,组织钙含量明显高于其它各组（P<0.05）,提示此剂量bFGF使rhBMP-2的诱导成骨能力明显增强。（2）bFGF剂量为0.2、300 IU时,各组在间充质细胞增殖、分化,软骨细胞及新生骨形成以及组织钙含量方面差异均无显著性意义（P>0.05）,提示此剂量bhBMP-2的诱导成骨能力无明显影响。（3）bFGF剂量为500IU时,术后第21d,仅1例标本可见散在的极少量新骨形成。展开更多
The elicitation response in allergic contact dermatitis for the hair dye substance p-phenylenediamine (PPD) is dose dependent,but threshold concentrations have not previously been investigated. 15 PPD-sensitive patien...The elicitation response in allergic contact dermatitis for the hair dye substance p-phenylenediamine (PPD) is dose dependent,but threshold concentrations have not previously been investigated. 15 PPD-sensitive patients participated in a serial dilution 48-hr patch test with PPD using 8 concentrations of PPD ranging from 1 to 10 000 on the upper back. Petrolatum was applied as control. Three concentrations (50, 100 and 500 p.p.m. PPD) were also applied to the retroauricular area and on the lateral aspects of the upper arms. 14 of the 15 participants reacted to one or more of the test samples. The threshold value for 10%of the tested persons (ED10) based on+or stronger reactions for PPD on the back was 38 p.p.m. (CI:4.3-100). There were no statistically significant differences in the sensitivity of the three anatomical regions. The upper back is a suitable region for patch testing patients with hair dye dermatitis.展开更多
文摘目的 对碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）增强重组人骨形态发生蛋白-2（recombinant human bone morphogenetic protein-2,rhBMP-2）诱导成骨能力的剂量依赖性进行研究。方法280只BALB/c小鼠随机分为8组,每组35只,实验组rhBMP-2的剂量均为0.5mg,bFGF的剂量分别为0、2、10、40、80、300、500活性单位（IU）;设立单纯牛松质骨载体组为对照组。按实验设计,分别将rhBMP-2混悬液及bFGF-PVP溶液与牛松质骨载体充分混匀,负压下真空抽吸,冻干。将复合植骨材料植入小鼠右侧股部肌袋内,各组分别于术后第3、5、7、9、14、21d六个时间点取材,进行组织学、X线分析以及钙含量测定,观察各组的诱导成骨情况。结果（1）bFGF剂量为10～80IU时,间充质细胞增殖、分化,软骨细胞及新生骨形成均早于其它各组,成骨量多于其它各组,组织钙含量明显高于其它各组（P<0.05）,提示此剂量bFGF使rhBMP-2的诱导成骨能力明显增强。（2）bFGF剂量为0.2、300 IU时,各组在间充质细胞增殖、分化,软骨细胞及新生骨形成以及组织钙含量方面差异均无显著性意义（P>0.05）,提示此剂量bhBMP-2的诱导成骨能力无明显影响。（3）bFGF剂量为500IU时,术后第21d,仅1例标本可见散在的极少量新骨形成。
文摘The elicitation response in allergic contact dermatitis for the hair dye substance p-phenylenediamine (PPD) is dose dependent,but threshold concentrations have not previously been investigated. 15 PPD-sensitive patients participated in a serial dilution 48-hr patch test with PPD using 8 concentrations of PPD ranging from 1 to 10 000 on the upper back. Petrolatum was applied as control. Three concentrations (50, 100 and 500 p.p.m. PPD) were also applied to the retroauricular area and on the lateral aspects of the upper arms. 14 of the 15 participants reacted to one or more of the test samples. The threshold value for 10%of the tested persons (ED10) based on+or stronger reactions for PPD on the back was 38 p.p.m. (CI:4.3-100). There were no statistically significant differences in the sensitivity of the three anatomical regions. The upper back is a suitable region for patch testing patients with hair dye dermatitis.
