Flaviviral NS2B is a required cofactor for NS3 serine protease activity and plays an important role in promoting functional NS2B-NS3 protease configuration and maintaining critical interactions with protease catalysis...Flaviviral NS2B is a required cofactor for NS3 serine protease activity and plays an important role in promoting functional NS2B-NS3 protease configuration and maintaining critical interactions with protease catalysis substrates. The residues D80DDG in West Nile virus (WNV) NS2B are important for protease activity. To investigate the effects of D80DDG in NS2B on protease activity and viral replication, the negatively charged region D80DD and the conserved residue G83 of NS2B were mutated (D80DD/E80EE, D80DD/K80KK, D80DD/A80AA, G83F, G83S, G83D, G83K, and G83A), and NS3 D75A was designated as the negative control. The effects of the mutations on NS2B-NS3 activity, viral translation, and viral RNA replication were analyzed using kinetic analysis of site-directed enzymes and a transient replicon assay. All substitutions resulted in significantly decreased enzyme activity and blocked RNA replication. The negative charge of D80DD is not important for maintaining NS2B function, but side chain changes in G83 have dramatic effects on protease activity and RNA replication. These results demonstrate that NS2B is important for viral replication and that D80DD and G83 substitutions prevent replication; they will be useful for understanding the relationship between NS2B and NS3.展开更多
In this paper, a new method called dynamic finite layer--element method (DFLEM) is used to analyse the soil--structure interaction of new type offshore platforms. Some valuable results have been obtained. The results ...In this paper, a new method called dynamic finite layer--element method (DFLEM) is used to analyse the soil--structure interaction of new type offshore platforms. Some valuable results have been obtained. The results show that the DFLEM is a new effective method to analyse dynamic soil--structure interaction and can be applied widely in practice.展开更多
The reverse construction and analysis of the networks of molecular interactions are essential for understanding their functions within cells. In this paper, a logic network model is constructed to investigate the comp...The reverse construction and analysis of the networks of molecular interactions are essential for understanding their functions within cells. In this paper, a logic network model is constructed to investigate the complicated regulation mechanism of shoot genes of Arabidopsis Thaliana in response to stimuli. The dynamics of the complicated logic network is analyzed, discussed, and simulated. The simulation results show that the logic network of the active genes of shoot eventually evolves into eleven attractors under the stimuli, including five 1-periodic and six 2-periodic attractors. Our work provides valuable reference and guidance for biologists to understand and explain Arabidopsis＇ response to external stimuli by experiments.展开更多
基金Supported by Important National Science& Technology Specific Projects (2012ZX10004403,2012ZX10004219)
文摘Flaviviral NS2B is a required cofactor for NS3 serine protease activity and plays an important role in promoting functional NS2B-NS3 protease configuration and maintaining critical interactions with protease catalysis substrates. The residues D80DDG in West Nile virus (WNV) NS2B are important for protease activity. To investigate the effects of D80DDG in NS2B on protease activity and viral replication, the negatively charged region D80DD and the conserved residue G83 of NS2B were mutated (D80DD/E80EE, D80DD/K80KK, D80DD/A80AA, G83F, G83S, G83D, G83K, and G83A), and NS3 D75A was designated as the negative control. The effects of the mutations on NS2B-NS3 activity, viral translation, and viral RNA replication were analyzed using kinetic analysis of site-directed enzymes and a transient replicon assay. All substitutions resulted in significantly decreased enzyme activity and blocked RNA replication. The negative charge of D80DD is not important for maintaining NS2B function, but side chain changes in G83 have dramatic effects on protease activity and RNA replication. These results demonstrate that NS2B is important for viral replication and that D80DD and G83 substitutions prevent replication; they will be useful for understanding the relationship between NS2B and NS3.
文摘In this paper, a new method called dynamic finite layer--element method (DFLEM) is used to analyse the soil--structure interaction of new type offshore platforms. Some valuable results have been obtained. The results show that the DFLEM is a new effective method to analyse dynamic soil--structure interaction and can be applied widely in practice.
基金This research is supported by the National Natural Science Foundation of China under Grant Nos. 60874036 and 60503002.
文摘The reverse construction and analysis of the networks of molecular interactions are essential for understanding their functions within cells. In this paper, a logic network model is constructed to investigate the complicated regulation mechanism of shoot genes of Arabidopsis Thaliana in response to stimuli. The dynamics of the complicated logic network is analyzed, discussed, and simulated. The simulation results show that the logic network of the active genes of shoot eventually evolves into eleven attractors under the stimuli, including five 1-periodic and six 2-periodic attractors. Our work provides valuable reference and guidance for biologists to understand and explain Arabidopsis＇ response to external stimuli by experiments.
