目的:基于网络药理学方法探究半夏厚朴汤治疗慢性咽炎的作用机制。方法:通过TCMSP (中药系统药理学技术平台)数据库获取“半夏”、“厚朴”、“紫苏”、“茯苓”和“生姜”的主要化学成分,根据ADME筛选出其活性成分及作用靶点,并上传Cyt...目的:基于网络药理学方法探究半夏厚朴汤治疗慢性咽炎的作用机制。方法:通过TCMSP (中药系统药理学技术平台)数据库获取“半夏”、“厚朴”、“紫苏”、“茯苓”和“生姜”的主要化学成分,根据ADME筛选出其活性成分及作用靶点,并上传Cytoscape 3.7.2软件构建“药物–成分–靶点网络”。同时通过Gencards、OMIM和DRUGBANK数据库获取慢性咽炎的主要靶点,用在线数据分析作图网站制作Venn图,并将所得交集靶点导入Cytoscape 3.8.2构建“药物–有效成分–靶点–疾病”网络。然后运用STRING数据库绘制蛋白互作(PPI)网络,结果导入Cytoscape软件3.8.2进行网络拓扑分析。最后利用Matescape数据库对交集靶点进行GO功能、KEGG通路富集分析,采用Cytoscape3.8.2构建“靶点–通路”网络。结果:筛选出半夏厚朴汤有效化学成分44个,对应靶点157个,慢性咽炎靶点1288个,半夏厚朴汤慢性咽炎交集靶点85个。分析得半夏厚朴汤治疗慢性咽炎的重要活性成分主要是木犀草素、β-谷甾醇、豆甾醇、黄芩、宽松、卡维定、桉油醇、黄芩苷、塞雷维甾醇,TP53、AKT1、JUN、CASP3、TNF、EGFR、IL6等。GO功能富集分析得到2479项结果,其中BP 2249项,MF 129项、CC 101项。KEGG通路分析得到177条信号通路。结论:半夏厚朴汤治疗慢性咽炎的多成分、多靶点、多通路的作用机制,为治疗慢性咽炎提供了网络药理学依据。Objective: Based on the method of network pharmacology to explore the mechanism of Banxia Houpu decoction in the treatment of chronic pharyngitis. Methods: The main chemical components of Pinellia pinellia, Magnolia officinalis, Perilla, Poria cocos and ginger were obtained from TCMSP database, and the active components and target sites were selected based on ADME, and the drug-composition-target network was constructed by uploaded Cytoscape 3.7.2 software. Meanwhile, major targets of chronic pharyngitis were obtained from Gencards, OMIM and DRUGBANK databases, and Venn diagram was prepared using online data analysis and mapping website. The obtained intersection targets were imported into Cytoscape 3.8.2 to build a “drug-activity-target-disease” network. Then the STRING database is used to draw the PPI network. The results were imported into Cytoscape software 3.8.2 for network topology analysis. Finally, GO function and KEGG pathway enrichment analysis were conducted on the intersection target using Matescape database, and Cytoscape3.8.2 was used to construct the “target-pathway” network. Results: 44 effective chemical components of Pinxia Houpu decoction were screened, 157 corresponding targets, 1288 chronic pharyngitis targets, 85 chronic pharyngitis intersection targets of Pinxia Houpu decoction. Analysis showed that the main active ingredients of Pinellia Houpu decoction for chronic pharyngitis were luteolin, β-sitosterol, stigmasterol, baicalin, carvetin, cineolitol, baicalin, serevisterol, TP53, AKT1, JUN, CASP3, TNF, EGFR, IL6, etc. GO functional enrichment analysis obtained 2479 results, including BP 2249, MF 129 and CC 101. KEGG pathway analysis showed 177 signaling pathways. Conclusion: The multi-component, multi-target and multi-pathway mechanism of Pinellia Magnolia decoction in the treatment of chronic pharyngitis provides the network pharmacological basis for Pinellia Magnolia decoction in the treatment of chronic pharyngitis.展开更多
文摘目的探讨咳喘定穴位贴敷联合小柴胡汤合半夏厚朴汤治疗痰浊阻肺证慢性阻塞性肺疾病(Chronic obstructive pulmonary disease,COPD)患者的临床疗效。方法选取2020年9月—2022年10月期间海南医学院第二附属医院收治的痰浊阻肺证COPD患者144例,按随机数字表法分为西药组、中药组、中药联合贴敷组,每组各48例。西药组采取常规西医治疗,中药组在西药基础上采取小柴胡汤合半夏厚朴汤治疗,中药联合贴敷组在常规西医基础上采取咳喘定穴位贴敷及小柴胡汤合半夏厚朴汤。治疗4周后,观察比较3组患者临床疗效、不良反应发生率、疾病复发率及治疗前后中医证候积分、肺功能[第1秒用力呼气容积(Forced expiratory volume for 1 second,FEV_(1))、用力肺活量(Forced vital capacity,FVC)、FEV_(1)/FVC]、康复效果[慢性阻塞性肺疾病评估测试量表(Chronic obstructive Pulmonary Disease Assessment Test Scale,CAT)、呼吸困难程度评分(modified Medical Research Council,mMRC)、6 min步行距离试验(6 min walking distance test,6MWT)]。结果治疗后中药联合贴敷组总有效率93.75%(45/48)高于西药组77.08%(37/48)、中药组79.17%(38/48),差异有统计学意义(P<0.05)。治疗后3组患者中医证候痰多、喘息、咳嗽、倦怠乏力、腹胀纳呆、下肢水肿积分均较治疗前降低,差异有统计学意义(P<0.05),且中药联合贴敷组中医证候痰多、喘息、咳嗽、倦怠乏力、腹胀纳呆、下肢水肿积分均明显低于西药组、中药组,差异有统计学意义(P<0.05)。治疗后3组患者肺功能FEV_(1)、FVC、FEV_(1)/FVC指标均较治疗前升高,差异有统计学意义(P<0.05);且中药联合贴敷组肺功能FEV_(1)、FVC、FEV_(1)/FVC指标明显高于西药组、中药组,差异有统计学意义(P<0.05)。治疗后3组患者康复效果CAT、mMRC评分较治疗前降低,6MWT较治疗前延长,差异有统计学意义(P<0.05);且中药联合贴敷组康复效果CAT、mMRC评分均明显低于西药组、中药组,6MWT明显优于西药组、中药组,差异有统计学意义(P<0.05)。治疗期间,中药联合贴敷组不良反应发生率8.33%(4/48)与西药组6.25%(3/48)、中药组4.17%(2/48)比较,差异无统计学意义(P>0.05)。治疗6个月后中药联合贴敷组疾病复发率4.17%(2/48)低于西药组18.75%(9/48)、中药组16.67(8/48),差异有统计学意义(P<0.05)。结论联合采取咳喘定穴位贴敷及小柴胡汤合半夏厚朴汤治疗痰浊阻肺证COPD效果显著,可有效缓解患者临床症状,改善肺功能及机体运动耐力,利于提升疾病整体干预效果,降低疾病复发率,且具有安全性。
文摘目的:基于网络药理学方法探究半夏厚朴汤治疗慢性咽炎的作用机制。方法:通过TCMSP (中药系统药理学技术平台)数据库获取“半夏”、“厚朴”、“紫苏”、“茯苓”和“生姜”的主要化学成分,根据ADME筛选出其活性成分及作用靶点,并上传Cytoscape 3.7.2软件构建“药物–成分–靶点网络”。同时通过Gencards、OMIM和DRUGBANK数据库获取慢性咽炎的主要靶点,用在线数据分析作图网站制作Venn图,并将所得交集靶点导入Cytoscape 3.8.2构建“药物–有效成分–靶点–疾病”网络。然后运用STRING数据库绘制蛋白互作(PPI)网络,结果导入Cytoscape软件3.8.2进行网络拓扑分析。最后利用Matescape数据库对交集靶点进行GO功能、KEGG通路富集分析,采用Cytoscape3.8.2构建“靶点–通路”网络。结果:筛选出半夏厚朴汤有效化学成分44个,对应靶点157个,慢性咽炎靶点1288个,半夏厚朴汤慢性咽炎交集靶点85个。分析得半夏厚朴汤治疗慢性咽炎的重要活性成分主要是木犀草素、β-谷甾醇、豆甾醇、黄芩、宽松、卡维定、桉油醇、黄芩苷、塞雷维甾醇,TP53、AKT1、JUN、CASP3、TNF、EGFR、IL6等。GO功能富集分析得到2479项结果,其中BP 2249项,MF 129项、CC 101项。KEGG通路分析得到177条信号通路。结论:半夏厚朴汤治疗慢性咽炎的多成分、多靶点、多通路的作用机制,为治疗慢性咽炎提供了网络药理学依据。Objective: Based on the method of network pharmacology to explore the mechanism of Banxia Houpu decoction in the treatment of chronic pharyngitis. Methods: The main chemical components of Pinellia pinellia, Magnolia officinalis, Perilla, Poria cocos and ginger were obtained from TCMSP database, and the active components and target sites were selected based on ADME, and the drug-composition-target network was constructed by uploaded Cytoscape 3.7.2 software. Meanwhile, major targets of chronic pharyngitis were obtained from Gencards, OMIM and DRUGBANK databases, and Venn diagram was prepared using online data analysis and mapping website. The obtained intersection targets were imported into Cytoscape 3.8.2 to build a “drug-activity-target-disease” network. Then the STRING database is used to draw the PPI network. The results were imported into Cytoscape software 3.8.2 for network topology analysis. Finally, GO function and KEGG pathway enrichment analysis were conducted on the intersection target using Matescape database, and Cytoscape3.8.2 was used to construct the “target-pathway” network. Results: 44 effective chemical components of Pinxia Houpu decoction were screened, 157 corresponding targets, 1288 chronic pharyngitis targets, 85 chronic pharyngitis intersection targets of Pinxia Houpu decoction. Analysis showed that the main active ingredients of Pinellia Houpu decoction for chronic pharyngitis were luteolin, β-sitosterol, stigmasterol, baicalin, carvetin, cineolitol, baicalin, serevisterol, TP53, AKT1, JUN, CASP3, TNF, EGFR, IL6, etc. GO functional enrichment analysis obtained 2479 results, including BP 2249, MF 129 and CC 101. KEGG pathway analysis showed 177 signaling pathways. Conclusion: The multi-component, multi-target and multi-pathway mechanism of Pinellia Magnolia decoction in the treatment of chronic pharyngitis provides the network pharmacological basis for Pinellia Magnolia decoction in the treatment of chronic pharyngitis.
