The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2...The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement,and the better ones are: acetosyringone concentration,30 mg·L-1;infection time,15 minutes;and culturing conditions of strains,temperature 28 ℃,28 hours shaking culture under dark environment with shaking.Pre-experiment on tissue culture system of peony was conducted,and got better medium composition as: WP+6-BA 0.5 mg·L-1+ IBA 0.1 mg·L-1+GA30.5 mg·L-1for base buds,and MS + 6-BA 2.0 mg·L-1+IBA 0.1 mg·L-1+GA3 0.5 mg·L-1for stem segments.展开更多
文摘The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement,and the better ones are: acetosyringone concentration,30 mg·L-1;infection time,15 minutes;and culturing conditions of strains,temperature 28 ℃,28 hours shaking culture under dark environment with shaking.Pre-experiment on tissue culture system of peony was conducted,and got better medium composition as: WP+6-BA 0.5 mg·L-1+ IBA 0.1 mg·L-1+GA30.5 mg·L-1for base buds,and MS + 6-BA 2.0 mg·L-1+IBA 0.1 mg·L-1+GA3 0.5 mg·L-1for stem segments.
