991440 大菱鲆水呼吸道肠道病毒(TRV)在细胞培养中的复制和形态发生=Repli-cation and morphogenesis of the turbotaquaceovirus(TRV)in cell culture [刊,英]/Rivas C,Noya M//Aquac..—1998,160(1/2).—47～62利用 CHSE-214细胞研究...991440 大菱鲆水呼吸道肠道病毒(TRV)在细胞培养中的复制和形态发生=Repli-cation and morphogenesis of the turbotaquaceovirus(TRV)in cell culture [刊,英]/Rivas C,Noya M//Aquac..—1998,160(1/2).—47～62利用 CHSE-214细胞研究大菱鲆水呼吸道肠道病毒(TRV)复制周期的超显微结构。虽然这种病毒的复制和形态发生与典型的呼吸道肠道病毒基本相似,但它有一些重要的差异。病毒粒子直接穿过质膜进入宿主细胞内(甚至在水温低于4℃以下)。在受感染细胞的细胞质中发现两种大小的病毒粒子:(1)直径30nm 的粒子,可能相当于核心,位于细胞质病毒质里面;(2)直径45nm 的粒子,位于内质网的泡囊中,可能相当于单壳病毒粒子。展开更多
Beijing has been severely affected by SARS, and SARS-associated coronavirus has been confirmed as its cause. However, clinical and experimental evidence implicates the possibility of co-infection. In this report, reov...Beijing has been severely affected by SARS, and SARS-associated coronavirus has been confirmed as its cause. However, clinical and experimental evidence implicates the possibility of co-infection. In this report, reovirus was isolated from throat swabs of SARS patients, including the first case in Beijing and her mother. Identification with the electron microscopy revealed the characteristic features of reovirus. 24 of 38 samples from other SARS cases were found to have serologic responses to the reovirus. Primers designed for reovirus have amplified several fragments of DNA, one of which was sequenced (S2 gene fragment), which indicates it as a unique reovirus (orthoreovirus). Preliminary animal experiment showed that inoculation of the reovirus in mice caused death with atypical pneumonia. Nevertheless, the association of reovirus with SARS outbreak requires to be further investigated.展开更多
文摘991440 大菱鲆水呼吸道肠道病毒(TRV)在细胞培养中的复制和形态发生=Repli-cation and morphogenesis of the turbotaquaceovirus(TRV)in cell culture [刊,英]/Rivas C,Noya M//Aquac..—1998,160(1/2).—47～62利用 CHSE-214细胞研究大菱鲆水呼吸道肠道病毒(TRV)复制周期的超显微结构。虽然这种病毒的复制和形态发生与典型的呼吸道肠道病毒基本相似,但它有一些重要的差异。病毒粒子直接穿过质膜进入宿主细胞内(甚至在水温低于4℃以下)。在受感染细胞的细胞质中发现两种大小的病毒粒子:(1)直径30nm 的粒子,可能相当于核心,位于细胞质病毒质里面;(2)直径45nm 的粒子,位于内质网的泡囊中,可能相当于单壳病毒粒子。
基金supported by the National High Technology Research and Development Program of China(Grant No.2001AA233041).
文摘Beijing has been severely affected by SARS, and SARS-associated coronavirus has been confirmed as its cause. However, clinical and experimental evidence implicates the possibility of co-infection. In this report, reovirus was isolated from throat swabs of SARS patients, including the first case in Beijing and her mother. Identification with the electron microscopy revealed the characteristic features of reovirus. 24 of 38 samples from other SARS cases were found to have serologic responses to the reovirus. Primers designed for reovirus have amplified several fragments of DNA, one of which was sequenced (S2 gene fragment), which indicates it as a unique reovirus (orthoreovirus). Preliminary animal experiment showed that inoculation of the reovirus in mice caused death with atypical pneumonia. Nevertheless, the association of reovirus with SARS outbreak requires to be further investigated.
