Comparative experiments of the induction of buds and roots from the explants of cotyledon, hypocotyl and young leaves of \%Hypericum perforatum \%L. under the different kinds and concentrations of hormones were carrie...Comparative experiments of the induction of buds and roots from the explants of cotyledon, hypocotyl and young leaves of \%Hypericum perforatum \%L. under the different kinds and concentrations of hormones were carried out \%in vitro. \%The results indicated that bud differentiation of explants on MS medium with BA and NAA could be observed, and the ratio of BA to NAA influenced the organogenesis of explants to a great extent. When the concentration of BA was 3 mg/L, the optimal concentration of NAA for bud differentiation of cotyledon, hypocotyl and young leaves were 0.2 mg/L, 0.1 mg/L, 1.0 mg/L respectively. Rooting was observed when NAA 1.0 mg/L or the combination of NAA 0.5 mg/L with IBA 0.5 mg/L supplemented to the MS medium, and eventually grew into a complete plantlet.展开更多
在改良 B_5培养基上,枣叶片愈伤组织的形成主要受细胞分裂素 BA 的影响,浓度在100mg/1时,培养20天诱导率达100%,生长素 IBA 只起调节愈伤组织生长状态的作用;愈伤组织的器官分化受 IBA 和 BA 浓度的影响很大,其分化类型取决于 IBA/BA ...在改良 B_5培养基上,枣叶片愈伤组织的形成主要受细胞分裂素 BA 的影响,浓度在100mg/1时,培养20天诱导率达100%,生长素 IBA 只起调节愈伤组织生长状态的作用;愈伤组织的器官分化受 IBA 和 BA 浓度的影响很大,其分化类型取决于 IBA/BA 的浓度比值。展开更多
文摘Comparative experiments of the induction of buds and roots from the explants of cotyledon, hypocotyl and young leaves of \%Hypericum perforatum \%L. under the different kinds and concentrations of hormones were carried out \%in vitro. \%The results indicated that bud differentiation of explants on MS medium with BA and NAA could be observed, and the ratio of BA to NAA influenced the organogenesis of explants to a great extent. When the concentration of BA was 3 mg/L, the optimal concentration of NAA for bud differentiation of cotyledon, hypocotyl and young leaves were 0.2 mg/L, 0.1 mg/L, 1.0 mg/L respectively. Rooting was observed when NAA 1.0 mg/L or the combination of NAA 0.5 mg/L with IBA 0.5 mg/L supplemented to the MS medium, and eventually grew into a complete plantlet.
