Caveolin-2,a protein about 20 kD,is a major component of the inner surface of caveolae,small invaginations of the plasma membrane.Similar with caveolin-1 and caveolin-3,it serves as a protein marker of caveolae.Caveol...Caveolin-2,a protein about 20 kD,is a major component of the inner surface of caveolae,small invaginations of the plasma membrane.Similar with caveolin-1 and caveolin-3,it serves as a protein marker of caveolae.Caveolin-1 and-2 are located next to each other at 7q31.1 on human chromosome,the proteins encoded are co-localized and form a stable hetero-oligomeric complex,distributing similarly in tissue and cultured cells.Caveolin-3 is located on different chromosomes but confirmed to interact with caveolin-2.Caveolin-2 is similar to caveolin-1 in many respects but differs from the latter in functional domains,especially in G-protein binding domain and caveolin scaffolding domain.The mRNAs of both caveolin-1 and caveolin-2 are most abundantly expressed in white adipose tissue and are induced during differentiation of 3T3-L1 cells to adipocytes.Caveolin-2-deficinet mice demonstrate clear pulmonary defects,with little or no change in caveolin-1 expression and caveolae formation,suggesting that caveolin-2 plays a selective role in lung functions.Caveolin-2 is also involved in lipid metabolism and human cancers.展开更多
Microalgae Tetraselmis subcordiformis and Nannochloropsis oculata were cultured at 15,20,25,30,and 35℃ and their properties as potential biofuel resources were examined.The results indicate that T.subcordiformis and ...Microalgae Tetraselmis subcordiformis and Nannochloropsis oculata were cultured at 15,20,25,30,and 35℃ and their properties as potential biofuel resources were examined.The results indicate that T.subcordiformis and N.oculata grew best at 20℃ and 25℃ and yielded the highest total lipids at 20℃and 30℃,respectively.With increased temperature,neutral lipid and polyunsaturated fatty acids(FAs)decreased while saturated FAs increased,accompanied by increased monounsaturated FAs(MUFAs) in T.subcordiformis and decreased MUFAs in N.oculata;meanwhile,the predicted cetane number of FA methyl esters increased from 45.3 to 47.6 in T.subcordiformis and from 52.3 to 60.3 in N.oculata.Therefore,optimizing culture temperatures is important for improving microalgal biodiesel production.展开更多
In situ evaluation of cell cultivation on degrading poly(ε-caprolactone) (PCL) films was studied.New culture surroundings were constructed for cell growth by using PCL films as substrates and adding Pseudomonas cepac...In situ evaluation of cell cultivation on degrading poly(ε-caprolactone) (PCL) films was studied.New culture surroundings were constructed for cell growth by using PCL films as substrates and adding Pseudomonas cepacia lipase to accelerate biodegradation of PCL films.MTT experiments for 10 h indicated the low cytotoxicity of lipase solution with concentration up to 0.2 mg/mL for MG-63 cells growth on PCL films.With the optimized lipase concentration and degradation time,we studied cell growth behavior on dynamically changed PCL films by adding lipase to the culture surroundings.MTT,fluorescence microscopy and scanning electron microscopy (SEM) were used to evaluate cell viability,proliferation and morphologies.It was found that cell viability and proliferation were not affected by the added lipase solution negatively.In contrast,cells cultured on degrading PCL films showed good growth behavior with clear fusiform shape and pseudopods.Importantly,the enzymatic degradation of PCL films with cells attachment showed distinctive morphology compared to the degradation in lipase solution without cells.The simultaneous cell growth and PCL film degradation were well discussed in this work,which may better understand the interaction between cell growth and polymer degradation.展开更多
基金Supported by National Basic Research Program of China (973 Program) (2004CB518602,2006CB503909)National High Technology Research and Development Program of China (863 Program) (2009AA02Z192)
文摘Caveolin-2,a protein about 20 kD,is a major component of the inner surface of caveolae,small invaginations of the plasma membrane.Similar with caveolin-1 and caveolin-3,it serves as a protein marker of caveolae.Caveolin-1 and-2 are located next to each other at 7q31.1 on human chromosome,the proteins encoded are co-localized and form a stable hetero-oligomeric complex,distributing similarly in tissue and cultured cells.Caveolin-3 is located on different chromosomes but confirmed to interact with caveolin-2.Caveolin-2 is similar to caveolin-1 in many respects but differs from the latter in functional domains,especially in G-protein binding domain and caveolin scaffolding domain.The mRNAs of both caveolin-1 and caveolin-2 are most abundantly expressed in white adipose tissue and are induced during differentiation of 3T3-L1 cells to adipocytes.Caveolin-2-deficinet mice demonstrate clear pulmonary defects,with little or no change in caveolin-1 expression and caveolae formation,suggesting that caveolin-2 plays a selective role in lung functions.Caveolin-2 is also involved in lipid metabolism and human cancers.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2009AA064401)the Special Fundation for Marine Renewable Energy from the State Oceanic Administration of China(No.SHME2011SW02)the Shanghai Universities First-class Disciplines Project of Fisheries(A)
文摘Microalgae Tetraselmis subcordiformis and Nannochloropsis oculata were cultured at 15,20,25,30,and 35℃ and their properties as potential biofuel resources were examined.The results indicate that T.subcordiformis and N.oculata grew best at 20℃ and 25℃ and yielded the highest total lipids at 20℃and 30℃,respectively.With increased temperature,neutral lipid and polyunsaturated fatty acids(FAs)decreased while saturated FAs increased,accompanied by increased monounsaturated FAs(MUFAs) in T.subcordiformis and decreased MUFAs in N.oculata;meanwhile,the predicted cetane number of FA methyl esters increased from 45.3 to 47.6 in T.subcordiformis and from 52.3 to 60.3 in N.oculata.Therefore,optimizing culture temperatures is important for improving microalgal biodiesel production.
基金supported by the National Natural Science Foundation of China (50573085 & 50521302) and Chinese Academy of Sciences
文摘In situ evaluation of cell cultivation on degrading poly(ε-caprolactone) (PCL) films was studied.New culture surroundings were constructed for cell growth by using PCL films as substrates and adding Pseudomonas cepacia lipase to accelerate biodegradation of PCL films.MTT experiments for 10 h indicated the low cytotoxicity of lipase solution with concentration up to 0.2 mg/mL for MG-63 cells growth on PCL films.With the optimized lipase concentration and degradation time,we studied cell growth behavior on dynamically changed PCL films by adding lipase to the culture surroundings.MTT,fluorescence microscopy and scanning electron microscopy (SEM) were used to evaluate cell viability,proliferation and morphologies.It was found that cell viability and proliferation were not affected by the added lipase solution negatively.In contrast,cells cultured on degrading PCL films showed good growth behavior with clear fusiform shape and pseudopods.Importantly,the enzymatic degradation of PCL films with cells attachment showed distinctive morphology compared to the degradation in lipase solution without cells.The simultaneous cell growth and PCL film degradation were well discussed in this work,which may better understand the interaction between cell growth and polymer degradation.
