Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA ...Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.展开更多
An optimal design methodology for the configuration of two rail slider was proposed to get better dynamic performance. The taper length, taper height and the rail width of the reading/writing head are considered as ...An optimal design methodology for the configuration of two rail slider was proposed to get better dynamic performance. The taper length, taper height and the rail width of the reading/writing head are considered as design variables. The complex geometry method is utilized as the search scheme in the optimization process. Optimization results show that the new slider has better dynamic characteristics and is more stable than the original designed slider. The optimization process also demonstrates that the optimum model and optimum method is effective.展开更多
Bioremediation plays an increasingly important role in the remediation of chromium-contaminated soil because it is an environmentally friendly technology. To investigate the Cr(Ⅵ)reduction process by indigenous micro...Bioremediation plays an increasingly important role in the remediation of chromium-contaminated soil because it is an environmentally friendly technology. To investigate the Cr(Ⅵ)reduction process by indigenous microorganisms in soil, a batch of incubation experiments were carried out in a bioreactor under aerobic conditions. The results showed that in the presence of indigenous microorganisms, the Cr(Ⅵ) concentration in the chromium-contaminated soil decreased from 1521.9 to 199.2 mg/kg within 66 h with culture medium addition, while a slight decrease in the Cr(Ⅵ) concentration was found in the sterilized soil,implying that the indigenous microorganisms contributed to the Cr(Ⅵ) reduction. In the microbial remediation process, Cr(Ⅵ)microbial reduction occurred after the reduction of NO3-, Mn4+ and Fe3+ and,before SO42- reduction. The reduction process of Cr(Ⅵ) can be divided into two phases, characterized by the exponential equation model of microbial reduction and the linear equation model of the combined effect of the major ions. It can be concluded that indigenous Cr(Ⅵ)-reducing bacteria have a potential application for in-situ remediation of Cr(Ⅵ)-contaminated soil.展开更多
Based on the structure of glass (or liquid) polymers consisting of α-domain, β-co-domain, and entanglement constituent chain networks, and the nonexponentially viscoelastic behavior, a “heterophase fluctuation” ...Based on the structure of glass (or liquid) polymers consisting of α-domain, β-co-domain, and entanglement constituent chain networks, and the nonexponentially viscoelastic behavior, a “heterophase fluctuation” model was proposed. It was found that the dynamics of cooperative rearrangement on the “fluidized domain” has a great shear rate, domain size, and temperature dependences. When the shear rate, domain size, and temperature dependences were taken account into the cooperatively localized rearrangement on the fluidized domain by the degradation of primary α-domain and the reformation of secondary β-co-domain constituent chains. A new dynamic theory of cooperatively localized rearrangement on the fluidized domain constituent chains with different size and different network chain length during physical and mechanical aging was established. The total viscoelastic free en-ergy of deformation resulting from the change in conformations of α-domain, β-co-domain, crytallite, crosslinked, and trapped entanglement constituent chains during aging processes was calculated by the combining method of kinetics and statistical mechanics. The constitu- tive equations and reduced stress relaxation modulus and creep compliances for three types of polymers were also derived. Finally, two reduced universal equations on creep compliance and stress relaxation modulus with a non-linear and two nonexponential parameters α and β were theoretically derived from the dynamic theory and a statistically extended mode coupling theory for double aging effects of polymers was developed. Results show that the two reduced universal equations have the same form as Kohlraush-Williams-Watts (K-W-W) stretched exponential function. The nonlinearity and the nonexponentiality are, respectively, originated from the memory effects of nonthermal and thermal history. The correlation of nonlinearity, α and β to the aging time, aging temperature, and the mesomorphic structure of fluidized domains was also established.展开更多
Coxsackievirus A16(CVA16),together with enterovirus type 71(EV71),is responsible for most cases of hand,foot and mouth disease(HFMD) worldwide.Recent findings suggest that the recombination between CVA16 and EV71,and ...Coxsackievirus A16(CVA16),together with enterovirus type 71(EV71),is responsible for most cases of hand,foot and mouth disease(HFMD) worldwide.Recent findings suggest that the recombination between CVA16 and EV71,and the co-circulation of these two viruses may have contributed to the increase of HFMD cases in China over the past few years.It is therefore important to further understand the virology,epidemiology,virus-host interactions and host pathogenesis of CVA16.In this study,we describe the viral kinetics of CVA16 in human rhabdomyosarcoma(RD) cells by analyzing the cytopathic effect(CPE),viral RNA replication,viral protein expression,viral RNA package and viral particle secretion in RD cells.We show that CVA16 appears to first attach,uncoat and enter into the host cell after adsorption for 1 h.Later on,CVA16 undergoes rapid replication from 3 to 6 h at MOI 1 and until 9 h at MOI 0.1.At MOI 0.1,CVA16 initiates a secondary infection as the virions were secreted before 9 h p.i.CPE was observed after 12 h p.i.,and viral antigen was first detected at 6 h p.i.at MOI 1 and at 9 h p.i.at MOI 0.1.Thus,our study provides important information for further investigation of CVA16 in order to better understand and ultimately control infections with this virus.展开更多
Poly(p-phenylenediamine)/chitosan (PPPDA/Chi) composite was prepared by in situ chemical oxidative polymerization of p-phenylenediamine (PPPDA) into chitosan (Chi) using ammonium persulphate (APS) as an oxidant. PPPDA...Poly(p-phenylenediamine)/chitosan (PPPDA/Chi) composite was prepared by in situ chemical oxidative polymerization of p-phenylenediamine (PPPDA) into chitosan (Chi) using ammonium persulphate (APS) as an oxidant. PPPDA and PPPDA/Chi composite were characterized by FT-IR spectra and SEM before and after copper loading. In batch adsorption method, the maximum removal of copper was experienced when 1 g/L of PPPDA and PPPDA/Chi composite dosages were used at pH 5.0 for PPPDA and 6.0 for PPPDA/Chi composite for 360 min for both sorbents. PPPDA showed adsorption capacity q<sub>e</sub><sup>max</sup> of 650 mg/g whereas its composite achieved q<sub>e</sub><sup>max</sup> of 573 mg/g. The experimental data correlate well with the Freundlich isotherm equation and the pseudo-second order kinetic model. The Cu(II), loaded PPPDA and its composite can be efficiently reused for as many as four cycles. The Cu(II)-loaded sorbents showed high antibacterial efficiency against Gram-positive and Gram-negative bacteria than their unloaded forms.展开更多
The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determineits function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO wer...The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determineits function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO were analyzedby indirect immunofluorescence staining with an antibody against phospho-H3. We found that the phosphorylationbegins at early prophase, and spreads throughout the chromosomes at late prophase. At metaphase, most of the phospho-H3 aggregates at the end of the condensed entity of chromosomes at equatorial plate. During anaphase and telophase,the fluorescent signal of phospho-H3 is detached from chromosomes into cytoplasm. At early anaphase, phospho-H3shows ladder bands between two sets of separated chromosome, and forms “sandwich-like structure” when the chro-mosomes condensed. With the cleavage progressing, the “ladders” of the histone contract into a bigger bright dot. Thenthe histone aggregates and some of compacted microtubules in the midbody region are composed into a “bar-like”complex to separate daughter cells. The daughter cells seal their plasma membrane along with the ends of the “bar”,inside which locates microtubules and modified histones, to finish the cytokinesis and keep the “bar complex” out of thecells. The specific distribution and kinetics of phospho-H3 in cytoplasm suggest that the modified histones may takepart in the formation of midbody and play a crucial role in cytokinesis.展开更多
The Finite Element Method was combined with the results from considerable analysis, producing a new kinetics analysis method of EFEM for a mechanism in truss, geared system, and assembled system. The equivalent princi...The Finite Element Method was combined with the results from considerable analysis, producing a new kinetics analysis method of EFEM for a mechanism in truss, geared system, and assembled system. The equivalent principle and the motive differential equation of the system were derived by using an equivalent element, a virtual inertia matrix, and a systematic force matrix. The element’s mass matrix expression in the two dimensional and three dimensional mechanisms of the equivalent element was determined. The equivalent mass matrixes fashion of the Jacobin matrix, generalized coordinate matrix, and equivalent forces matrix were also determined. It was validated by two examples that the new method was normal, simple and direct, and had a higher efficiency than alternative methods; this is regardless of whether traditional methods are used with differential equations and calculated by using a computer.展开更多
A new kind of inertial piezoelectric actuator for a micro in-pipe robot is proposed and studied. The actuator is composed of a body, corresponding to a mass rod, and four elastic legs. Each leg is a composite piezoele...A new kind of inertial piezoelectric actuator for a micro in-pipe robot is proposed and studied. The actuator is composed of a body, corresponding to a mass rod, and four elastic legs. Each leg is a composite piezoelectric bimorph beam, made up of a middle metal element, an upper and lower piezoelectric elements. The mechanism is driven by an asymmetric waveform voltage, such as saw-toothed waveform, and utilizes the dynamic relationship between the maximum static friction force and the inertial force. To study the actuator, firstly, the constituent equation of a composite piezoelectric bimorph under both applied voltage and external force was inferred by thermodynamics. Secondly, the dvnamic model of the actuator was established analyzing the relationship between the locomotive states, viz. displacement and velocity, and design parameters, such as piezoelectric strain constant, elastic modulus,length, width and thickness of the piezoelectric element, actuator mass, and driving vohage. At last, the dynamic equation was solved and the theoretical calculation of the inherent frequency was more consistent with the experimental data, which proved the rationality of the model. All these lay a theoretical foundation of the micro actuator parameter optimization and more research on a micro robot.展开更多
Cold-rolled Ti/Al laminated composites were annealed at 525−625℃for 0−128 h,and the interfacial microstructure evolution was investigated.The results indicate that only the TiAl_(3) phase was formed at the Ti/Al inte...Cold-rolled Ti/Al laminated composites were annealed at 525−625℃for 0−128 h,and the interfacial microstructure evolution was investigated.The results indicate that only the TiAl_(3) phase was formed at the Ti/Al interface;most of TiAl_(3) grains were fine equiaxed with average sizes ranging from hundreds of nanometers to several microns and the TiAl_(3) grain size increased with increasing annealing time and/or temperature,but the effect of annealing temperature on the TiAl_(3) grain size was far greater than that of annealing time.The growth of the TiAl_(3) phase consisted of two stages.The initial stage was governed by chemical reaction with a reaction activation energy of 195.75 kJ/mol,and the reaction rate constant of the TiAl_(3) phase was larger as the Ti/Al interface was bonded with fresh surfaces.At the second stage,the growth was governed by diffusion,the diffusion activation energy was 33.69 kJ/mol,and the diffusion growth rate constant of the TiAl_(3) phase was mainly determined by the grain boundary diffusion owing to the smaller TiAl_(3) grain size.展开更多
基金Supported by Inner Mongolia Natural Science Foundation(200711020112)Innovation Fundation of Inner Mongolia University of Science and Technology (2009NC064)~~
文摘Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.
文摘An optimal design methodology for the configuration of two rail slider was proposed to get better dynamic performance. The taper length, taper height and the rail width of the reading/writing head are considered as design variables. The complex geometry method is utilized as the search scheme in the optimization process. Optimization results show that the new slider has better dynamic characteristics and is more stable than the original designed slider. The optimization process also demonstrates that the optimum model and optimum method is effective.
基金Project(2018SK2044)supported by the Innovation Program of Science&Technology of Hunan Province,ChinaProject(51304250)supported by the National Natural Science Foundation of China
文摘Bioremediation plays an increasingly important role in the remediation of chromium-contaminated soil because it is an environmentally friendly technology. To investigate the Cr(Ⅵ)reduction process by indigenous microorganisms in soil, a batch of incubation experiments were carried out in a bioreactor under aerobic conditions. The results showed that in the presence of indigenous microorganisms, the Cr(Ⅵ) concentration in the chromium-contaminated soil decreased from 1521.9 to 199.2 mg/kg within 66 h with culture medium addition, while a slight decrease in the Cr(Ⅵ) concentration was found in the sterilized soil,implying that the indigenous microorganisms contributed to the Cr(Ⅵ) reduction. In the microbial remediation process, Cr(Ⅵ)microbial reduction occurred after the reduction of NO3-, Mn4+ and Fe3+ and,before SO42- reduction. The reduction process of Cr(Ⅵ) can be divided into two phases, characterized by the exponential equation model of microbial reduction and the linear equation model of the combined effect of the major ions. It can be concluded that indigenous Cr(Ⅵ)-reducing bacteria have a potential application for in-situ remediation of Cr(Ⅵ)-contaminated soil.
基金ACKNOWLEDGMENT This work was supported by the National Natural Science Foundation of China (No.50973007).
文摘Based on the structure of glass (or liquid) polymers consisting of α-domain, β-co-domain, and entanglement constituent chain networks, and the nonexponentially viscoelastic behavior, a “heterophase fluctuation” model was proposed. It was found that the dynamics of cooperative rearrangement on the “fluidized domain” has a great shear rate, domain size, and temperature dependences. When the shear rate, domain size, and temperature dependences were taken account into the cooperatively localized rearrangement on the fluidized domain by the degradation of primary α-domain and the reformation of secondary β-co-domain constituent chains. A new dynamic theory of cooperatively localized rearrangement on the fluidized domain constituent chains with different size and different network chain length during physical and mechanical aging was established. The total viscoelastic free en-ergy of deformation resulting from the change in conformations of α-domain, β-co-domain, crytallite, crosslinked, and trapped entanglement constituent chains during aging processes was calculated by the combining method of kinetics and statistical mechanics. The constitu- tive equations and reduced stress relaxation modulus and creep compliances for three types of polymers were also derived. Finally, two reduced universal equations on creep compliance and stress relaxation modulus with a non-linear and two nonexponential parameters α and β were theoretically derived from the dynamic theory and a statistically extended mode coupling theory for double aging effects of polymers was developed. Results show that the two reduced universal equations have the same form as Kohlraush-Williams-Watts (K-W-W) stretched exponential function. The nonlinearity and the nonexponentiality are, respectively, originated from the memory effects of nonthermal and thermal history. The correlation of nonlinearity, α and β to the aging time, aging temperature, and the mesomorphic structure of fluidized domains was also established.
基金Partly supported by the National Natural Science Foundation of China (No. 20872048)
文摘Coxsackievirus A16(CVA16),together with enterovirus type 71(EV71),is responsible for most cases of hand,foot and mouth disease(HFMD) worldwide.Recent findings suggest that the recombination between CVA16 and EV71,and the co-circulation of these two viruses may have contributed to the increase of HFMD cases in China over the past few years.It is therefore important to further understand the virology,epidemiology,virus-host interactions and host pathogenesis of CVA16.In this study,we describe the viral kinetics of CVA16 in human rhabdomyosarcoma(RD) cells by analyzing the cytopathic effect(CPE),viral RNA replication,viral protein expression,viral RNA package and viral particle secretion in RD cells.We show that CVA16 appears to first attach,uncoat and enter into the host cell after adsorption for 1 h.Later on,CVA16 undergoes rapid replication from 3 to 6 h at MOI 1 and until 9 h at MOI 0.1.At MOI 0.1,CVA16 initiates a secondary infection as the virions were secreted before 9 h p.i.CPE was observed after 12 h p.i.,and viral antigen was first detected at 6 h p.i.at MOI 1 and at 9 h p.i.at MOI 0.1.Thus,our study provides important information for further investigation of CVA16 in order to better understand and ultimately control infections with this virus.
文摘Poly(p-phenylenediamine)/chitosan (PPPDA/Chi) composite was prepared by in situ chemical oxidative polymerization of p-phenylenediamine (PPPDA) into chitosan (Chi) using ammonium persulphate (APS) as an oxidant. PPPDA and PPPDA/Chi composite were characterized by FT-IR spectra and SEM before and after copper loading. In batch adsorption method, the maximum removal of copper was experienced when 1 g/L of PPPDA and PPPDA/Chi composite dosages were used at pH 5.0 for PPPDA and 6.0 for PPPDA/Chi composite for 360 min for both sorbents. PPPDA showed adsorption capacity q<sub>e</sub><sup>max</sup> of 650 mg/g whereas its composite achieved q<sub>e</sub><sup>max</sup> of 573 mg/g. The experimental data correlate well with the Freundlich isotherm equation and the pseudo-second order kinetic model. The Cu(II), loaded PPPDA and its composite can be efficiently reused for as many as four cycles. The Cu(II)-loaded sorbents showed high antibacterial efficiency against Gram-positive and Gram-negative bacteria than their unloaded forms.
文摘The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determineits function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO were analyzedby indirect immunofluorescence staining with an antibody against phospho-H3. We found that the phosphorylationbegins at early prophase, and spreads throughout the chromosomes at late prophase. At metaphase, most of the phospho-H3 aggregates at the end of the condensed entity of chromosomes at equatorial plate. During anaphase and telophase,the fluorescent signal of phospho-H3 is detached from chromosomes into cytoplasm. At early anaphase, phospho-H3shows ladder bands between two sets of separated chromosome, and forms “sandwich-like structure” when the chro-mosomes condensed. With the cleavage progressing, the “ladders” of the histone contract into a bigger bright dot. Thenthe histone aggregates and some of compacted microtubules in the midbody region are composed into a “bar-like”complex to separate daughter cells. The daughter cells seal their plasma membrane along with the ends of the “bar”,inside which locates microtubules and modified histones, to finish the cytokinesis and keep the “bar complex” out of thecells. The specific distribution and kinetics of phospho-H3 in cytoplasm suggest that the modified histones may takepart in the formation of midbody and play a crucial role in cytokinesis.
文摘The Finite Element Method was combined with the results from considerable analysis, producing a new kinetics analysis method of EFEM for a mechanism in truss, geared system, and assembled system. The equivalent principle and the motive differential equation of the system were derived by using an equivalent element, a virtual inertia matrix, and a systematic force matrix. The element’s mass matrix expression in the two dimensional and three dimensional mechanisms of the equivalent element was determined. The equivalent mass matrixes fashion of the Jacobin matrix, generalized coordinate matrix, and equivalent forces matrix were also determined. It was validated by two examples that the new method was normal, simple and direct, and had a higher efficiency than alternative methods; this is regardless of whether traditional methods are used with differential equations and calculated by using a computer.
基金Sponsored by the National Natural Science Foundation of China(Grant No.69774020)the National Doctoral Foundation of China(Grant No.98014106).
文摘A new kind of inertial piezoelectric actuator for a micro in-pipe robot is proposed and studied. The actuator is composed of a body, corresponding to a mass rod, and four elastic legs. Each leg is a composite piezoelectric bimorph beam, made up of a middle metal element, an upper and lower piezoelectric elements. The mechanism is driven by an asymmetric waveform voltage, such as saw-toothed waveform, and utilizes the dynamic relationship between the maximum static friction force and the inertial force. To study the actuator, firstly, the constituent equation of a composite piezoelectric bimorph under both applied voltage and external force was inferred by thermodynamics. Secondly, the dvnamic model of the actuator was established analyzing the relationship between the locomotive states, viz. displacement and velocity, and design parameters, such as piezoelectric strain constant, elastic modulus,length, width and thickness of the piezoelectric element, actuator mass, and driving vohage. At last, the dynamic equation was solved and the theoretical calculation of the inherent frequency was more consistent with the experimental data, which proved the rationality of the model. All these lay a theoretical foundation of the micro actuator parameter optimization and more research on a micro robot.
基金the financial supports from the S&T Program of Hebei Province,China(No.20373901D)the National Natural Science Foundation of China(Nos.51807047,51804095)+2 种基金the National Science Foundation of Hebei Province,China(No.E2019402433)the Youth Top Talents Science and Technology Research Project of Hebei Province University,China(No.BJ2019003)the Research and Development Project of Science and Technology of Handan City,China(No.19422111008-19).
文摘Cold-rolled Ti/Al laminated composites were annealed at 525−625℃for 0−128 h,and the interfacial microstructure evolution was investigated.The results indicate that only the TiAl_(3) phase was formed at the Ti/Al interface;most of TiAl_(3) grains were fine equiaxed with average sizes ranging from hundreds of nanometers to several microns and the TiAl_(3) grain size increased with increasing annealing time and/or temperature,but the effect of annealing temperature on the TiAl_(3) grain size was far greater than that of annealing time.The growth of the TiAl_(3) phase consisted of two stages.The initial stage was governed by chemical reaction with a reaction activation energy of 195.75 kJ/mol,and the reaction rate constant of the TiAl_(3) phase was larger as the Ti/Al interface was bonded with fresh surfaces.At the second stage,the growth was governed by diffusion,the diffusion activation energy was 33.69 kJ/mol,and the diffusion growth rate constant of the TiAl_(3) phase was mainly determined by the grain boundary diffusion owing to the smaller TiAl_(3) grain size.