遗传多样性的精准鉴定是资源可持续利用的基础。我们利用松属的3个种的叶绿体基因组序列中的物种特有的535个单核苷酸多态位点作为分子性状首次编制分子鉴定检索表,供试样品得到成功鉴定。物种特有的单核苷酸多态位点的数量和核苷酸构...遗传多样性的精准鉴定是资源可持续利用的基础。我们利用松属的3个种的叶绿体基因组序列中的物种特有的535个单核苷酸多态位点作为分子性状首次编制分子鉴定检索表,供试样品得到成功鉴定。物种特有的单核苷酸多态位点的数量和核苷酸构成存在种间差异。马尾松Pinus massoniana Lamb. (252)的特有单核苷酸多态位点的数量最多,随后依次是赤松P. densiflora Siebold & Zucc. (175)和黑松P. thunbergii Parl. (108)。马尾松的特有单核苷酸多态位点中,T的比例(29.37%)最高,随后依次是C (25.79%)、A (22.62%)和G (22.22%),G的比例最低,总体上差异较小。黑松的特有单核苷酸多态位点中,T (36.11%)的比例较C (12.96%)的比例高2.7倍,较G (20.37%)的比例高1.7倍;A (30.56%)的比例略低于T的比例,是G的1.5倍,是C的2.3倍。赤松的特有单核苷酸多态位点中,T (31.43%)和A (28.57%)的比例明显高于C (18.86%)或G (21.14%)的比例。结果显示,叶绿体基因组的单核苷酸多态位点信息,可用于松属植物资源遗传多样性的分子鉴定。调查了中国过去120多年来松属植物标本的采集和馆藏现状,讨论了存在的问题和对策。本研究对于松属植物的分类修订、种质资源的保护与利用具有重要价值。Accurate identification of genetic diversity is essential for sustainable utilization of plant resources. In this paper, 535 single nucleotide polymorphism (SNP) sites in the chloroplast genomes of 3 species from the genus Pinus L. were used as molecular traits to identify the plant genetic resource diversity of this plant genus and to compile a molecular classification key for the first time. There are differences in aspects of amount and base composition of SNPs among the species. The amount of SNPs in Pinus massoniana Lamb. (252) is the highest, being higher than that in P. densiflora Siebold & Zucc. (175) or P. thunbergii Parl. (108). In P. massoniana, the proportion of T (29.37%) is the highest, larger than that of C (25.79), and that of A (22.62%) or G (22.62%) is the lowest. In P. thunbergii, the proportion of T (36.11%) is 2.7 times that of C (12.96%), and 1.7 times that of G (20.37%). A (30.56%) is a little bit smaller than that of T, being 1.5 times that of G, and 2.3 times that of C. In P. densiflora, the proportion of T (31.43%) or A (28.57%) is significantly higher than that of C (18.86%) or G (21.14%). Our results indicated that single nucleotide polymorphism sites from the chloroplast genomes could be used for distinguishing different species successfully in the genus Pinus. The status of Pinus plant specimens collected in the past over 120 years in China is investigated, and problems and possible strategies are discussed. This study is valuable for taxonomic revision, conservation and utilization of Pinus plant germplasm resources.展开更多
5-HT1A基因编码5-羟色胺(血清素)的G蛋白偶联受体(属于5-羟色胺受体亚家族)。C-1019G多态位点是5-HT1A基因启动子区一个重要的功能性多态位点,人群中存在3种基因型即CC、CG、GG,它与个体的恋爱关系及抑郁症、焦虑症等精神疾病密切相关...5-HT1A基因编码5-羟色胺(血清素)的G蛋白偶联受体(属于5-羟色胺受体亚家族)。C-1019G多态位点是5-HT1A基因启动子区一个重要的功能性多态位点,人群中存在3种基因型即CC、CG、GG,它与个体的恋爱关系及抑郁症、焦虑症等精神疾病密切相关。本文对5-HT1A基因C-1019G多态位点的相关机制、检测方法、研究进展等进行综述。The 5-HT1A gene encodes a G protein-coupled receptor for serotonin, which belongs to the 5-hydroxytryptamine receptor subfamily. C-1019G polymorphism is an important functional poly-morphism in the promoter region of 5-HT1A gene. There are three genotypes (CC, CG and GG) in the population, which are closely related to individual’s romantic relationship and mental disorders such as depression and anxiety. In this paper, the mechanism, detection methods and research progress of C-1019G polymorphism of 5-HT1A gene were reviewed.展开更多
本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提...本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提供相应依据。采用测序技术对498只清远麻鸡VIPR-1基因5′调控区进行多态性检测及基因型分析,利用最小二乘均数对VIPR-1基因5′调控区的多态位点与就巢性状进行相关分析,利用PHASE软件对多态位点进行单倍型分析。结果,VIPR-1基因5′调控区存在12个多态性位点,G-359T、G-266T、A-134G、A-94G、C-72G对18～43周龄的就巢持续天数影响达到显著水平(P<0.05),A-94G位点对18～54周龄的就巢率的影响达到显著水平(P<0.05);构建的单倍型对18～54周龄的就巢持续天数的影响达到极显著水平(P<0.01)。最小二乘分析结果表明,CCTGGGAAGCAG/TTGGGGAAGCAC型个体的就巢持续天数比其它单倍型个体极显著的延长(P<0.01)。结果表明,VIPR-1基因5′调控区(-496～-1bp)可能存在影响鸡就巢行为的分子标记,CCTGGGAAGCAG/TTGGGGAAGCAC型个体对鸡就巢性具有较大的遗传效应,可作为分子标记应用于家鸡就巢行为的筛选。展开更多
高通量测序技术可低成本获得叶绿体全基因组序列,为落叶松属植物的精确鉴定提供了可能。我们利用叶绿体全基因组中的527个单核苷酸多态位点,作为分子性状,首次精准鉴定落叶松属8个种/变种,研制了分子分类检索表。单核苷酸多态位点存在...高通量测序技术可低成本获得叶绿体全基因组序列,为落叶松属植物的精确鉴定提供了可能。我们利用叶绿体全基因组中的527个单核苷酸多态位点,作为分子性状,首次精准鉴定落叶松属8个种/变种,研制了分子分类检索表。单核苷酸多态位点存在种间差异。喜马拉雅红杉(Larix himalaica W. C. Cheng & L. K. Fu)、新疆落叶松(L. sibirica Ledeb.)和欧洲落叶松(L. deciduas Mill.)的物种特有单核苷酸多态位点数量均较多,遗传分化较大。落叶松(原变种) (L. gmelinii (Rupr.) Kuzen. var. gmelinii)、黄花落叶松(L. gmelinii var. olgensis (A. Henry) Ostenf. & Syrach)、千岛落叶松(L. gmelinii var. japonica (Maxim. ex Regel) Pilg.)以及凯杨德落叶松(L. cajanderi Mayr)之间遗传差异较小。结果显示叶绿体基因组的单核苷酸多态位点信息,可用于落叶松属植物不同基因型的分子鉴定。本研究对于落叶松属植物种质资源的分类鉴定、保护和利用具有重要价值。展开更多
文摘遗传多样性的精准鉴定是资源可持续利用的基础。我们利用松属的3个种的叶绿体基因组序列中的物种特有的535个单核苷酸多态位点作为分子性状首次编制分子鉴定检索表,供试样品得到成功鉴定。物种特有的单核苷酸多态位点的数量和核苷酸构成存在种间差异。马尾松Pinus massoniana Lamb. (252)的特有单核苷酸多态位点的数量最多,随后依次是赤松P. densiflora Siebold & Zucc. (175)和黑松P. thunbergii Parl. (108)。马尾松的特有单核苷酸多态位点中,T的比例(29.37%)最高,随后依次是C (25.79%)、A (22.62%)和G (22.22%),G的比例最低,总体上差异较小。黑松的特有单核苷酸多态位点中,T (36.11%)的比例较C (12.96%)的比例高2.7倍,较G (20.37%)的比例高1.7倍;A (30.56%)的比例略低于T的比例,是G的1.5倍,是C的2.3倍。赤松的特有单核苷酸多态位点中,T (31.43%)和A (28.57%)的比例明显高于C (18.86%)或G (21.14%)的比例。结果显示,叶绿体基因组的单核苷酸多态位点信息,可用于松属植物资源遗传多样性的分子鉴定。调查了中国过去120多年来松属植物标本的采集和馆藏现状,讨论了存在的问题和对策。本研究对于松属植物的分类修订、种质资源的保护与利用具有重要价值。Accurate identification of genetic diversity is essential for sustainable utilization of plant resources. In this paper, 535 single nucleotide polymorphism (SNP) sites in the chloroplast genomes of 3 species from the genus Pinus L. were used as molecular traits to identify the plant genetic resource diversity of this plant genus and to compile a molecular classification key for the first time. There are differences in aspects of amount and base composition of SNPs among the species. The amount of SNPs in Pinus massoniana Lamb. (252) is the highest, being higher than that in P. densiflora Siebold & Zucc. (175) or P. thunbergii Parl. (108). In P. massoniana, the proportion of T (29.37%) is the highest, larger than that of C (25.79), and that of A (22.62%) or G (22.62%) is the lowest. In P. thunbergii, the proportion of T (36.11%) is 2.7 times that of C (12.96%), and 1.7 times that of G (20.37%). A (30.56%) is a little bit smaller than that of T, being 1.5 times that of G, and 2.3 times that of C. In P. densiflora, the proportion of T (31.43%) or A (28.57%) is significantly higher than that of C (18.86%) or G (21.14%). Our results indicated that single nucleotide polymorphism sites from the chloroplast genomes could be used for distinguishing different species successfully in the genus Pinus. The status of Pinus plant specimens collected in the past over 120 years in China is investigated, and problems and possible strategies are discussed. This study is valuable for taxonomic revision, conservation and utilization of Pinus plant germplasm resources.
文摘5-HT1A基因编码5-羟色胺(血清素)的G蛋白偶联受体(属于5-羟色胺受体亚家族)。C-1019G多态位点是5-HT1A基因启动子区一个重要的功能性多态位点,人群中存在3种基因型即CC、CG、GG,它与个体的恋爱关系及抑郁症、焦虑症等精神疾病密切相关。本文对5-HT1A基因C-1019G多态位点的相关机制、检测方法、研究进展等进行综述。The 5-HT1A gene encodes a G protein-coupled receptor for serotonin, which belongs to the 5-hydroxytryptamine receptor subfamily. C-1019G polymorphism is an important functional poly-morphism in the promoter region of 5-HT1A gene. There are three genotypes (CC, CG and GG) in the population, which are closely related to individual’s romantic relationship and mental disorders such as depression and anxiety. In this paper, the mechanism, detection methods and research progress of C-1019G polymorphism of 5-HT1A gene were reviewed.
文摘本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提供相应依据。采用测序技术对498只清远麻鸡VIPR-1基因5′调控区进行多态性检测及基因型分析,利用最小二乘均数对VIPR-1基因5′调控区的多态位点与就巢性状进行相关分析,利用PHASE软件对多态位点进行单倍型分析。结果,VIPR-1基因5′调控区存在12个多态性位点,G-359T、G-266T、A-134G、A-94G、C-72G对18～43周龄的就巢持续天数影响达到显著水平(P<0.05),A-94G位点对18～54周龄的就巢率的影响达到显著水平(P<0.05);构建的单倍型对18～54周龄的就巢持续天数的影响达到极显著水平(P<0.01)。最小二乘分析结果表明,CCTGGGAAGCAG/TTGGGGAAGCAC型个体的就巢持续天数比其它单倍型个体极显著的延长(P<0.01)。结果表明,VIPR-1基因5′调控区(-496～-1bp)可能存在影响鸡就巢行为的分子标记,CCTGGGAAGCAG/TTGGGGAAGCAC型个体对鸡就巢性具有较大的遗传效应,可作为分子标记应用于家鸡就巢行为的筛选。
文摘高通量测序技术可低成本获得叶绿体全基因组序列,为落叶松属植物的精确鉴定提供了可能。我们利用叶绿体全基因组中的527个单核苷酸多态位点,作为分子性状,首次精准鉴定落叶松属8个种/变种,研制了分子分类检索表。单核苷酸多态位点存在种间差异。喜马拉雅红杉(Larix himalaica W. C. Cheng & L. K. Fu)、新疆落叶松(L. sibirica Ledeb.)和欧洲落叶松(L. deciduas Mill.)的物种特有单核苷酸多态位点数量均较多,遗传分化较大。落叶松(原变种) (L. gmelinii (Rupr.) Kuzen. var. gmelinii)、黄花落叶松(L. gmelinii var. olgensis (A. Henry) Ostenf. & Syrach)、千岛落叶松(L. gmelinii var. japonica (Maxim. ex Regel) Pilg.)以及凯杨德落叶松(L. cajanderi Mayr)之间遗传差异较小。结果显示叶绿体基因组的单核苷酸多态位点信息,可用于落叶松属植物不同基因型的分子鉴定。本研究对于落叶松属植物种质资源的分类鉴定、保护和利用具有重要价值。
