A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm ...A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm in body size) without clinical signs. With primers 77012R and 77353F, a fragment of 356bp of IHHNV was amplified in RT PCR. The amplified product was sequenced and showed more than 98% homologue with the sequences of other IHHNV strains in Gene Bank.展开更多
文摘0 引言。乙型肝炎病毒(HBV)属嗜肝DNA病毒属，是一组以侵袭肝脏为主的病毒，主要感染哺乳动物和鸟类．HBV DNA的长度为3．2kb，具有4个开放读码框架(ORF)，分别编码HBV的表面抗原蛋白，核心／e抗原蛋白，X蛋白以及HBV DNA多聚酶(HBV DNA P)．HBV DNA P基因在ORF中最长，并且与C、S、X基因区有重叠，其编码的P蛋白含有N-末端蛋白(TP)、逆转录酶(RT)／DNA多聚酶、RNase H和隔离片(spacer，SP)等4个结构域，各结构域分别位于2307—2840nt、133—1128nt、1129—1621nt和2841—0—132nt．目前对多聚酶及其所编码的各个结构域的研究取得了一定的进展．
文摘A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm in body size) without clinical signs. With primers 77012R and 77353F, a fragment of 356bp of IHHNV was amplified in RT PCR. The amplified product was sequenced and showed more than 98% homologue with the sequences of other IHHNV strains in Gene Bank.
