过继性T细胞疗法(adoptive T cell therapy,ACT)是一种高度个性化的抗肿瘤治疗方法,临床研究正在迅速发展。研究表明,ACT疗法可以通过增加T细胞数量,增强对肿瘤组织的特异性和反应性,从而克服肿瘤免疫缺陷抑制的产生。ACT疗法目前有3种...过继性T细胞疗法(adoptive T cell therapy,ACT)是一种高度个性化的抗肿瘤治疗方法,临床研究正在迅速发展。研究表明,ACT疗法可以通过增加T细胞数量,增强对肿瘤组织的特异性和反应性,从而克服肿瘤免疫缺陷抑制的产生。ACT疗法目前有3种主要模式:肿瘤浸润淋巴细胞(TILs)、嵌合抗原受体T细胞(CAR-T)和T细胞受体工程化T细胞(TCR-T)治疗。本文对ACT疗法的分类及研究进展进行综述,包括TILs、CAR-T、TCR-T以及基于类器官共培养的过继性T淋巴细胞免疫治疗,探讨ACT作为头颈部鳞癌治疗方式的应用前景。展开更多
目的:探究肿瘤浸润免疫细胞(TIICs)浸润程度与头颈部鳞状细胞癌(HNSCC)患者预后的关系,并基于多种免疫细胞构建预后模型。方法:从TCGA数据库中下载515例HNSCC样本和44例正常对照样本的基因表达谱和生存信息,利用CIBERSORT算法计算每个...目的:探究肿瘤浸润免疫细胞(TIICs)浸润程度与头颈部鳞状细胞癌(HNSCC)患者预后的关系,并基于多种免疫细胞构建预后模型。方法:从TCGA数据库中下载515例HNSCC样本和44例正常对照样本的基因表达谱和生存信息,利用CIBERSORT算法计算每个样本中不同免疫细胞的占比,进行免疫细胞浸润分析和Kaplan-Meier生存分析。通过LASSO回归、单因素和多因素Cox回归筛选HNSCC样本中免疫细胞并构建免疫细胞风险评分预后模型,采用Kaplan-Meier法和ROC曲线评估该模型。结果:在22种TIICs中,M0、M1和M2巨噬细胞具有相对高的百分比,约占40%。CD8+T细胞与活化CD4+记忆T细胞呈显著正相关(r = 0.55),而与M0巨噬细胞(r = −0.53)呈显著负相关。生存分析表明高表达的调节性T细胞(P P P P Objective: This paper aims to investigate the relationship between the degree of tumor infiltrating immune cells (TIICs) and prognosis of head and neck squamous cell carcinoma (HNSCC) patients, and construct a prognostic model based on multiple immune cells. Methods: The gene expression profiles and survival information of 515 HNSCC samples and 44 normal control samples were downloaded from the TCGA database. The proportion of different immune cells in each sample was calculated using the CIBERSORT algorithm, and immune cell infiltration analysis and Kaplan-Meier survival analysis were performed. The immune cells in HNSCC samples were screened by LASSO regression, univariate and multivariate Cox regression, and an immune cell risk scoring prognostic model was constructed. The model was evaluated by Kaplan-Meier method and ROC curve. Results: Among the 22 TIICs, M0, M1, and M2 macrophages had relatively high percentages, accounting for about 40%. CD8+T cells were significantly positively correlated with activated CD4+ memory T cells (r = 0.55), while significantly negatively correlated with M0 macrophages (r = −0.53). Survival analysis showed that high expression of regulatory T cells (P P P P < 0.001). Conclusion: TIICs are closely related to the prognosis of HNSCC patients. Regulatory T cells may be considered as a target for treatment of HNSCC, and the prognostic model constructed in this study may provide reference for clinical prognosis.展开更多
文摘过继性T细胞疗法(adoptive T cell therapy,ACT)是一种高度个性化的抗肿瘤治疗方法,临床研究正在迅速发展。研究表明,ACT疗法可以通过增加T细胞数量,增强对肿瘤组织的特异性和反应性,从而克服肿瘤免疫缺陷抑制的产生。ACT疗法目前有3种主要模式:肿瘤浸润淋巴细胞(TILs)、嵌合抗原受体T细胞(CAR-T)和T细胞受体工程化T细胞(TCR-T)治疗。本文对ACT疗法的分类及研究进展进行综述,包括TILs、CAR-T、TCR-T以及基于类器官共培养的过继性T淋巴细胞免疫治疗,探讨ACT作为头颈部鳞癌治疗方式的应用前景。
文摘目的:探究肿瘤浸润免疫细胞(TIICs)浸润程度与头颈部鳞状细胞癌(HNSCC)患者预后的关系,并基于多种免疫细胞构建预后模型。方法:从TCGA数据库中下载515例HNSCC样本和44例正常对照样本的基因表达谱和生存信息,利用CIBERSORT算法计算每个样本中不同免疫细胞的占比,进行免疫细胞浸润分析和Kaplan-Meier生存分析。通过LASSO回归、单因素和多因素Cox回归筛选HNSCC样本中免疫细胞并构建免疫细胞风险评分预后模型,采用Kaplan-Meier法和ROC曲线评估该模型。结果:在22种TIICs中,M0、M1和M2巨噬细胞具有相对高的百分比,约占40%。CD8+T细胞与活化CD4+记忆T细胞呈显著正相关(r = 0.55),而与M0巨噬细胞(r = −0.53)呈显著负相关。生存分析表明高表达的调节性T细胞(P P P P Objective: This paper aims to investigate the relationship between the degree of tumor infiltrating immune cells (TIICs) and prognosis of head and neck squamous cell carcinoma (HNSCC) patients, and construct a prognostic model based on multiple immune cells. Methods: The gene expression profiles and survival information of 515 HNSCC samples and 44 normal control samples were downloaded from the TCGA database. The proportion of different immune cells in each sample was calculated using the CIBERSORT algorithm, and immune cell infiltration analysis and Kaplan-Meier survival analysis were performed. The immune cells in HNSCC samples were screened by LASSO regression, univariate and multivariate Cox regression, and an immune cell risk scoring prognostic model was constructed. The model was evaluated by Kaplan-Meier method and ROC curve. Results: Among the 22 TIICs, M0, M1, and M2 macrophages had relatively high percentages, accounting for about 40%. CD8+T cells were significantly positively correlated with activated CD4+ memory T cells (r = 0.55), while significantly negatively correlated with M0 macrophages (r = −0.53). Survival analysis showed that high expression of regulatory T cells (P P P P < 0.001). Conclusion: TIICs are closely related to the prognosis of HNSCC patients. Regulatory T cells may be considered as a target for treatment of HNSCC, and the prognostic model constructed in this study may provide reference for clinical prognosis.
