Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse tes...Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.展开更多
SIRT1(silent mating type information regulation 2homolog1)是一种具有NAD+依赖性去乙酰化酶活性的转录调节因子,参与能量代谢的调节。利用实时荧光定量PCR技术,对10月龄山西肉用绵羊母本品系去势公羊内脏(肝、肾、脾、肺、心)、肌肉...SIRT1(silent mating type information regulation 2homolog1)是一种具有NAD+依赖性去乙酰化酶活性的转录调节因子,参与能量代谢的调节。利用实时荧光定量PCR技术,对10月龄山西肉用绵羊母本品系去势公羊内脏(肝、肾、脾、肺、心)、肌肉(背最长肌)和脂肪(大网膜、小网膜、肠系膜、腹膜后、皮下)等11种器官或组织中SIRT1基因mRNA的表达量进行检测,以探讨SIRT1基因的差异性表达规律。结果表明,绵羊SIRT1基因在所检测的器官或组织中均有表达,且差异极显著(P<0.001)。主要表现在脾脏、肝脏和肾脏中的表达显著高于心脏和肺脏中的表达;深层脂肪组织中的表达高于浅层的皮下脂肪;在背最长肌中也有较高表达。这些差异与绵羊SIRT1基因调节脂质代谢及糖异生有关。有关结果为研究SIRT1基因的功能奠定了科学依据。展开更多
目的:脑白质高信号(white matter hyperintensity,WMH)是导致认知功能障碍的重要因素,机制目前仍未阐明。近年来研究发现环状RNA(circular RNA,circRNA)在脑血管疾病中存在差异性表达。本研究旨在分析circRNA在WMH伴认知功能障碍患者外...目的:脑白质高信号(white matter hyperintensity,WMH)是导致认知功能障碍的重要因素,机制目前仍未阐明。近年来研究发现环状RNA(circular RNA,circRNA)在脑血管疾病中存在差异性表达。本研究旨在分析circRNA在WMH伴认知功能障碍患者外周血单个核细胞中的表达谱,筛选其中差异性表达的circRNA,探讨circRNA在WMH伴认知功能障碍中的作用。方法:利用circRNA基因芯片检测WMH伴认知障碍者、WMH不伴认知障碍者及正常对照者(各纳入3例,男女比为2:1)外周血单个核细胞circRNA表达谱。筛选WMH伴认知障碍者的差异性表达circRNA,差异性表达circRNA的筛选标准为表达差异倍数变化(fold change,FC)≥2.0(|log_(2)FC≥1)且P<0.05。采用TargetScan和miRanda靶基因分析软件预测相关靶miRNA,Genespring软件预测靶基因。结果:与对照组比较,WMH伴认知障碍组显著上调的circRNA有5条,下调的有3条;WMH不伴认知障碍组显著上调的circRNA有8条,下调的有2条。WMH伴认知障碍组的circRNA表达谱分别与WMH不伴认知障碍组、对照组比较,未发现共同差异表达的circRNA;与对照组比较,WMH伴认知障碍组、WMH不伴认知障碍组的circRNA表达谱中hsa_circ_0092222均表达上调,hsa_circ_0000662和hsa_circ_0083773均表达下调,且它们在2组间的表达差异无统计学意义(均P>0.05)。预测到hsa_circ_0092222有2个靶miRNA(hsa-miR-19a-3p、hsa-miR-19b-3p),靶基因为核糖体蛋白S4,Y连锁1(ribosomal protein S4, Y-linked 1,RPS4Y1);hsa_circ_0000662有1个靶miRNA(hsa-miR-194),靶基因为轴抑制因子1(axis inhibitor 1, AXIN1);hsa_circ_0083773有7个靶miRNA,靶基因为重组清道夫受体A类成员3(recombinant scavenger receptor class A member 3,SCARA3)。结论:WMH患者的circRNA表达谱发生了显著变化;差异性表达的circRNA可能是导致脑白质出现高信号的原因;hsa_circ_0092222、hsa_circ_0000662、hsa_circ_0083773可能靶向吸附靶miRNA,调节靶基因的表达,通过Janus激酶2(Janus kinase 2,JAK2)/信号转导与转录活化因子3(signal transducers and activators of transcription,STAT3)信号通路、Wnt信号通路导致脑白质的损伤。未发现circRNA表达谱在WMH伴与不伴认知障碍患者中的显著差异,WMH患者出现认知障碍可能有其他发病机制。展开更多
文摘Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.
文摘SIRT1(silent mating type information regulation 2homolog1)是一种具有NAD+依赖性去乙酰化酶活性的转录调节因子,参与能量代谢的调节。利用实时荧光定量PCR技术,对10月龄山西肉用绵羊母本品系去势公羊内脏(肝、肾、脾、肺、心)、肌肉(背最长肌)和脂肪(大网膜、小网膜、肠系膜、腹膜后、皮下)等11种器官或组织中SIRT1基因mRNA的表达量进行检测,以探讨SIRT1基因的差异性表达规律。结果表明,绵羊SIRT1基因在所检测的器官或组织中均有表达,且差异极显著(P<0.001)。主要表现在脾脏、肝脏和肾脏中的表达显著高于心脏和肺脏中的表达;深层脂肪组织中的表达高于浅层的皮下脂肪;在背最长肌中也有较高表达。这些差异与绵羊SIRT1基因调节脂质代谢及糖异生有关。有关结果为研究SIRT1基因的功能奠定了科学依据。
文摘目的:脑白质高信号(white matter hyperintensity,WMH)是导致认知功能障碍的重要因素,机制目前仍未阐明。近年来研究发现环状RNA(circular RNA,circRNA)在脑血管疾病中存在差异性表达。本研究旨在分析circRNA在WMH伴认知功能障碍患者外周血单个核细胞中的表达谱,筛选其中差异性表达的circRNA,探讨circRNA在WMH伴认知功能障碍中的作用。方法:利用circRNA基因芯片检测WMH伴认知障碍者、WMH不伴认知障碍者及正常对照者(各纳入3例,男女比为2:1)外周血单个核细胞circRNA表达谱。筛选WMH伴认知障碍者的差异性表达circRNA,差异性表达circRNA的筛选标准为表达差异倍数变化(fold change,FC)≥2.0(|log_(2)FC≥1)且P<0.05。采用TargetScan和miRanda靶基因分析软件预测相关靶miRNA,Genespring软件预测靶基因。结果:与对照组比较,WMH伴认知障碍组显著上调的circRNA有5条,下调的有3条;WMH不伴认知障碍组显著上调的circRNA有8条,下调的有2条。WMH伴认知障碍组的circRNA表达谱分别与WMH不伴认知障碍组、对照组比较,未发现共同差异表达的circRNA;与对照组比较,WMH伴认知障碍组、WMH不伴认知障碍组的circRNA表达谱中hsa_circ_0092222均表达上调,hsa_circ_0000662和hsa_circ_0083773均表达下调,且它们在2组间的表达差异无统计学意义(均P>0.05)。预测到hsa_circ_0092222有2个靶miRNA(hsa-miR-19a-3p、hsa-miR-19b-3p),靶基因为核糖体蛋白S4,Y连锁1(ribosomal protein S4, Y-linked 1,RPS4Y1);hsa_circ_0000662有1个靶miRNA(hsa-miR-194),靶基因为轴抑制因子1(axis inhibitor 1, AXIN1);hsa_circ_0083773有7个靶miRNA,靶基因为重组清道夫受体A类成员3(recombinant scavenger receptor class A member 3,SCARA3)。结论:WMH患者的circRNA表达谱发生了显著变化;差异性表达的circRNA可能是导致脑白质出现高信号的原因;hsa_circ_0092222、hsa_circ_0000662、hsa_circ_0083773可能靶向吸附靶miRNA,调节靶基因的表达,通过Janus激酶2(Janus kinase 2,JAK2)/信号转导与转录活化因子3(signal transducers and activators of transcription,STAT3)信号通路、Wnt信号通路导致脑白质的损伤。未发现circRNA表达谱在WMH伴与不伴认知障碍患者中的显著差异,WMH患者出现认知障碍可能有其他发病机制。
