A microbial fuel cell(MFC)is a novel promising technology for simultaneous renewable electricity generation and wastewater treatment.Three non-comparable objectives,i.e.power density,attainable current density and was...A microbial fuel cell(MFC)is a novel promising technology for simultaneous renewable electricity generation and wastewater treatment.Three non-comparable objectives,i.e.power density,attainable current density and waste removal ratio,are often conflicting.A thorough understanding of the relationship among these three conflicting objectives can be greatly helpful to assist in optimal operation of MFC system.In this study,a multiobjective genetic algorithm is used to simultaneously maximizing power density,attainable current density and waste removal ratio based on a mathematical model for an acetate two-chamber MFC.Moreover,the level diagrams method is utilized to aid in graphical visualization of Pareto front and decision making.Three biobjective optimization problems and one three-objective optimization problem are thoroughly investigated.The obtained Pareto fronts illustrate the complex relationships among these three objectives,which is helpful for final decision support.Therefore,the integrated methodology of a multi-objective genetic algorithm and a graphical visualization technique provides a promising tool for the optimal operation of MFCs by simultaneously considering multiple conflicting objectives.展开更多
Two varieties of fiddleneck （Phacelia tanacetifolia Benth.） plant were determined for visualizing somatic chromosomes. The 4-5 days old root tips were pre-treated in 6% a-monobromonaphtalane in ＋4 ℃ for 7.5 h, the...Two varieties of fiddleneck （Phacelia tanacetifolia Benth.） plant were determined for visualizing somatic chromosomes. The 4-5 days old root tips were pre-treated in 6% a-monobromonaphtalane in ＋4 ℃ for 7.5 h, then fixed in glacial acetic acid for 30 min and transferred to 70% ethanol for long storage. When the root tips were analyzed, they were hydrolyzed with 1 N HCI for 13 min at room temperature （25 ℃）. After hydrolyzing, root tips were stained with 2% aceto orcein in darkness for 2.5 h. The squash method for preparation was used for chromosomal investigations. The chromosome length （C）, relative length （RL）, the long arm （L） and short arm （S） lengths, arm ratio （AR; L/S） and centromefic index （S/C） were calculated for caryologic parameters. The ideograms and detailed chromosome morphology measurements of the species were performed by the use of MicroMeasure 3.3. According to results, fiddleneck （Phacelia tanacetifolia Benth.） has 2n = 22 chromosomes and the karyotype formulas of two varieties of fiddleneck were 16 median and 6 submedian （16 m ＋ 6 sm）.展开更多
Animals communicate information within their environments via visual, chemical, auditory, and/or tactile modalities. The use of each modalityis generally linked to particular brain regions, but it is not yet known whe...Animals communicate information within their environments via visual, chemical, auditory, and/or tactile modalities. The use of each modalityis generally linked to particular brain regions, but it is not yet known whether the cellular morphology of neurons in these regions has evolved in association with the relative use of a modality.We investigated relationships between the behavioral use of communication modalities and neural morphologies in six lizard species. Two of these species （Anolis carol# nensis and Leiocephalus carinatus） primarily use visual signals to communicate with conspecifics and detect potential prey, and two （Aspidoscelis gularis and Scincella lateralis） communicate and forage primarily using chemical signals. Two other species （Hemidactylus turcicus and Sceloporus olivaceus） use both visual and chemical signals. For each species, we performed beha- vioral observations and quantified rates of visual and chemical behaviors. We then cryosectioned brain tissues from 9-10 males of each species and measured the soma size and density of neurons in two brain regions associated with visual behaviors （the lat- eral geniculate nucleus and the nucleus rotundus） and one region associated with chemical behaviors （the nucleus sphericus）. With analyses conducted in a phylogenetic context, we found that species that performed higher rates of visual displays had a denser lateral geniculate nucleus, and species that used a higher proportion of chemical displays had larger somas in the nucleus sphericus. These relationships suggest that neural morphologies in the brain have evolved convergently in species with similar communication behaviors .展开更多
基金Supported by the National Natural Science Foundation of China(21576163)the Major State Basic Research Development Program of China(2014CB239703)+1 种基金the Science and Technology Commission of Shanghai Municipality(14DZ2250800)the Project-sponsored by SRF for ROCS,SEM
文摘A microbial fuel cell(MFC)is a novel promising technology for simultaneous renewable electricity generation and wastewater treatment.Three non-comparable objectives,i.e.power density,attainable current density and waste removal ratio,are often conflicting.A thorough understanding of the relationship among these three conflicting objectives can be greatly helpful to assist in optimal operation of MFC system.In this study,a multiobjective genetic algorithm is used to simultaneously maximizing power density,attainable current density and waste removal ratio based on a mathematical model for an acetate two-chamber MFC.Moreover,the level diagrams method is utilized to aid in graphical visualization of Pareto front and decision making.Three biobjective optimization problems and one three-objective optimization problem are thoroughly investigated.The obtained Pareto fronts illustrate the complex relationships among these three objectives,which is helpful for final decision support.Therefore,the integrated methodology of a multi-objective genetic algorithm and a graphical visualization technique provides a promising tool for the optimal operation of MFCs by simultaneously considering multiple conflicting objectives.
文摘Two varieties of fiddleneck （Phacelia tanacetifolia Benth.） plant were determined for visualizing somatic chromosomes. The 4-5 days old root tips were pre-treated in 6% a-monobromonaphtalane in ＋4 ℃ for 7.5 h, then fixed in glacial acetic acid for 30 min and transferred to 70% ethanol for long storage. When the root tips were analyzed, they were hydrolyzed with 1 N HCI for 13 min at room temperature （25 ℃）. After hydrolyzing, root tips were stained with 2% aceto orcein in darkness for 2.5 h. The squash method for preparation was used for chromosomal investigations. The chromosome length （C）, relative length （RL）, the long arm （L） and short arm （S） lengths, arm ratio （AR; L/S） and centromefic index （S/C） were calculated for caryologic parameters. The ideograms and detailed chromosome morphology measurements of the species were performed by the use of MicroMeasure 3.3. According to results, fiddleneck （Phacelia tanacetifolia Benth.） has 2n = 22 chromosomes and the karyotype formulas of two varieties of fiddleneck were 16 median and 6 submedian （16 m ＋ 6 sm）.
基金We thank the members of the Johnson Lab at Trinity University for assistance in the field and labor- atory - particularly Bonnie Kircher, Alisa Dill, Michelle Ob- emdorf, Adam Silva, Taren Blackmon, Jordan Bush, McKen- zie Quinn, EUee Cook, Grayam Sailor-Tynes, and Collin Shinkle. We also thank David Ribble, Troy Murphy, Jimmy Roberts, Kevin McIntyre, and three reviewers for comments on an earlier version of this work, and Kevin McIntyre and Kimberly Phillips for statistical advice. This work was funded by a Sigma Xi GIAR （to C.D.R.）, a Trinity University Mind Science Foundation grant （to C.D.R.）, Trinity University＇s HHMI and Biology Summer Undergraduate Research Fel- lowships （to C.D.R.）, Texas Ecolab, and the National Science Foundation （IOS 1257021 to M.A.J.）. All procedures were approved by Trinity University＇s Animal Research Committee （protocol # 050213_MAJ2）.
文摘Animals communicate information within their environments via visual, chemical, auditory, and/or tactile modalities. The use of each modalityis generally linked to particular brain regions, but it is not yet known whether the cellular morphology of neurons in these regions has evolved in association with the relative use of a modality.We investigated relationships between the behavioral use of communication modalities and neural morphologies in six lizard species. Two of these species （Anolis carol# nensis and Leiocephalus carinatus） primarily use visual signals to communicate with conspecifics and detect potential prey, and two （Aspidoscelis gularis and Scincella lateralis） communicate and forage primarily using chemical signals. Two other species （Hemidactylus turcicus and Sceloporus olivaceus） use both visual and chemical signals. For each species, we performed beha- vioral observations and quantified rates of visual and chemical behaviors. We then cryosectioned brain tissues from 9-10 males of each species and measured the soma size and density of neurons in two brain regions associated with visual behaviors （the lat- eral geniculate nucleus and the nucleus rotundus） and one region associated with chemical behaviors （the nucleus sphericus）. With analyses conducted in a phylogenetic context, we found that species that performed higher rates of visual displays had a denser lateral geniculate nucleus, and species that used a higher proportion of chemical displays had larger somas in the nucleus sphericus. These relationships suggest that neural morphologies in the brain have evolved convergently in species with similar communication behaviors .
