Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS)...Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed under a phase contrast microscope and an electron microscope.Results: Observation under light microscopy and electron microscopy showed that high aggressive osteosarcoma cells line (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen in the absence of endothelial cells or fibroblasts.Conclusion: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry. Key words osteosarcoma cells line - vasculogenesis mimicry - angiogenesis - 3-dimensional cultures This study was supported in part by the National Natural Sciences Foundation of China (No. 30271314).展开更多
The chemical ingredients, morphological structure and properties of mulberry fiber have been researched. The results show that the mulberry fiber is cellulose fiber. The crystal structure of mulberry fiber is cellulos...The chemical ingredients, morphological structure and properties of mulberry fiber have been researched. The results show that the mulberry fiber is cellulose fiber. The crystal structure of mulberry fiber is cellulose I and the crystal cell belongs to the monoelinic system. The mulberry fiber crystal degree is 62.9% and orientation degree is 43.7%, the fineness of mulberry fiber is 2.3- 3.9 dtex, the length of the single fiber is 28 - 47 mm, the breaking strength of mulberry fiber is about 2.93 cN/dtex, and the initial modulus is about 76.82 cN/dtex.展开更多
基金This study was supported in part by the National Natural Sciences Foundation of China(No.30271314).
文摘Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed under a phase contrast microscope and an electron microscope.Results: Observation under light microscopy and electron microscopy showed that high aggressive osteosarcoma cells line (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen in the absence of endothelial cells or fibroblasts.Conclusion: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry. Key words osteosarcoma cells line - vasculogenesis mimicry - angiogenesis - 3-dimensional cultures This study was supported in part by the National Natural Sciences Foundation of China (No. 30271314).
基金Doctorate Foundation of Xi'an Polytechnic University and the 2006 Year Research Project of Shaanxi Educational Bureau , China (No.06JK284)
文摘The chemical ingredients, morphological structure and properties of mulberry fiber have been researched. The results show that the mulberry fiber is cellulose fiber. The crystal structure of mulberry fiber is cellulose I and the crystal cell belongs to the monoelinic system. The mulberry fiber crystal degree is 62.9% and orientation degree is 43.7%, the fineness of mulberry fiber is 2.3- 3.9 dtex, the length of the single fiber is 28 - 47 mm, the breaking strength of mulberry fiber is about 2.93 cN/dtex, and the initial modulus is about 76.82 cN/dtex.
