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微弹转化葡匐剪股颖使之抗除草剂
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作者 孙雷心 《生物技术通报》 CAS CSCD 1995年第4期17-18,共2页
Rutegers University的科学家们建立了一种可有效转化商品化葡匐剪股颖品种的系统。稳定整入并嵌合性表达bar(phosphinothricin乙酰转移酶)基因可使之获得对包括Herbiace在内的多种商品化除草荆的活性成份bialaphos的解毒能力。葡匐剪... Rutegers University的科学家们建立了一种可有效转化商品化葡匐剪股颖品种的系统。稳定整入并嵌合性表达bar(phosphinothricin乙酰转移酶)基因可使之获得对包括Herbiace在内的多种商品化除草荆的活性成份bialaphos的解毒能力。葡匐剪股颖在经多次刈割后可形成分枝细密、质量均一的优质草皮,这是一种用于高尔夫球场保绿的出色的耐冷品种,也可用于航道,高尔夫球场发球地和草坪。 曾有过通过体细胞胚发生作用再生葡匐剪股颖植株和用微弹轰击导入无选择标记的GUS(uidA)基因的报道。而在《Bio/Technology》1994年12期919~923页上叙述的这项研究。 展开更多
关键词 微弹转化 剪股颖 高尔夫球场 转基因植株 商品化 无选择标记 抗除草剂 体细胞胚 有效转化 轰击
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Genetic transformation of Pinus taeda by particle bombardment 被引量:1
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作者 唐巍 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第2期91-97,163,共7页
A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to... A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil. 展开更多
关键词 Pinus taeda L. Biolistic transformation Bacillus thuringiensis (B.t.) cryIAb Insect feeding bioassay
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Conifer genetic engineering: Particle bombardment and Agrobacterium-mediated gene transfer and its application in future forests 被引量:1
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作者 唐巍 《Journal of Forestry Research》 SCIE CAS CSCD 2001年第4期220-228,276,共10页
Many important advances in forest biotechnology have been made. The use of genetic transformation and the ap-plications of transgenic trees in modern forestry is now an important field. Two basic methodologies particl... Many important advances in forest biotechnology have been made. The use of genetic transformation and the ap-plications of transgenic trees in modern forestry is now an important field. Two basic methodologies particle bombardment and Agrobacterium-mediated transformation have been used on conifers. However, routine procedures exist for only a limited number of conifers. As a result only a few species have been successfully transformed into stable transgenic plants. The use of a particle bombardment has been more successful and transgenic plants have been produced in Picea abies, Picea glauca, Picea mariana, and Pinus radiata, although the level of production of stable transgenic plants is lower than that of Agrobacte-rium. At present, breeding programs have been directed toward improving bole shape, growth rate, wood properties, and quality, as well as toward improving root and shoot performance, pest resistance, stress tolerance, herbicide resistance, and ability to resist stresses, which will drive forestry to enter a new era of productivity and quality. This article provides a brief overview of the current state of knowledge on genetic transformation in conifers. 展开更多
关键词 Genetic engineering Particle bombardment AGROBACTERIUM CONIFER
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