尽管近年来中国氮(N)沉降水平逐渐趋于稳定,但中国东南地区N沉降相比于其他地区仍处于较高水平。N沉降对陆地生态系统碳循环过程的影响不容忽视。微生物碳利用效率(CUE)是指微生物将吸收的碳转化为生物量碳的效率,高微生物CUE意味着高...尽管近年来中国氮(N)沉降水平逐渐趋于稳定,但中国东南地区N沉降相比于其他地区仍处于较高水平。N沉降对陆地生态系统碳循环过程的影响不容忽视。微生物碳利用效率(CUE)是指微生物将吸收的碳转化为生物量碳的效率,高微生物CUE意味着高土壤有机碳存储潜力。因此,探究N沉降背景下微生物CUE的变化将有助于进一步认识陆地生态系统土壤碳存储的变化。然而,目前关于N沉降下微生物群落结构的变化如何影响微生物CUE鲜有报道。在福建省泉州市戴云山国家级自然保护区的罗浮栲林通过N添加模拟N沉降。实验共包括三个N添加处理:对照(CT,+0 kg hm^(-2)a^(-1))、低氮(LN,+40 kg hm^(-2)a^(-1))和高氮(HN,+80 kg hm^(-2)a^(-1))。测定不同处理土壤基本理化性质、微生物生物量、酶活性和CUE,并使用高通量测序对微生物群落结构和多样性进行测定。结果表明,N添加显著影响微生物CUE,随着N添加水平的增加,CUE逐渐增加;相反,土壤pH、可提取有机碳(EOC)和微生物生物量碳(MBC)均呈现下降趋势。N添加对土壤微生物群落α多样性总体上无显著影响。非度量多维度尺度(NMDS)分析表明,N添加显著改变了微生物的群落结构。尤其对于真菌而言,不同N添加处理的真菌群落明显分开为三簇。微生物CUE分别与土壤pH、EOC和真菌NMDS1呈显著的负相关关系,与矿质氮含量呈现显著正相关关系。随机森林分析表明,N添加下影响微生物CUE的类群主要是富营养菌(如变形菌门和子囊菌门)。研究表明N添加下,微生物CUE不仅受土壤养分有效性和pH的调控,同时还受土壤微生物群落结构的影响。未来进一步探究N添加下土壤微生物关键类群的变化可能有助于揭示森林生态系统碳存储过程。展开更多
作为调节土壤碳矿化过程的重要参数,微生物碳利用效率(CUE)对理解陆地生态系统中的碳循环至关重要。本研究在戴云山罗浮栲林设置对照(0 kg N·hm^(-2)·a^(-1))、低氮(40 kg N·hm^(-2)·a^(-1))和高氮(80 kg N·hm...作为调节土壤碳矿化过程的重要参数,微生物碳利用效率(CUE)对理解陆地生态系统中的碳循环至关重要。本研究在戴云山罗浮栲林设置对照(0 kg N·hm^(-2)·a^(-1))、低氮(40 kg N·hm^(-2)·a^(-1))和高氮(80 kg N·hm^(-2)·a^(-1))3个氮添加水平以模拟氮沉降,测定了表层(0~10 cm)土壤基本理化性质、有机碳组分、微生物生物量和酶活性;并利用^(18)O标记水方法测定土壤微生物CUE,以更好地理解氮沉降加剧对微生物CUE的影响及其影响因素。结果表明:短期氮添加显著降低了土壤微生物的呼吸速率、碳和氮获取酶活性,但显著增加了土壤微生物CUE。β-N-乙酰氨基酸葡糖苷酶(NAG)/微生物生物量碳(MBC)、微生物呼吸速率、β-葡萄糖苷酶(BG)/MBC、纤维素水解酶(CBH)/MBC和土壤有机碳含量是影响CUE的主要因素,且CUE与NAG/MBC、微生物呼吸速率、BG/MBC和CBH/MBC呈显著负相关,与土壤有机碳呈显著正相关。综上,短期氮添加导致土壤微生物获取碳和氮的成本降低,减少微生物呼吸,从而提高了土壤微生物CUE,这将有助于提高罗浮栲林土壤碳固存潜力。展开更多
The role of biophysical variables in constructing community structure changes with the time since fire.The major objective of this study is to verify the transition stage and its underlying variables for the postfire ...The role of biophysical variables in constructing community structure changes with the time since fire.The major objective of this study is to verify the transition stage and its underlying variables for the postfire forest and soil microbial function in the boreal forested area of China.A 50-year fire chronosequence was presented,and biomass of forbs,shrubs and woody plants was separately weighted to assess their contribution to the whole community with the year since fire(YSF).Simultaneously,soil biophysical properties were measured for stands in different time periods after fire.Soil microbial functions,i.e.growth efficiency(GE)and carbon use efficiency(CUE),were calculated based on ecoenzymatic and soil nutrient stoichiometry.In terms of vegetative structure,forbs’proportion decreased from 75%to 1.5%,but the proportion of woody plants increased from 0.04%to 70%across this fire chronosequence.GE and CUE of soil microorganisms averaged 0.242 and 0.236 and were significantly higher in 9,15 and 31 YSF than in 2 and 3 YSF.Soil metal content was significantly increased at the late stage of this fire chronosequence,and soil calcium content showed a positive correlation with woody plant biomass and a negative correlation with soil microbial function.Overall,the present work highlights that the time period of 15 and 31 YSF is a hallmark stage for aboveground vegetative structure and soil microbial function to change in different trends and that the calcium content may partly account for these two divergent trajectories.展开更多
文摘尽管近年来中国氮(N)沉降水平逐渐趋于稳定,但中国东南地区N沉降相比于其他地区仍处于较高水平。N沉降对陆地生态系统碳循环过程的影响不容忽视。微生物碳利用效率(CUE)是指微生物将吸收的碳转化为生物量碳的效率,高微生物CUE意味着高土壤有机碳存储潜力。因此,探究N沉降背景下微生物CUE的变化将有助于进一步认识陆地生态系统土壤碳存储的变化。然而,目前关于N沉降下微生物群落结构的变化如何影响微生物CUE鲜有报道。在福建省泉州市戴云山国家级自然保护区的罗浮栲林通过N添加模拟N沉降。实验共包括三个N添加处理:对照(CT,+0 kg hm^(-2)a^(-1))、低氮(LN,+40 kg hm^(-2)a^(-1))和高氮(HN,+80 kg hm^(-2)a^(-1))。测定不同处理土壤基本理化性质、微生物生物量、酶活性和CUE,并使用高通量测序对微生物群落结构和多样性进行测定。结果表明,N添加显著影响微生物CUE,随着N添加水平的增加,CUE逐渐增加;相反,土壤pH、可提取有机碳(EOC)和微生物生物量碳(MBC)均呈现下降趋势。N添加对土壤微生物群落α多样性总体上无显著影响。非度量多维度尺度(NMDS)分析表明,N添加显著改变了微生物的群落结构。尤其对于真菌而言,不同N添加处理的真菌群落明显分开为三簇。微生物CUE分别与土壤pH、EOC和真菌NMDS1呈显著的负相关关系,与矿质氮含量呈现显著正相关关系。随机森林分析表明,N添加下影响微生物CUE的类群主要是富营养菌(如变形菌门和子囊菌门)。研究表明N添加下,微生物CUE不仅受土壤养分有效性和pH的调控,同时还受土壤微生物群落结构的影响。未来进一步探究N添加下土壤微生物关键类群的变化可能有助于揭示森林生态系统碳存储过程。
文摘作为调节土壤碳矿化过程的重要参数,微生物碳利用效率(CUE)对理解陆地生态系统中的碳循环至关重要。本研究在戴云山罗浮栲林设置对照(0 kg N·hm^(-2)·a^(-1))、低氮(40 kg N·hm^(-2)·a^(-1))和高氮(80 kg N·hm^(-2)·a^(-1))3个氮添加水平以模拟氮沉降,测定了表层(0~10 cm)土壤基本理化性质、有机碳组分、微生物生物量和酶活性;并利用^(18)O标记水方法测定土壤微生物CUE,以更好地理解氮沉降加剧对微生物CUE的影响及其影响因素。结果表明:短期氮添加显著降低了土壤微生物的呼吸速率、碳和氮获取酶活性,但显著增加了土壤微生物CUE。β-N-乙酰氨基酸葡糖苷酶(NAG)/微生物生物量碳(MBC)、微生物呼吸速率、β-葡萄糖苷酶(BG)/MBC、纤维素水解酶(CBH)/MBC和土壤有机碳含量是影响CUE的主要因素,且CUE与NAG/MBC、微生物呼吸速率、BG/MBC和CBH/MBC呈显著负相关,与土壤有机碳呈显著正相关。综上,短期氮添加导致土壤微生物获取碳和氮的成本降低,减少微生物呼吸,从而提高了土壤微生物CUE,这将有助于提高罗浮栲林土壤碳固存潜力。
基金supported jointly by the Key Project of National Key Research and Development Plan(grant no.2017YFC0504002)the Fundamental Research Funds for the Central University(grant no.2015ZCQ-LX-03).
文摘The role of biophysical variables in constructing community structure changes with the time since fire.The major objective of this study is to verify the transition stage and its underlying variables for the postfire forest and soil microbial function in the boreal forested area of China.A 50-year fire chronosequence was presented,and biomass of forbs,shrubs and woody plants was separately weighted to assess their contribution to the whole community with the year since fire(YSF).Simultaneously,soil biophysical properties were measured for stands in different time periods after fire.Soil microbial functions,i.e.growth efficiency(GE)and carbon use efficiency(CUE),were calculated based on ecoenzymatic and soil nutrient stoichiometry.In terms of vegetative structure,forbs’proportion decreased from 75%to 1.5%,but the proportion of woody plants increased from 0.04%to 70%across this fire chronosequence.GE and CUE of soil microorganisms averaged 0.242 and 0.236 and were significantly higher in 9,15 and 31 YSF than in 2 and 3 YSF.Soil metal content was significantly increased at the late stage of this fire chronosequence,and soil calcium content showed a positive correlation with woody plant biomass and a negative correlation with soil microbial function.Overall,the present work highlights that the time period of 15 and 31 YSF is a hallmark stage for aboveground vegetative structure and soil microbial function to change in different trends and that the calcium content may partly account for these two divergent trajectories.
