The recent spring up of the antineoplastic agents and the prolonged survival bring both challenge and chance to radiological practice.Radiological methods including CT,MRI and PET play an increasingly important role i...The recent spring up of the antineoplastic agents and the prolonged survival bring both challenge and chance to radiological practice.Radiological methods including CT,MRI and PET play an increasingly important role in evaluating the efficacy of these antineoplastic drugs.However,different antineoplastic agents potentially induce different radiological signs,making it a challenge for radiological response evaluation,which depends mainly on one-sided morphological response evaluation criteria in solid tumors(RECIST)in the status quo of clinical practice.This brings opportunities for the development of radiomics,which is promising to serve as a surrogate for response evaluations of anti-tumor treatments.In this article,we introduce the basic concepts of radiomics,review the state-of-art radiomics researches with highlights of radiomics application in predictions of molecular biomarkers,treatment response,and prognosis.We also provide in-depth analyses on major obstacles and future direction of this new technique in clinical investigations on new antineoplastic agents.展开更多
AIM: To investigate the effect of bone marrow-derived monocytes transfected with RNA of CT-26 (a cell line of mouse colon carcinoma) on antitumor immunity. METHODS: Mouse bone marrow-derived monocytes were incubated w...AIM: To investigate the effect of bone marrow-derived monocytes transfected with RNA of CT-26 (a cell line of mouse colon carcinoma) on antitumor immunity. METHODS: Mouse bone marrow-derived monocytes were incubated with mouse granulocyte macrophage colony stimulating factor (mGM-CSF) in vitro, and the purity of monocytes was detected by flow cytometry. Total RNA of CT-26 was obtained by TRIzol's process, and monocytes were transfected by TransMessenger in vitro. The activity of cytotoxic T lymphocytes (CTL) in vivo was estimated by the modified lactate dehydrogenase (LDH) release assay. Changes of tumor size in mice and animal's survival time were observed in different groups. RESULTS: Monocytes from mouse bone marrow were successfully incubated, and the positive rate of CDllb was over 95%. Vaccination of the monocytes transfected with total RNA induced a high level of specific CTL activity in vivo, and made mice resistant to the subsequent challenge of parental tumor cells. In vivo effects induced by monocytes transfected with total RNA were stronger than those induced by monocytes pulsed with tumor cell lysates. CONCLUSION: Antigen presenting cells transfected with total RNA of CT-26 can present endogenous? tumor antigens, activate CTL, and effectively induce specific antitumor immunity.展开更多
Objective： To observe the influence of pH value on the proliferation of LAK cells and on the killing effect of rlL-2, IFN-α2b, TNF-α, LAK cells and doxorubicin on malignant tumor cells, and investigate the possibil...Objective： To observe the influence of pH value on the proliferation of LAK cells and on the killing effect of rlL-2, IFN-α2b, TNF-α, LAK cells and doxorubicin on malignant tumor cells, and investigate the possibility of increasing the efficacy of rlL-2 or IFN-α2b and doxorubicin by infusing sodium bicarbonate （NaHCO3） through target arteries. Methods： Separating single nucleus cells from peripheral blood of healthy men, and observing the influence of pH on the activation of single nucleus cells by rlL-2. MTT assay was used to measure the killing effect of rlL-2, IFN-α2b and TNF-α on 7404 cells and the increased effect of doxorubicin on rlL-2 and IFN-α2b, the cytotoxity of LAK cells in different pH. Forty-two patients with advanced primary liver cancer were obtained by stratified random, NaHCO3, rIL-2/IFN-α2b and doxorubicin were infused through target arteries. The efficacy was estimated after two cycles. Results： The conditions of pH 7.3 and pH 7.6 in vitro helped the proliferation of LAK cells and the killing effect of rIL-2, IFN-α2b and LAK cells on 7404 cells. In the condition of pH 6.8 there was almost no killing effect for LAK cells. In the condition of pH 7.0, 7.2, 7.4 and 7.6, the killing rate of TNF-α to 7404 cells increased by degrees, and in pH 7.4 the killing effect was the optimum. After two cycles treatments in the 42 patients with advanced primary liver cancer, the response rate （CR＋PR） was 88% （37/42）. The median overall response and median overall survival were increased, and no complication associated with infusing sodium bicarbonate was observed. Conclusion： The killing effect of rIL-2, IFN-α2b, TNF-α and doxorubicin on malignant tumor cells was enhanced by increasing the pH value.展开更多
In the present study, the zinc oxide nanoparticles(ZnO NPs) were prepared by using a sol-gel method. The characterization of ZnO NPs, such as particle size, morphology, crystal form, optical properties and p H-respons...In the present study, the zinc oxide nanoparticles(ZnO NPs) were prepared by using a sol-gel method. The characterization of ZnO NPs, such as particle size, morphology, crystal form, optical properties and p H-responsive behavior, was carried out. The in vitro anti-tumor activity of ZnO NPs was evaluated on PC-3M and 4T1 cell lines. The results indicated that ZnO NPs were spherical NPs with uniform particle size, excellent fluorescence properties, and p H-responsive behavior. The in vitro anti-tumor activity of ZnO NPs was observed on PC-3M and 4T1 cell lines. Considering to above characteristics, Zn O NPs could be used as drug delivery carries for loading active compound performing therapeutic and diagnostic effect.展开更多
文摘The recent spring up of the antineoplastic agents and the prolonged survival bring both challenge and chance to radiological practice.Radiological methods including CT,MRI and PET play an increasingly important role in evaluating the efficacy of these antineoplastic drugs.However,different antineoplastic agents potentially induce different radiological signs,making it a challenge for radiological response evaluation,which depends mainly on one-sided morphological response evaluation criteria in solid tumors(RECIST)in the status quo of clinical practice.This brings opportunities for the development of radiomics,which is promising to serve as a surrogate for response evaluations of anti-tumor treatments.In this article,we introduce the basic concepts of radiomics,review the state-of-art radiomics researches with highlights of radiomics application in predictions of molecular biomarkers,treatment response,and prognosis.We also provide in-depth analyses on major obstacles and future direction of this new technique in clinical investigations on new antineoplastic agents.
文摘AIM: To investigate the effect of bone marrow-derived monocytes transfected with RNA of CT-26 (a cell line of mouse colon carcinoma) on antitumor immunity. METHODS: Mouse bone marrow-derived monocytes were incubated with mouse granulocyte macrophage colony stimulating factor (mGM-CSF) in vitro, and the purity of monocytes was detected by flow cytometry. Total RNA of CT-26 was obtained by TRIzol's process, and monocytes were transfected by TransMessenger in vitro. The activity of cytotoxic T lymphocytes (CTL) in vivo was estimated by the modified lactate dehydrogenase (LDH) release assay. Changes of tumor size in mice and animal's survival time were observed in different groups. RESULTS: Monocytes from mouse bone marrow were successfully incubated, and the positive rate of CDllb was over 95%. Vaccination of the monocytes transfected with total RNA induced a high level of specific CTL activity in vivo, and made mice resistant to the subsequent challenge of parental tumor cells. In vivo effects induced by monocytes transfected with total RNA were stronger than those induced by monocytes pulsed with tumor cell lysates. CONCLUSION: Antigen presenting cells transfected with total RNA of CT-26 can present endogenous? tumor antigens, activate CTL, and effectively induce specific antitumor immunity.
基金a grant from the Key Scientific Research Projects of Anhui Province (No. 0123027).
文摘Objective： To observe the influence of pH value on the proliferation of LAK cells and on the killing effect of rlL-2, IFN-α2b, TNF-α, LAK cells and doxorubicin on malignant tumor cells, and investigate the possibility of increasing the efficacy of rlL-2 or IFN-α2b and doxorubicin by infusing sodium bicarbonate （NaHCO3） through target arteries. Methods： Separating single nucleus cells from peripheral blood of healthy men, and observing the influence of pH on the activation of single nucleus cells by rlL-2. MTT assay was used to measure the killing effect of rlL-2, IFN-α2b and TNF-α on 7404 cells and the increased effect of doxorubicin on rlL-2 and IFN-α2b, the cytotoxity of LAK cells in different pH. Forty-two patients with advanced primary liver cancer were obtained by stratified random, NaHCO3, rIL-2/IFN-α2b and doxorubicin were infused through target arteries. The efficacy was estimated after two cycles. Results： The conditions of pH 7.3 and pH 7.6 in vitro helped the proliferation of LAK cells and the killing effect of rIL-2, IFN-α2b and LAK cells on 7404 cells. In the condition of pH 6.8 there was almost no killing effect for LAK cells. In the condition of pH 7.0, 7.2, 7.4 and 7.6, the killing rate of TNF-α to 7404 cells increased by degrees, and in pH 7.4 the killing effect was the optimum. After two cycles treatments in the 42 patients with advanced primary liver cancer, the response rate （CR＋PR） was 88% （37/42）. The median overall response and median overall survival were increased, and no complication associated with infusing sodium bicarbonate was observed. Conclusion： The killing effect of rIL-2, IFN-α2b, TNF-α and doxorubicin on malignant tumor cells was enhanced by increasing the pH value.
基金National Natural Science Foundation of China(Grant No.81773646)the National Key Research and Development Program of China(Grant No.2017YFA0205600)the Innovation Team of the Ministry of Education(Grant No.BMU2017TD003)。
文摘In the present study, the zinc oxide nanoparticles(ZnO NPs) were prepared by using a sol-gel method. The characterization of ZnO NPs, such as particle size, morphology, crystal form, optical properties and p H-responsive behavior, was carried out. The in vitro anti-tumor activity of ZnO NPs was evaluated on PC-3M and 4T1 cell lines. The results indicated that ZnO NPs were spherical NPs with uniform particle size, excellent fluorescence properties, and p H-responsive behavior. The in vitro anti-tumor activity of ZnO NPs was observed on PC-3M and 4T1 cell lines. Considering to above characteristics, Zn O NPs could be used as drug delivery carries for loading active compound performing therapeutic and diagnostic effect.
