Twelve known compounds, colchicine, 2-demethylcolchicine, 2-demethyldemecolcine, 2-demethylcolchifoline, 2-demethyl-lumicolchicine, lumicolchicine, demecolcine, luteolin, apigenin, n-hentriacontane, n-triacontanol and...Twelve known compounds, colchicine, 2-demethylcolchicine, 2-demethyldemecolcine, 2-demethylcolchifoline, 2-demethyl-lumicolchicine, lumicolchicine, demecolcine, luteolin, apigenin, n-hentriacontane, n-triacontanol and sitosterol were isolated from cormsand flowers of colchicum autumnale Liliaceae which was introduced from Europe and cultivated in Zhaotong Yunnan, China The contents ofcolchicine in corms and flowers of colchicum autumnale were 0.015% and 0.04% respectively, and the content of demecolcine in corms was0.043%.展开更多
文摘根据水仙黄条病毒、水仙退化病毒和水仙花叶病毒外壳蛋白(CP)基因的保守区序列设计3对特异性引物,通过优化包括Mg2+浓度、c DNA浓度、Taq DNA聚合酶浓度等在内的反应条件,建立了能同时检测这3种病毒的多重RT-PCR检测体系,并与单一RT-PCR做了比较.应用该体系能成功对3种病毒混合侵染的水仙样品进行多重RT-PCR扩增,获得751、623和483 bp 3条与预期大小相符的特异性条带,且RNA稀释至10-4倍时仍能被检测到.建立的多重RT-PCR检测体系具有良好的特异性和较高的灵敏度,比单一RT-PCR扩增更加方便、快速.
文摘Twelve known compounds, colchicine, 2-demethylcolchicine, 2-demethyldemecolcine, 2-demethylcolchifoline, 2-demethyl-lumicolchicine, lumicolchicine, demecolcine, luteolin, apigenin, n-hentriacontane, n-triacontanol and sitosterol were isolated from cormsand flowers of colchicum autumnale Liliaceae which was introduced from Europe and cultivated in Zhaotong Yunnan, China The contents ofcolchicine in corms and flowers of colchicum autumnale were 0.015% and 0.04% respectively, and the content of demecolcine in corms was0.043%.
