To investigate the effects of Tianshifu soil conditioners on rice growth and their control effects on Cd absorption and accumulation in rice, a test was carded out in the field typically polluted by Cd in Hunan Provin...To investigate the effects of Tianshifu soil conditioners on rice growth and their control effects on Cd absorption and accumulation in rice, a test was carded out in the field typically polluted by Cd in Hunan Province. The results showed that the Tianshifu soil conditioner, soil bacterial conditioner and the combination of Tian- shifu soil conditioner and soil bacterial conditioner all could promote rice growth, im- prove rice yield and reduce significantly Cd content in rice grains. In Beishan, the Cd contents in rice grains treated by Tianshifu soil conditioner, soil bacterial condi- tioner and the combination of Tianshifu soil conditioner and soil bacterial conditioner were reduced by 37.63% （P〈0.05）, 32.59% （P〈0.05） and 27.95% （P〈0.05） respec- tively compared with that in rice grains treated by conventional fertilization; In Xiang- tan, the Cd contents in rice grains were reduced by 54.36% （P〈0.05）, 50.19% （P〈 0.05） and 45.22% （P〈0.05） respectively. The rice grains harvested at the two test sites all met the national food safety standards. Considering the control effect on Cd contamination, Tianshifu soil conditioner 〉 combination of Tianshifu soil condi- tioner and soil bacterial conditioner 〉 Tianshifu soil bacterial conditioner. In addition to reducing Cd absorption in rice, as described in the lime application treatment, the application of Tianshifu soil conditioners also inhibited the transportation of Cd from rice straws to rice grains. The Tianshifu soil conditioner, soil bacterial conditioner and combination of Tianshifu soil conditioner and soil bacterial conditioner also effec- tively improved the soil acidity, increased the soil pH value and reduced soil avail- able Cd content to some extent.展开更多
Genotypic and environmental variation in Cd, Cr, As, Ni and Pb concentrations of grains, and the relationships between these heavy metals and Fe, Zn were investigated using 9 rice genotypes grown in 6 locations for tw...Genotypic and environmental variation in Cd, Cr, As, Ni and Pb concentrations of grains, and the relationships between these heavy metals and Fe, Zn were investigated using 9 rice genotypes grown in 6 locations for two successive years. Significant genotypic variation was detected in the five heavy metal concentrations in grains, indicating the possibility to reduce the concentration of these heavy metals in grains through breeding approach. The environmental effect varied with metal, with Pb and Ni having greater variation than the other three metals. There was significant genotype-environment (location) interaction of the concentrations of all five heavy metals in grains, suggesting the importance of cultivar choice in producing rice with low heavy metal concentrations in grains for a given location. Correlation analysis showed that Cd and As, Cr and Ni, and As and Pb con-centrations in rice grains were closely associated, and that Ni concentration in grains was negatively correlated with Zn concen-tration.展开更多
Effects of different lead compounds, PbCl2, Pb(NO3)2 and Pb(OAc)2, on the rice growth and uptake of lead and some microelements by wetland rice were studied. The results showed that the seed germination, rice seedling...Effects of different lead compounds, PbCl2, Pb(NO3)2 and Pb(OAc)2, on the rice growth and uptake of lead and some microelements by wetland rice were studied. The results showed that the seed germination, rice seedling growth, chlorophyl content, grain yield and uptake of Pb, Cu, Zn, Fe and Mn by rice plant were affected by the chemical forms of Pb compounds added in soil to a certain degree. The germination rate and the amount of chlorophyl decreased remarkably with increasing Pb concentration, the root extension was restrained obviously by the presence of Pb, and the effect of PbCl2 was more evident than that of Pb(NO3)2 or Pb(OAc)2. The pot incubation test with yellow brown soil and red soil showed that there was no significant regularity in effect of Pb on grain yield, but the difference in the influence of various Pb compounds on yield was clearer. The effect on the amount of Pb in straw and brown rice was in the sequence of Pb(NO3)2> Pb(OAc)2> PbCl2. In case the content of Pb in brown rice was 0.5 mg/ kg, the relative loading capacities of yellow brown soil for Pb added as PbCl2, Pb(OAc)2 and Pb(NO3)2 were 100, 90 and 60 respectively. Pb uptake by wetland rice was closely related to the chemical species of Pb in soil, but there was no comparability among chemical forms of different Pb compounds in the same soil. The uptake of Cu, Zn, Fe and Mn by wetland rice was markedly affected by the addition of Pb, and different Pb compounds varied in their impacts on the uptake of other metals by different organs of wetland rice, e.g. the concentration of Fe in root increased significantly (r = 0.92), while opposite was true for Fe in brown rice (r =-0.92) due to the application of Pb(OAc), in soil. These results demonstrate that the effect of accompanying anions of Pb on the physiological and biochemical processes of wetland rice was rather complex.展开更多
In the light of the national policy of fallow, this study was conducted to determine how the different water management and lime application would affect soil physical and chemical properties, rice yield and cadmium ...In the light of the national policy of fallow, this study was conducted to determine how the different water management and lime application would affect soil physical and chemical properties, rice yield and cadmium （Cd） content of rice in fallow season. The results showed that, compared with the arid fallow, the waterlogging fallow decreased the soil pH value whereas signifcantly increased the soil organic matter content and the cation exchange quantity, and reduced the soil effective cadmium content and the rice cadmium content whereas could increase the rice yield to a certain extent. In the fooded fallow or the dry fallow, the application of lime mainly depended on the alkali conditioning of lime and the antagonistic effect of Ca2＋, which could signifcantly reduce the cadmium content of rice, and its effect would increase linearly with the increase of lime dosage, whereas had no significant effect on soil organic matter content and cation exchange quantity. In order to establish a linear equation of lime dosage and related indexes under the condition of waterlogging fallow or dry fallow, calculations showed that each application of lime at 1 000 kg/hm2 or kg/hmss2 could improve soil pH value by 0.238 2 or 0.246 5units respectively, and reduce the effective Cd content to 0.007 5 mg/kg both in the arid fallow and the waterlogging fallow conditions. The lime theoretic application rate for the lowest Cd content of late rice in the arid fallow was 5 120 kg/hm2, and the minimum limit of the Cd content in rice was 0.124 2 mg/kg; and the lime theoretic application rate for the highest yield of late rice in the submerged water fallow was 4 636 kg/hm2, the minimum theoretic Cd content in rice is 0.100 7 mg/kg, and it could reduce the Cd content in rice under the condition of submerged fallow and decrease the dosage of lime.展开更多
Of the factors affecting migration and bioavailability of toxic metals in heavy metal contaminated soil, DOC （dissolved organic carbon） provides binding sites for metal cations and reduces the fixation and adsorptio...Of the factors affecting migration and bioavailability of toxic metals in heavy metal contaminated soil, DOC （dissolved organic carbon） provides binding sites for metal cations and reduces the fixation and adsorption of heavy metals by soil solid phase. Elevation of DOC level due to the direct incorporation of crop residues may lead to enhanced accumulation of toxic metals in crop body grown in polluted farmland. In this study, an incubation experiment and a pot experiment were conducted respectively to investigate the effects of wheat straw incorporation on DOC level, cadmium availability, and Cd accumulation in rice plant, and to establish the relation between Cd solubility and DOC level. A Cd-contaminated rice soil was used and incorporated with different rates （0%, 0.5% and 1%） of wheat straw in both experiments. Results showed that the change pattern of Cd in soil solution was very similar to that of DOC level. Wheat straw addition significantly elevated Cd and DOC level in soil solution while NH4NO3-extrated Cd was not affected. There existed a close linear correlation between soluble Cd and DOC level. Enhanced Cd accumulation in rice plant, grown in a Cd contaminated soil, induced by wheat straw incorporation was observed in this study.展开更多
There is an increasing concern about rice （Oryza sativa L.） soil microbiomes under the influence of mixed heavy metal contamina- tion. We used the high-throughput Illumina MiSeq sequencing approach to explore the ba...There is an increasing concern about rice （Oryza sativa L.） soil microbiomes under the influence of mixed heavy metal contamina- tion. We used the high-throughput Illumina MiSeq sequencing approach to explore the bacterial diversity and community composition of soils in four paddy fields, exhibiting four degrees of mixed heavy metal （Cd, Pb and Zn） pollution, and examined the effects of these metals on the bacterial communities. Our results showed that up to 2 104 to 4 359 bacterial operational taxonomic units （OTUs） were found in the bulk and rhizosphere soils of the paddy fields, with the dominant bacterial phyla （greater than 1% of the overall community） including Proteobacteria, Actinobacteria, Firmicutes, Acidobacteria, Gemmatimonadetes, Chlorofiexi, Bacteroidetes and Nitrospirem. A number of rare and candidate bacterial groups were also detected, and Saprospirales, HOC36, SC-I-84 and Anaerospora were rarely detected in rice paddy soils. Venn diagram analysis showed that 174 bacterial OTUs were shared among the bulk soils with four pollution degrees. Rice rhizosphere soils displayed higher bacterial diversity indices （ACE and Chao 1） and more unique OTUs than bulk soils. Total Cd and Zn in the soils were significantly negatively correlated with ACE and Chao 1, respectively, and the Mantel test suggested that total Pb, total Zn, pH, total nitrogen and total phosphorus significantly affected the community structure. Overall, these results provided baseline data for the bacterial communities in bulk and rhizosphere soils of paddy fields contaminated with mixed heavy metals.展开更多
文摘To investigate the effects of Tianshifu soil conditioners on rice growth and their control effects on Cd absorption and accumulation in rice, a test was carded out in the field typically polluted by Cd in Hunan Province. The results showed that the Tianshifu soil conditioner, soil bacterial conditioner and the combination of Tian- shifu soil conditioner and soil bacterial conditioner all could promote rice growth, im- prove rice yield and reduce significantly Cd content in rice grains. In Beishan, the Cd contents in rice grains treated by Tianshifu soil conditioner, soil bacterial condi- tioner and the combination of Tianshifu soil conditioner and soil bacterial conditioner were reduced by 37.63% （P〈0.05）, 32.59% （P〈0.05） and 27.95% （P〈0.05） respec- tively compared with that in rice grains treated by conventional fertilization; In Xiang- tan, the Cd contents in rice grains were reduced by 54.36% （P〈0.05）, 50.19% （P〈 0.05） and 45.22% （P〈0.05） respectively. The rice grains harvested at the two test sites all met the national food safety standards. Considering the control effect on Cd contamination, Tianshifu soil conditioner 〉 combination of Tianshifu soil condi- tioner and soil bacterial conditioner 〉 Tianshifu soil bacterial conditioner. In addition to reducing Cd absorption in rice, as described in the lime application treatment, the application of Tianshifu soil conditioners also inhibited the transportation of Cd from rice straws to rice grains. The Tianshifu soil conditioner, soil bacterial conditioner and combination of Tianshifu soil conditioner and soil bacterial conditioner also effec- tively improved the soil acidity, increased the soil pH value and reduced soil avail- able Cd content to some extent.
基金Project supported by the Postdoctoral Science Foundation of China(No. 2005037824), the Natural Science Foundation of ZhejiangProvince (No. Z304104), China, the Science and Technology De-partment of Zhejiang Province (No. 2005C32007), China, and theScience and Technology Bureau of Jiaxing, Zhejiang Province (No.2005AZ3003), China
文摘Genotypic and environmental variation in Cd, Cr, As, Ni and Pb concentrations of grains, and the relationships between these heavy metals and Fe, Zn were investigated using 9 rice genotypes grown in 6 locations for two successive years. Significant genotypic variation was detected in the five heavy metal concentrations in grains, indicating the possibility to reduce the concentration of these heavy metals in grains through breeding approach. The environmental effect varied with metal, with Pb and Ni having greater variation than the other three metals. There was significant genotype-environment (location) interaction of the concentrations of all five heavy metals in grains, suggesting the importance of cultivar choice in producing rice with low heavy metal concentrations in grains for a given location. Correlation analysis showed that Cd and As, Cr and Ni, and As and Pb con-centrations in rice grains were closely associated, and that Ni concentration in grains was negatively correlated with Zn concen-tration.
文摘Effects of different lead compounds, PbCl2, Pb(NO3)2 and Pb(OAc)2, on the rice growth and uptake of lead and some microelements by wetland rice were studied. The results showed that the seed germination, rice seedling growth, chlorophyl content, grain yield and uptake of Pb, Cu, Zn, Fe and Mn by rice plant were affected by the chemical forms of Pb compounds added in soil to a certain degree. The germination rate and the amount of chlorophyl decreased remarkably with increasing Pb concentration, the root extension was restrained obviously by the presence of Pb, and the effect of PbCl2 was more evident than that of Pb(NO3)2 or Pb(OAc)2. The pot incubation test with yellow brown soil and red soil showed that there was no significant regularity in effect of Pb on grain yield, but the difference in the influence of various Pb compounds on yield was clearer. The effect on the amount of Pb in straw and brown rice was in the sequence of Pb(NO3)2> Pb(OAc)2> PbCl2. In case the content of Pb in brown rice was 0.5 mg/ kg, the relative loading capacities of yellow brown soil for Pb added as PbCl2, Pb(OAc)2 and Pb(NO3)2 were 100, 90 and 60 respectively. Pb uptake by wetland rice was closely related to the chemical species of Pb in soil, but there was no comparability among chemical forms of different Pb compounds in the same soil. The uptake of Cu, Zn, Fe and Mn by wetland rice was markedly affected by the addition of Pb, and different Pb compounds varied in their impacts on the uptake of other metals by different organs of wetland rice, e.g. the concentration of Fe in root increased significantly (r = 0.92), while opposite was true for Fe in brown rice (r =-0.92) due to the application of Pb(OAc), in soil. These results demonstrate that the effect of accompanying anions of Pb on the physiological and biochemical processes of wetland rice was rather complex.
文摘In the light of the national policy of fallow, this study was conducted to determine how the different water management and lime application would affect soil physical and chemical properties, rice yield and cadmium （Cd） content of rice in fallow season. The results showed that, compared with the arid fallow, the waterlogging fallow decreased the soil pH value whereas signifcantly increased the soil organic matter content and the cation exchange quantity, and reduced the soil effective cadmium content and the rice cadmium content whereas could increase the rice yield to a certain extent. In the fooded fallow or the dry fallow, the application of lime mainly depended on the alkali conditioning of lime and the antagonistic effect of Ca2＋, which could signifcantly reduce the cadmium content of rice, and its effect would increase linearly with the increase of lime dosage, whereas had no significant effect on soil organic matter content and cation exchange quantity. In order to establish a linear equation of lime dosage and related indexes under the condition of waterlogging fallow or dry fallow, calculations showed that each application of lime at 1 000 kg/hm2 or kg/hmss2 could improve soil pH value by 0.238 2 or 0.246 5units respectively, and reduce the effective Cd content to 0.007 5 mg/kg both in the arid fallow and the waterlogging fallow conditions. The lime theoretic application rate for the lowest Cd content of late rice in the arid fallow was 5 120 kg/hm2, and the minimum limit of the Cd content in rice was 0.124 2 mg/kg; and the lime theoretic application rate for the highest yield of late rice in the submerged water fallow was 4 636 kg/hm2, the minimum theoretic Cd content in rice is 0.100 7 mg/kg, and it could reduce the Cd content in rice under the condition of submerged fallow and decrease the dosage of lime.
文摘Of the factors affecting migration and bioavailability of toxic metals in heavy metal contaminated soil, DOC （dissolved organic carbon） provides binding sites for metal cations and reduces the fixation and adsorption of heavy metals by soil solid phase. Elevation of DOC level due to the direct incorporation of crop residues may lead to enhanced accumulation of toxic metals in crop body grown in polluted farmland. In this study, an incubation experiment and a pot experiment were conducted respectively to investigate the effects of wheat straw incorporation on DOC level, cadmium availability, and Cd accumulation in rice plant, and to establish the relation between Cd solubility and DOC level. A Cd-contaminated rice soil was used and incorporated with different rates （0%, 0.5% and 1%） of wheat straw in both experiments. Results showed that the change pattern of Cd in soil solution was very similar to that of DOC level. Wheat straw addition significantly elevated Cd and DOC level in soil solution while NH4NO3-extrated Cd was not affected. There existed a close linear correlation between soluble Cd and DOC level. Enhanced Cd accumulation in rice plant, grown in a Cd contaminated soil, induced by wheat straw incorporation was observed in this study.
基金supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (No. 28100014)the Research and Development Office of the Education University of Hong Kong, China (No. RG84/2012-2013R)the NSFC-Guangdong United Foundation, China (No. U1501232)
文摘There is an increasing concern about rice （Oryza sativa L.） soil microbiomes under the influence of mixed heavy metal contamina- tion. We used the high-throughput Illumina MiSeq sequencing approach to explore the bacterial diversity and community composition of soils in four paddy fields, exhibiting four degrees of mixed heavy metal （Cd, Pb and Zn） pollution, and examined the effects of these metals on the bacterial communities. Our results showed that up to 2 104 to 4 359 bacterial operational taxonomic units （OTUs） were found in the bulk and rhizosphere soils of the paddy fields, with the dominant bacterial phyla （greater than 1% of the overall community） including Proteobacteria, Actinobacteria, Firmicutes, Acidobacteria, Gemmatimonadetes, Chlorofiexi, Bacteroidetes and Nitrospirem. A number of rare and candidate bacterial groups were also detected, and Saprospirales, HOC36, SC-I-84 and Anaerospora were rarely detected in rice paddy soils. Venn diagram analysis showed that 174 bacterial OTUs were shared among the bulk soils with four pollution degrees. Rice rhizosphere soils displayed higher bacterial diversity indices （ACE and Chao 1） and more unique OTUs than bulk soils. Total Cd and Zn in the soils were significantly negatively correlated with ACE and Chao 1, respectively, and the Mantel test suggested that total Pb, total Zn, pH, total nitrogen and total phosphorus significantly affected the community structure. Overall, these results provided baseline data for the bacterial communities in bulk and rhizosphere soils of paddy fields contaminated with mixed heavy metals.
