[Objective] The aim of this study was to investigate the dry matter accumulation,moisture content in maize kernel and their influences on mechanical harvesting.[Methods] Using Zhengdan 958,Xianyu 335,Hongda 8 and Liyu...[Objective] The aim of this study was to investigate the dry matter accumulation,moisture content in maize kernel and their influences on mechanical harvesting.[Methods] Using Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 as experimental materials,we preliminarily measured the dynamic changes of dry matter accumulation and moisture content with the days after pollination lasted,and investigated the effect of moisture content in kernels on mechanical harvesting of Zhengdan 958 and Liyu 16.[Results] During summer growing season,Zhengdan 958 and Liyu 16 became physiologically mature and suitable for harvesting at about 51 days after pollination,Xianyu 335 and Hongda 8 required 58 days to become physiologically mature and suitable for harvesting after pollination.At physiologically mature stage,the moisture contents in kernels of Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 were respectively 33.74%,28.86%,32.05% and 35.24%,respectively.Adopting mechanical harvesting at physiologically mature stage,the kernel loss rate of Zhengdan 958 and Liyu 16 was consistent with the range of mechanical operation index(kernel loss rate of 0.84%≤2%),while the percentage of broken kernels did not accord with the range of mechanical operation index(kernel crashing rate of 10.12%≧1%).The results indicate that Zhengdan 958 and Liyu 16 are not suitable for mechanical harvesting using full feeding combine havester.[Conclusion] This study provided theoretical basis for improving the maize harvest index under delayed harvesting.展开更多
Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegr...Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.展开更多
[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiati...[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong'erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.展开更多
The changes of physiological and biochemical indices in jujube fruits during the late development were investigated from 6-year-old jujube trees (Zizyphus jujuba Mill. cv. Lingwuchangzao). The results showed that th...The changes of physiological and biochemical indices in jujube fruits during the late development were investigated from 6-year-old jujube trees (Zizyphus jujuba Mill. cv. Lingwuchangzao). The results showed that the flesh firmness decreased slowly from white-green stage to full-red stage, being significantly related with the developmental maturity of jujube fruits negatively, the correlation coefficient reached -0.980 3^**. The contents of ascorbic acid and titratable acid in jujube fruits were significantly related with the developmental process of jujube fruits negatively or positively, the correlation coef- ficients were -0. 973 1^**and + 0. 974 6^ * * , respectively. The contents of soluble solids, total sugar, and sucrose increased with jujube ripening, while the relative sweetness of jujube fruits showed the same variation pattern, the correlation coefficients were 0. 996 6 ^* * , 0. 988 0^** , and 0. 982 8 ^* * , respectively. Before white-green stage during fruit development, the accumulation of monosaccharide was predominant in jujube fruits, following a fast accumulation of sucrose, indicating that the main component of sugars is sucrose at the crisp-ripe stage. Furthermore, the starch content of the flesh reached the peak at about thirty percentage of jujube maturity, being 51,54 mg/100 g.FW. The respiratory rates varied between 10 mg/( kg.h) and CO2 26 mg/( kg.h) after fruit turning red and before softening, indicating a non-climacteric respiratory type.展开更多
基金Supported by Supporting Program for Sci & Tech Research of China(2009BADA6B01)Natural Science Foundation of Anhui Province(090411017)~~
文摘[Objective] The aim of this study was to investigate the dry matter accumulation,moisture content in maize kernel and their influences on mechanical harvesting.[Methods] Using Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 as experimental materials,we preliminarily measured the dynamic changes of dry matter accumulation and moisture content with the days after pollination lasted,and investigated the effect of moisture content in kernels on mechanical harvesting of Zhengdan 958 and Liyu 16.[Results] During summer growing season,Zhengdan 958 and Liyu 16 became physiologically mature and suitable for harvesting at about 51 days after pollination,Xianyu 335 and Hongda 8 required 58 days to become physiologically mature and suitable for harvesting after pollination.At physiologically mature stage,the moisture contents in kernels of Zhengdan 958,Xianyu 335,Hongda 8 and Liyu 16 were respectively 33.74%,28.86%,32.05% and 35.24%,respectively.Adopting mechanical harvesting at physiologically mature stage,the kernel loss rate of Zhengdan 958 and Liyu 16 was consistent with the range of mechanical operation index(kernel loss rate of 0.84%≤2%),while the percentage of broken kernels did not accord with the range of mechanical operation index(kernel crashing rate of 10.12%≧1%).The results indicate that Zhengdan 958 and Liyu 16 are not suitable for mechanical harvesting using full feeding combine havester.[Conclusion] This study provided theoretical basis for improving the maize harvest index under delayed harvesting.
基金Supported by National Natural Science Foundation of China(30471274)~~
文摘Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.
基金Supported by the National Natural Science Foundation (30571049)~~
文摘[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong'erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.
基金Natural Science Foundation of Ningxia Hui Autonomous Region(B1007)~~
文摘The changes of physiological and biochemical indices in jujube fruits during the late development were investigated from 6-year-old jujube trees (Zizyphus jujuba Mill. cv. Lingwuchangzao). The results showed that the flesh firmness decreased slowly from white-green stage to full-red stage, being significantly related with the developmental maturity of jujube fruits negatively, the correlation coefficient reached -0.980 3^**. The contents of ascorbic acid and titratable acid in jujube fruits were significantly related with the developmental process of jujube fruits negatively or positively, the correlation coef- ficients were -0. 973 1^**and + 0. 974 6^ * * , respectively. The contents of soluble solids, total sugar, and sucrose increased with jujube ripening, while the relative sweetness of jujube fruits showed the same variation pattern, the correlation coefficients were 0. 996 6 ^* * , 0. 988 0^** , and 0. 982 8 ^* * , respectively. Before white-green stage during fruit development, the accumulation of monosaccharide was predominant in jujube fruits, following a fast accumulation of sucrose, indicating that the main component of sugars is sucrose at the crisp-ripe stage. Furthermore, the starch content of the flesh reached the peak at about thirty percentage of jujube maturity, being 51,54 mg/100 g.FW. The respiratory rates varied between 10 mg/( kg.h) and CO2 26 mg/( kg.h) after fruit turning red and before softening, indicating a non-climacteric respiratory type.