Two hydrocarbon degrading bacteria NJ276 and NJ341 were screened from 385 Antarctic marine bacteria and their degrading characteristics were studied. Diesel oil as sole carbon source was used in this study. The result...Two hydrocarbon degrading bacteria NJ276 and NJ341 were screened from 385 Antarctic marine bacteria and their degrading characteristics were studied. Diesel oil as sole carbon source was used in this study. The results showed that the oil degradation rates of Antarctic psychrophile bacteria NJ276 and NJ341 were 23.47 % and 32.15 %, respectively, after 20 days culturation at 5 ℃, and the rates were 43.95 % and 62.47 % respectively after 20 days culturation at 15 ℃. The oil degradation abilities were enhanced remarkably with the increasing culture temperature. GC - MSs indicated the residual oil contained C15 - C21 7 alkyls after degradation by NJ276, and C16, C17 and C18 3 alkyls after degradation by NJ341. The 16S rDNA gene sequences homology and phylogenetic analysis of the two Antarctic psychrophile bacteria showed that NJ276 belonged to the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia.展开更多
基金the Natural Science Foundation of China(No.40876107).
文摘Two hydrocarbon degrading bacteria NJ276 and NJ341 were screened from 385 Antarctic marine bacteria and their degrading characteristics were studied. Diesel oil as sole carbon source was used in this study. The results showed that the oil degradation rates of Antarctic psychrophile bacteria NJ276 and NJ341 were 23.47 % and 32.15 %, respectively, after 20 days culturation at 5 ℃, and the rates were 43.95 % and 62.47 % respectively after 20 days culturation at 15 ℃. The oil degradation abilities were enhanced remarkably with the increasing culture temperature. GC - MSs indicated the residual oil contained C15 - C21 7 alkyls after degradation by NJ276, and C16, C17 and C18 3 alkyls after degradation by NJ341. The 16S rDNA gene sequences homology and phylogenetic analysis of the two Antarctic psychrophile bacteria showed that NJ276 belonged to the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia.
