Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupunctur...Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.展开更多
The function of stomata in plants is controlling gas exchange and modulating water balance. The distribution pattern of stomata in most vascular plants follows a certain regulation with at least one normal epidermal c...The function of stomata in plants is controlling gas exchange and modulating water balance. The distribution pattern of stomata in most vascular plants follows a certain regulation with at least one normal epidermal cell between two stomata. However, some plants restricted in several genera of vascular plants have stomatal clusters in which more than one stoma is adjacently arranged with no epidermal cells among them. The developmental process of stomatal clusters in plants, especially in non-mutant (wild type) vascular plants, has rarely been documented, and very few studies concerning the distribution pattern of stomatal clusters on leaf epidermis have been carried out. We reported the developmental mechanism and distribution pattern of stomatal clusters in Begonia peltatifolia Li native to China. The results indicated that the clustered arrangement of meristemoids at the juvenile stage of the leaf development contributed greatly to the pattern of stomatal clusters. Additionally, satellite meristemoids derived from subsidiary cells around the mature stomata also had an impact on the development as well as the pattern of stomatal clusters. Regarding stomatal cluster and singly occurring stoma both as a stomatal unit, we found that the stomatal unit density (i.e., number of stomatal unit per area) increased gradually from the middle part to the edge and the apex of the leaf, while stomatal unit size (i.e., number of stomata per stomatal unit) decreased. The possible reason of this pattern was discussed.展开更多
[Objective] This study was to investigate the effects of fulvic acid on agronomic traits and yield of autumn potato. [Method] The effects of fulvic acid on growth period, plant morphology, tuber, net photosynthetic ra...[Objective] This study was to investigate the effects of fulvic acid on agronomic traits and yield of autumn potato. [Method] The effects of fulvic acid on growth period, plant morphology, tuber, net photosynthetic rate, chlorophyll content, leaf area index （LAI） and yield of autumn potato were investigated. [Result] The application of fulvic acid prolonged the growth period and increased the plant height, stem diameter, leaf chlorophyll content, LAI, net photosynthetic rate, tuber number per plant, tuber weight per plant and marketable tuber rate of autumn potato, thus increasing the yield. Applying fulvic acid prolonged the growth period of autumn potato by 1-3 d and significantly increased the plant height, net photosynthetic rate, tuber weight per plant and yield of autumn potato （P〈0.05, P〈0.01）. When the application amount of compound fertilizer was reduced by half, applying fulvic acid almost showed the same seedling emergence stage, mature stage, growth period and net photosynthetic rate with applying full amount of compound fertilizer. In particular, no significant difference was found in the yield of autumn potato between fullamount and half-amount compound fertilizer treatment groups in the premise of solid- liquid fulvic acid combined application. [Conclusion] In the premise of unchanged yield, applying fulvic acid can reduce the application of chemical fertilizers and reduce the non-point source pollution of chemical fertilizers.展开更多
文摘Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.
文摘The function of stomata in plants is controlling gas exchange and modulating water balance. The distribution pattern of stomata in most vascular plants follows a certain regulation with at least one normal epidermal cell between two stomata. However, some plants restricted in several genera of vascular plants have stomatal clusters in which more than one stoma is adjacently arranged with no epidermal cells among them. The developmental process of stomatal clusters in plants, especially in non-mutant (wild type) vascular plants, has rarely been documented, and very few studies concerning the distribution pattern of stomatal clusters on leaf epidermis have been carried out. We reported the developmental mechanism and distribution pattern of stomatal clusters in Begonia peltatifolia Li native to China. The results indicated that the clustered arrangement of meristemoids at the juvenile stage of the leaf development contributed greatly to the pattern of stomatal clusters. Additionally, satellite meristemoids derived from subsidiary cells around the mature stomata also had an impact on the development as well as the pattern of stomatal clusters. Regarding stomatal cluster and singly occurring stoma both as a stomatal unit, we found that the stomatal unit density (i.e., number of stomatal unit per area) increased gradually from the middle part to the edge and the apex of the leaf, while stomatal unit size (i.e., number of stomata per stomatal unit) decreased. The possible reason of this pattern was discussed.
基金Supported by Program for Potato Innovative Research Team in Sichuan ProvinceScience and Technology Support Program of Nanchong City~~
文摘[Objective] This study was to investigate the effects of fulvic acid on agronomic traits and yield of autumn potato. [Method] The effects of fulvic acid on growth period, plant morphology, tuber, net photosynthetic rate, chlorophyll content, leaf area index （LAI） and yield of autumn potato were investigated. [Result] The application of fulvic acid prolonged the growth period and increased the plant height, stem diameter, leaf chlorophyll content, LAI, net photosynthetic rate, tuber number per plant, tuber weight per plant and marketable tuber rate of autumn potato, thus increasing the yield. Applying fulvic acid prolonged the growth period of autumn potato by 1-3 d and significantly increased the plant height, net photosynthetic rate, tuber weight per plant and yield of autumn potato （P〈0.05, P〈0.01）. When the application amount of compound fertilizer was reduced by half, applying fulvic acid almost showed the same seedling emergence stage, mature stage, growth period and net photosynthetic rate with applying full amount of compound fertilizer. In particular, no significant difference was found in the yield of autumn potato between fullamount and half-amount compound fertilizer treatment groups in the premise of solid- liquid fulvic acid combined application. [Conclusion] In the premise of unchanged yield, applying fulvic acid can reduce the application of chemical fertilizers and reduce the non-point source pollution of chemical fertilizers.
