To clarify the antibacterial behavior at early adhesion,two titania nanotube(TNT)arrays were fabricated on polished commercially pure titanium(Ti),and the interaction mechanisms between TNT arrays and the model bacter...To clarify the antibacterial behavior at early adhesion,two titania nanotube(TNT)arrays were fabricated on polished commercially pure titanium(Ti),and the interaction mechanisms between TNT arrays and the model bacteria(Escherichia coli,E.coli)were investigated.The results show that TNT arrays exhibit a significant early antibacterial effect,which is highly related to the surface free energy and nano-topography.The underlying antibacterial mechanisms include:(1)the anti-initial-attachment effect at the lag phase(0−4 h);(2)the anti-proliferation and physical bactericidal effects at the logarithmic phase(4−12 h);(3)the reduced antimicrobial properties probably due to the overgrowth of bacteria on TNT arrays at the stationary phase(12 h and then).展开更多
AIM: Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aim...AIM: Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aimed at determining whether H pylori directly stimulates release of MIF in monocytes, whether the cag pathogenicity island (PAI) is involved for this function, and whether MIF stimulated by H pylori increases gastric epithelial cell proliferation in vitro. METHODS: A cytotoxic wild-type H pylori strain (TN2), its three isogenic mutants (TN2Δcag,TN2ΔcagA and TN2ΔcagE) were co-cultured with cells of a human monocyte cell line, THP-1, for 24 h at different organism/ cell ratios. MIF in the supernatants was measured by an ELISA. Cells of a human gastric cancer cell line, MKN45, were then co-cultured with the supernatants, with and without monoclonal anti-MIF antibody for 24 h. The cells were further incubated for 12 h after addition of 3H-thymidine, and the levels of incorporation of 3H-thymidine were measured with a liquid scintillation counter. RESULTS: The wild-type strain and the isogenic mutants, TN2ΔcagA and TN2ΔcagE, increased MIF release at organism/cell ratios of 200/1 and 400/1, but not at the ratios of 50/1 and 100/1. However, the mutant TN2Δcag did not increase the release of MIF at any of the four ratios. 3H-thymidine readings for MKN-45 cells were significantly increased with supernatants derived from the wild-type strain and the mutants TN2ΔcagA and TN2ΔcagE, but not from the mutant TN2Δcag. Moreover, in the presence of monoclonal anti-MIF antibody, the stimulatory effects of the wild-type strain on cell proliferation disappeared. CONCLUSION: H pylori stimulates MIF release in monocytes, likely through its cag PAI, but not related to cagA or cagE. H pylori-stimulated monocyte culture supernatant increases gastric cell proliferation, which is blocked by anti-MIF antibody, suggesting that MIF plays an important role in H pylori-induced gastric epithelial cell proliferation.展开更多
Objective:To study the inhibitory effect of docetaxel(DOC),oxaliplatin(OXA) and their combination on proliferation of cervical cancer line HeLa.Methods:Cell morphological changes were observed by inverted phase contra...Objective:To study the inhibitory effect of docetaxel(DOC),oxaliplatin(OXA) and their combination on proliferation of cervical cancer line HeLa.Methods:Cell morphological changes were observed by inverted phase contrast microscope,cell inhibition rates in different groups were examined by MTT,and cell cycle and apoptosis rates were determined by flow cytometry(FCM).Results:DOC and OXA inhibited the proliferation of cervical cancer cell line HeLa in a dose-dependent,and combination of the two drugs had an enhanced inhibitory effect;the apoptosis rate was also significantly increased when the two drugs were used in combination.Conclusion:DOC and OXA can synergistically inhibit the proliferation of cervical cancer cell line HeLa,which indicates that combination of the two drugs might has a promising future for clinical treatment of cervical cancer.展开更多
基金The authors are grateful for the financial supports from the National Natural Science Foundation of China(Nos.51604104,52171114,81702709)the Innovation-oriented Advanced Technology and Industrial Technology Program Project of Hunan Province,China(No.2020SK2017)+2 种基金the Central South University Postgraduate Education and Teaching Reform Project,China(No.2020JGB116)the Natural Science Foundation of Hunan Province,China(No.2020JJ4459)the Fundamental Research Funds for the Central Universities of Central South University,China(No.CX20200267).
文摘To clarify the antibacterial behavior at early adhesion,two titania nanotube(TNT)arrays were fabricated on polished commercially pure titanium(Ti),and the interaction mechanisms between TNT arrays and the model bacteria(Escherichia coli,E.coli)were investigated.The results show that TNT arrays exhibit a significant early antibacterial effect,which is highly related to the surface free energy and nano-topography.The underlying antibacterial mechanisms include:(1)the anti-initial-attachment effect at the lag phase(0−4 h);(2)the anti-proliferation and physical bactericidal effects at the logarithmic phase(4−12 h);(3)the reduced antimicrobial properties probably due to the overgrowth of bacteria on TNT arrays at the stationary phase(12 h and then).
基金Supported by a Competitive Earmarked Research Grant from the Research Grants Council of Hong Kong Special Administrative Region, China HKU7318/01M
文摘AIM: Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aimed at determining whether H pylori directly stimulates release of MIF in monocytes, whether the cag pathogenicity island (PAI) is involved for this function, and whether MIF stimulated by H pylori increases gastric epithelial cell proliferation in vitro. METHODS: A cytotoxic wild-type H pylori strain (TN2), its three isogenic mutants (TN2Δcag,TN2ΔcagA and TN2ΔcagE) were co-cultured with cells of a human monocyte cell line, THP-1, for 24 h at different organism/ cell ratios. MIF in the supernatants was measured by an ELISA. Cells of a human gastric cancer cell line, MKN45, were then co-cultured with the supernatants, with and without monoclonal anti-MIF antibody for 24 h. The cells were further incubated for 12 h after addition of 3H-thymidine, and the levels of incorporation of 3H-thymidine were measured with a liquid scintillation counter. RESULTS: The wild-type strain and the isogenic mutants, TN2ΔcagA and TN2ΔcagE, increased MIF release at organism/cell ratios of 200/1 and 400/1, but not at the ratios of 50/1 and 100/1. However, the mutant TN2Δcag did not increase the release of MIF at any of the four ratios. 3H-thymidine readings for MKN-45 cells were significantly increased with supernatants derived from the wild-type strain and the mutants TN2ΔcagA and TN2ΔcagE, but not from the mutant TN2Δcag. Moreover, in the presence of monoclonal anti-MIF antibody, the stimulatory effects of the wild-type strain on cell proliferation disappeared. CONCLUSION: H pylori stimulates MIF release in monocytes, likely through its cag PAI, but not related to cagA or cagE. H pylori-stimulated monocyte culture supernatant increases gastric cell proliferation, which is blocked by anti-MIF antibody, suggesting that MIF plays an important role in H pylori-induced gastric epithelial cell proliferation.
文摘Objective:To study the inhibitory effect of docetaxel(DOC),oxaliplatin(OXA) and their combination on proliferation of cervical cancer line HeLa.Methods:Cell morphological changes were observed by inverted phase contrast microscope,cell inhibition rates in different groups were examined by MTT,and cell cycle and apoptosis rates were determined by flow cytometry(FCM).Results:DOC and OXA inhibited the proliferation of cervical cancer cell line HeLa in a dose-dependent,and combination of the two drugs had an enhanced inhibitory effect;the apoptosis rate was also significantly increased when the two drugs were used in combination.Conclusion:DOC and OXA can synergistically inhibit the proliferation of cervical cancer cell line HeLa,which indicates that combination of the two drugs might has a promising future for clinical treatment of cervical cancer.
基金Supported by the Fundamental Research Funds for the Central Universities(GK201703068)by the University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization(1111090010)~~
基金Supported by the Fundamental Research Funds for the Central Universities(GK201703068)the University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization(1111090010)~~
