Interaction of a drug molecule with human serum albumin （HSA） is usually studied by fluorescence responses of the ligand or/and the single tryptophan residue （Trp-214） of the protein, but qualitative spectral info...Interaction of a drug molecule with human serum albumin （HSA） is usually studied by fluorescence responses of the ligand or/and the single tryptophan residue （Trp-214） of the protein, but qualitative spectral information may lead to multiple conclusions. In this work, we report a study on the interaction of hematoporphyrin monomethyl ether （HMME） with human serum albumin （HSA）, using the environment-sensitive spectra of HMME and reaction-induced fluorescence response of Trp-214. Particularly, the single kinetic parameter, the linear slope, was derived from the concentration-dependent absorbance or fluorescence of HMME in a certain solvent. A quantitative change in the slope at [HMME]/[HSA] = 1 ： 1 clearly demonstrated a specific binding of HMME to site I. The microenvironment in site I may be comparable to that in DMSO solvent, because of the similarity of the slope. Linear correlation of the fluorescence to the absorbance of HMME in site I indicates that the energy transfer is not responsible for Trp-214 fluorescence quenching but an electron transfer may be possible. In addition, much higher rate observed for the binding of HMME or 2-taurine-substituted HB （THB） with HSA than that of hypocrellin B was due to the electrostatic attraction under physiological condition.展开更多
In order to realize the online measurement of lamp dimension,the bulb image dimension measurement based on vision(BIDMV)is proposed.The image of lamp is obtained by camera.After image processing,such as Otsu algorithm...In order to realize the online measurement of lamp dimension,the bulb image dimension measurement based on vision(BIDMV)is proposed.The image of lamp is obtained by camera.After image processing,such as Otsu algorithm,median filter,ellipse fitting and envelope rectangle fitting,the dimension of lamp can be calculated.Based on this method,a non-contact real-time measurement system of the lamp’s dimension is developed.The precision of the proposed method is 0.07 mm,and it can satisfy the tolerance of the National Standard GB15766.1-2008.The experiment results show that the proposed method has a faster measuring speed and a higher precision compared with other measurement methods.展开更多
基金National Natural Science Foundation of China(Grant No.20872144)
文摘Interaction of a drug molecule with human serum albumin （HSA） is usually studied by fluorescence responses of the ligand or/and the single tryptophan residue （Trp-214） of the protein, but qualitative spectral information may lead to multiple conclusions. In this work, we report a study on the interaction of hematoporphyrin monomethyl ether （HMME） with human serum albumin （HSA）, using the environment-sensitive spectra of HMME and reaction-induced fluorescence response of Trp-214. Particularly, the single kinetic parameter, the linear slope, was derived from the concentration-dependent absorbance or fluorescence of HMME in a certain solvent. A quantitative change in the slope at [HMME]/[HSA] = 1 ： 1 clearly demonstrated a specific binding of HMME to site I. The microenvironment in site I may be comparable to that in DMSO solvent, because of the similarity of the slope. Linear correlation of the fluorescence to the absorbance of HMME in site I indicates that the energy transfer is not responsible for Trp-214 fluorescence quenching but an electron transfer may be possible. In addition, much higher rate observed for the binding of HMME or 2-taurine-substituted HB （THB） with HSA than that of hypocrellin B was due to the electrostatic attraction under physiological condition.
基金supported by the National Natural Science Foundation of China(Nos.60808020 and 61078041)the National Science and Technology Support(No.2014BAH03F01)+1 种基金the Tianjin Research Program of Application Foundation and Advanced Technology(No.10JCYBJC07200)the Technology Program of Tianjin Municipal Education Commission(No.20130324)
文摘In order to realize the online measurement of lamp dimension,the bulb image dimension measurement based on vision(BIDMV)is proposed.The image of lamp is obtained by camera.After image processing,such as Otsu algorithm,median filter,ellipse fitting and envelope rectangle fitting,the dimension of lamp can be calculated.Based on this method,a non-contact real-time measurement system of the lamp’s dimension is developed.The precision of the proposed method is 0.07 mm,and it can satisfy the tolerance of the National Standard GB15766.1-2008.The experiment results show that the proposed method has a faster measuring speed and a higher precision compared with other measurement methods.
