BCL2 is best known as a multifnnctional anti-apoptotic protein. However, little is known about its role in cell- adhesive and motility events. Here, we show that BCL2 may play a role in the regulation of cell adhesion...BCL2 is best known as a multifnnctional anti-apoptotic protein. However, little is known about its role in cell- adhesive and motility events. Here, we show that BCL2 may play a role in the regulation of cell adhesion, spreading, and motility. When BCL2 was overexpressed in cultured murine and human cell lines, cell spreading, adhesion, and motility were impaired. Consistent with these results, the loss of Bcl2 resulted in higher motility observed in Bcl2- null mouse embryonic fibroblast （MEF） cells compared to wild type. The mechanism of BCL2 regulation of cell adhesion and motility may involve formation of a complex containing BCL2, actin, and gelsolin, which appears to functionally decrease the severing activity of gelsolin. We have observed that the lysate from MCF-7 and NIH3T3 cells that overexpressed BCL2 enhanced actin polymerization in cell-free in vitro assays. Confocal immunofluorescent localization of BCL2 and F-actin during spreading consistently showed that increased expression of BCL2 resulted in increased F-actin polymerization. Thus, the formation of BCL2 and gelsolin complexes （which possibly contain other proteins） appears to play a critical role in the regulation of cell adhesion and migration. Given the established correlation of cell motility with cancer metastasis, this result may explain why the expression Of BCL2 in some tumor cell types reduces the potential for metastasis and is associated with improved patient prognosis.展开更多
The competition between attraction and diffusion determines the kinetics of non-equilibrium aggregation process.The formation of silver nanoclusters through non-equilibrium aggregation of silver atoms in solution was ...The competition between attraction and diffusion determines the kinetics of non-equilibrium aggregation process.The formation of silver nanoclusters through non-equilibrium aggregation of silver atoms in solution was simulated by molecular dynamics as a model system to study the influence of the competition between attraction and diffusion on the aggregation process by varying concentration and temperature.It has been found that the aggregation time decreases monotonically with increasing concentration of silver atoms because of increasing attraction,while initially decreasing and then increasing with increasing temperature because of the competition between accelerated attractive motion and increasing diffusive motion of silver atoms.A mean field approximation was employed to develop a phenomenological model describing the mechanism of temperature dependence of aggregation time.展开更多
文摘BCL2 is best known as a multifnnctional anti-apoptotic protein. However, little is known about its role in cell- adhesive and motility events. Here, we show that BCL2 may play a role in the regulation of cell adhesion, spreading, and motility. When BCL2 was overexpressed in cultured murine and human cell lines, cell spreading, adhesion, and motility were impaired. Consistent with these results, the loss of Bcl2 resulted in higher motility observed in Bcl2- null mouse embryonic fibroblast （MEF） cells compared to wild type. The mechanism of BCL2 regulation of cell adhesion and motility may involve formation of a complex containing BCL2, actin, and gelsolin, which appears to functionally decrease the severing activity of gelsolin. We have observed that the lysate from MCF-7 and NIH3T3 cells that overexpressed BCL2 enhanced actin polymerization in cell-free in vitro assays. Confocal immunofluorescent localization of BCL2 and F-actin during spreading consistently showed that increased expression of BCL2 resulted in increased F-actin polymerization. Thus, the formation of BCL2 and gelsolin complexes （which possibly contain other proteins） appears to play a critical role in the regulation of cell adhesion and migration. Given the established correlation of cell motility with cancer metastasis, this result may explain why the expression Of BCL2 in some tumor cell types reduces the potential for metastasis and is associated with improved patient prognosis.
基金supported by the National Natural Science Foundation of China(Grant Nos.10974208 and 11121403)the Program of "One Hundred Talented People" of the Chinese Academy of Sciences
文摘The competition between attraction and diffusion determines the kinetics of non-equilibrium aggregation process.The formation of silver nanoclusters through non-equilibrium aggregation of silver atoms in solution was simulated by molecular dynamics as a model system to study the influence of the competition between attraction and diffusion on the aggregation process by varying concentration and temperature.It has been found that the aggregation time decreases monotonically with increasing concentration of silver atoms because of increasing attraction,while initially decreasing and then increasing with increasing temperature because of the competition between accelerated attractive motion and increasing diffusive motion of silver atoms.A mean field approximation was employed to develop a phenomenological model describing the mechanism of temperature dependence of aggregation time.
