目的ω-芋螺毒素SO3是通过分子生物学手段从海洋生物线纹芋螺中提取的一种多肽,为新型、特异性N型电压敏感性钙离子通道阻滞剂。观察鞘内注射(it)ω-芋螺毒素SO3对大鼠坐骨神经慢性压迫性损伤(CC I模型)所致神经痛的镇痛作用,以及对背...目的ω-芋螺毒素SO3是通过分子生物学手段从海洋生物线纹芋螺中提取的一种多肽,为新型、特异性N型电压敏感性钙离子通道阻滞剂。观察鞘内注射(it)ω-芋螺毒素SO3对大鼠坐骨神经慢性压迫性损伤(CC I模型)所致神经痛的镇痛作用,以及对背根神经节(DRG)细胞内Ca2+含量的影响。方法♂SD大鼠40只,随机均分为5组,即正常对照组(N组)、CC I后14 d组(C组)、CC I 14 d it生理盐水组(CN组)、CC I 14 d it SO3 600 ng组(CS 1组)和CC I 7 d后连续鞘内注射SO3 30 ng.h-1共7 d组(CS 7组)。观察各组动物热痛觉过敏及机械刺激痛觉异常的反应阈值,并测定DRG细胞内Ca2+含量。结果CC I 14 d时结扎侧与非结扎侧热及机械刺激痛阈值均下降,同时DRG细胞内Ca2+含量也升高。it SO3后,结扎侧及非结扎侧痛阈值均升高,而DRG细胞内Ca2+含量降低。结论it SO3对慢性神经痛有镇痛作用,同时可以抑制CC I引起的DRG细胞内Ca2+含量增加,提示DRG细胞N型Ca2+电流在此伤害性信息传递中起作用。展开更多
Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus...Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus caracteristicus collected from South China Sea was studied in this work.A new conotoxin,cr5a,was isolated from the venom through Sephadex G-25 chromatography and HPLC,and then was identified by（amino） acid analysis,mass spectrometry,N-terminal sequence.The primary structure of cr5a was CCKFQFLNFCCNE,it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues（CC-CC）.Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved,and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys1-Cys10, Cys2-Cys11.So cr5a is a new member of the T-superfamily conotoxins.展开更多
文摘目的ω-芋螺毒素SO3是通过分子生物学手段从海洋生物线纹芋螺中提取的一种多肽,为新型、特异性N型电压敏感性钙离子通道阻滞剂。观察鞘内注射(it)ω-芋螺毒素SO3对大鼠坐骨神经慢性压迫性损伤(CC I模型)所致神经痛的镇痛作用,以及对背根神经节(DRG)细胞内Ca2+含量的影响。方法♂SD大鼠40只,随机均分为5组,即正常对照组(N组)、CC I后14 d组(C组)、CC I 14 d it生理盐水组(CN组)、CC I 14 d it SO3 600 ng组(CS 1组)和CC I 7 d后连续鞘内注射SO3 30 ng.h-1共7 d组(CS 7组)。观察各组动物热痛觉过敏及机械刺激痛觉异常的反应阈值,并测定DRG细胞内Ca2+含量。结果CC I 14 d时结扎侧与非结扎侧热及机械刺激痛阈值均下降,同时DRG细胞内Ca2+含量也升高。it SO3后,结扎侧及非结扎侧痛阈值均升高,而DRG细胞内Ca2+含量降低。结论it SO3对慢性神经痛有镇痛作用,同时可以抑制CC I引起的DRG细胞内Ca2+含量增加,提示DRG细胞N型Ca2+电流在此伤害性信息传递中起作用。
文摘Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus caracteristicus collected from South China Sea was studied in this work.A new conotoxin,cr5a,was isolated from the venom through Sephadex G-25 chromatography and HPLC,and then was identified by（amino） acid analysis,mass spectrometry,N-terminal sequence.The primary structure of cr5a was CCKFQFLNFCCNE,it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues（CC-CC）.Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved,and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys1-Cys10, Cys2-Cys11.So cr5a is a new member of the T-superfamily conotoxins.
