目的探讨慢性阻塞性肺疾病急性加重(acute exacerbation of chronic obstructive pulmonary disease,AECOPD)继发肺真菌感染患者外周血辅助型T细胞17(helper T cells 17,Th17)/调节型T细胞(regulatory T cells,Treg)比值及血清细胞因子...目的探讨慢性阻塞性肺疾病急性加重(acute exacerbation of chronic obstructive pulmonary disease,AECOPD)继发肺真菌感染患者外周血辅助型T细胞17(helper T cells 17,Th17)/调节型T细胞(regulatory T cells,Treg)比值及血清细胞因子的变化。方法选择南通大学附属海安人民医院呼吸内科2018年1月至2020年3月收治的AECOPD合并真菌感染27例患者(真菌感染组)的临床资料进行回顾性分析,采用病例对照研究的方法,收集同期接受治疗的AECOPD未合并真菌感染58例患者(非真菌感染组)临床资料,比较两组患者一般临床资料、外周血Th17、Treg水平、Th17/Treg比值及白细胞介素17(interleukin-17,IL-17)、白细胞介素23(interleukin-23,IL-23)、干扰素γ、转化生长因子β(transforming growth factor-β,TGF-β)水平,同时比较真菌感染组不同感染严重程度患者外周血Th17、Treg水平、Th17/Treg比值及血清细胞因子的变化。呈正态分布的计量资料两组间比较采用两独立样本t检验;多组间比较采用单因素方差分析,两两比较采用LSD-t检验。计数资料采用χ^(2)检验。结果27例AECOPD合并真菌感染组患者病原菌分布:白色假丝酵母菌占65.52%(19/27),热带假丝酵母菌占10.34%(3/27),近平滑假丝酵母菌占10.34%(3/27),曲霉菌占6.90%(2/27)。与非真菌感染组比较,真菌感染组患者Th17[(16.18±3.15)%与(12.34±2.64)%,t=5.87,P<0.001]、Th17/Treg比值[(4.70±0.85)与(2.41±0.51),t=22.87,P<0.001]、IL-17[(85.67±21.51)μg/L与(53.64±14.36)μg/L,t=8.12,P<0.001]、IL-23[(61.38±16.58)μg/L与(38.29±12.60)μg/L,t=7.10,P<0.001]均升高,Treg[(3.42±0.42)%与(5.13±0.51)%,t=20.77,P<0.001]、干扰素γ[(47.75±17.72)μg/L与(62.37±19.06)μg/L,t=3.37,P=0.001]、TGF-β[(110.34±26.03)μg/L与(131.40±35.03)μg/L,t=2.87,P=0.007]均降低,两组比较差异均有统计学意义。轻度、中度、重度真菌感染组患者Th17[(13.06±1.98)%、(15.94±2.11)%、(17.75±2.20)%,F=10.19,P<0.001]、Th17/Treg(5.01±0.60、5.66±0.69、6.52±0.65,F=10.77,P<0.001)、IL-17[(63.39±11.64)、(78.66±12.82)、(90.26±13.55)μg/L,F=9.01,P=0.001]、IL-23[(42.52±13.11)、(59.97±15.25)、(69.75±14.30)μg/L,F=7.41,P=0.003]随着感染严重程度的增加而升高;Treg[(4.33±0.39)%、(3.32±0.42)%、(2.50±0.35)%,F=44.42,P<0.001]、干扰素γ[(57.78±10.52)、(48.82±10.39)、(38.90±10.56)μg/L,F=6.50,P=0.006]、TGF-β[(126.62±18.94)、(115.34±13.66)、(102.52±17.73)μg/L,F=4.25,P=0.026]随着感染严重程度的增加而降低;差异均有统计学意义。结论Th17/Treg比值及相关炎症因子水平失衡可能对AECOPD继发真菌感染存在影响,且其失衡与真菌感染严重程度需密切监测患者外周血Th17、Treg水平及相关炎症因子的水平变化。展开更多
AIM: To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis (B. lactis) in an adoptive transfer model of colitis. METHODS: Donor and recipient mice received either B. lactis or bacterial cultur...AIM: To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis (B. lactis) in an adoptive transfer model of colitis. METHODS: Donor and recipient mice received either B. lactis or bacterial culture medium as control (deMan Rogosa Sharpe) in drinking water for one week prior to transfer of a mix of naive and regulatory T cells until sacrifice. RESULTS: All recipient mice developed signs of colonic inflammation, but a significant reduction of weight loss was observed in B. lactis-fed recipient mice compared to control mice. Moreover, a trend toward a diminution of mucosal thickness and attenuated epithelial damage was revealed. Colonic expression of pro-inflammatory and T cell markers was significantly reduced in B. lactis-fed recipient mice compared to controls. Concomitantly, forkhead box protein 3, a marker of regulatory T cells, was significantly up-regulated by B. lactis. CONCLUSION: Daily oral administration of B. lactis was able to reduce inflammatory and T cells mediators and to promote regulatory T cells specific markers in a mouse model of colitis.展开更多
目的探讨老年糖尿病患者口腔颌面部间隙感染(oral and maxillofacial space infection,OMSI)病原学特点及辅助性T细胞17(Th17)/调节性T细胞(Treg)细胞免疫的变化。方法选择三亚市人民医院老年科2017年1月-2019年3月收治的老年糖尿病患者...目的探讨老年糖尿病患者口腔颌面部间隙感染(oral and maxillofacial space infection,OMSI)病原学特点及辅助性T细胞17(Th17)/调节性T细胞(Treg)细胞免疫的变化。方法选择三亚市人民医院老年科2017年1月-2019年3月收治的老年糖尿病患者142例作为研究对象,根据患者是否发生OMSI分为感染组82例及非感染组60例。分析感染患者病原菌分布及耐药性,分别采用流式细胞术及酶联免疫吸附法检测两组患者外周血中Th17/Treg及白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、干扰素-γ(IFN-γ)和转化生长因子-β(TGF-β)水平。结果感染患者临床标本共检出病原菌87株,其中革兰阳性菌57株,占65.52%,革兰阴性菌30株,占34.48%;检出的主要革兰阳性菌对头孢曲松、头孢噻肟、头孢唑林和氨苄西林耐药率较高;检出的主要革兰阴性菌对左氧氟沙星、环丙沙星、庆大霉素耐药率较高;感染组Th17(17.52±2.18)%、Th17/Treg(3.01±0.24)、IL-17(65.67±18.24)pg/ml、IL-23(42.08±11.39)pg/ml均高于对照组(均P<0.05),Treg(5.83±1.06)%、IFN-γ(18.89±5.22)pg/ml、TGF-β水平(23.24±6.01)pg/ml均低于对照组(P<0.05)。结论老年糖尿病患者口腔颌面部间隙感染病原菌以金黄色葡萄球菌、表皮葡萄球菌、溶血链球菌、普雷沃氏菌和肺炎克雷伯菌检出率较高,可根据耐药性选用合理抗菌药物,感染患者Th17/Treg失衡,Th17细胞及促炎细胞因子表达升高,Treg细胞及抑炎细胞因子表达降低。展开更多
文摘目的探讨慢性阻塞性肺疾病急性加重(acute exacerbation of chronic obstructive pulmonary disease,AECOPD)继发肺真菌感染患者外周血辅助型T细胞17(helper T cells 17,Th17)/调节型T细胞(regulatory T cells,Treg)比值及血清细胞因子的变化。方法选择南通大学附属海安人民医院呼吸内科2018年1月至2020年3月收治的AECOPD合并真菌感染27例患者(真菌感染组)的临床资料进行回顾性分析,采用病例对照研究的方法,收集同期接受治疗的AECOPD未合并真菌感染58例患者(非真菌感染组)临床资料,比较两组患者一般临床资料、外周血Th17、Treg水平、Th17/Treg比值及白细胞介素17(interleukin-17,IL-17)、白细胞介素23(interleukin-23,IL-23)、干扰素γ、转化生长因子β(transforming growth factor-β,TGF-β)水平,同时比较真菌感染组不同感染严重程度患者外周血Th17、Treg水平、Th17/Treg比值及血清细胞因子的变化。呈正态分布的计量资料两组间比较采用两独立样本t检验;多组间比较采用单因素方差分析,两两比较采用LSD-t检验。计数资料采用χ^(2)检验。结果27例AECOPD合并真菌感染组患者病原菌分布:白色假丝酵母菌占65.52%(19/27),热带假丝酵母菌占10.34%(3/27),近平滑假丝酵母菌占10.34%(3/27),曲霉菌占6.90%(2/27)。与非真菌感染组比较,真菌感染组患者Th17[(16.18±3.15)%与(12.34±2.64)%,t=5.87,P<0.001]、Th17/Treg比值[(4.70±0.85)与(2.41±0.51),t=22.87,P<0.001]、IL-17[(85.67±21.51)μg/L与(53.64±14.36)μg/L,t=8.12,P<0.001]、IL-23[(61.38±16.58)μg/L与(38.29±12.60)μg/L,t=7.10,P<0.001]均升高,Treg[(3.42±0.42)%与(5.13±0.51)%,t=20.77,P<0.001]、干扰素γ[(47.75±17.72)μg/L与(62.37±19.06)μg/L,t=3.37,P=0.001]、TGF-β[(110.34±26.03)μg/L与(131.40±35.03)μg/L,t=2.87,P=0.007]均降低,两组比较差异均有统计学意义。轻度、中度、重度真菌感染组患者Th17[(13.06±1.98)%、(15.94±2.11)%、(17.75±2.20)%,F=10.19,P<0.001]、Th17/Treg(5.01±0.60、5.66±0.69、6.52±0.65,F=10.77,P<0.001)、IL-17[(63.39±11.64)、(78.66±12.82)、(90.26±13.55)μg/L,F=9.01,P=0.001]、IL-23[(42.52±13.11)、(59.97±15.25)、(69.75±14.30)μg/L,F=7.41,P=0.003]随着感染严重程度的增加而升高;Treg[(4.33±0.39)%、(3.32±0.42)%、(2.50±0.35)%,F=44.42,P<0.001]、干扰素γ[(57.78±10.52)、(48.82±10.39)、(38.90±10.56)μg/L,F=6.50,P=0.006]、TGF-β[(126.62±18.94)、(115.34±13.66)、(102.52±17.73)μg/L,F=4.25,P=0.026]随着感染严重程度的增加而降低;差异均有统计学意义。结论Th17/Treg比值及相关炎症因子水平失衡可能对AECOPD继发真菌感染存在影响,且其失衡与真菌感染严重程度需密切监测患者外周血Th17、Treg水平及相关炎症因子的水平变化。
文摘AIM: To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis (B. lactis) in an adoptive transfer model of colitis. METHODS: Donor and recipient mice received either B. lactis or bacterial culture medium as control (deMan Rogosa Sharpe) in drinking water for one week prior to transfer of a mix of naive and regulatory T cells until sacrifice. RESULTS: All recipient mice developed signs of colonic inflammation, but a significant reduction of weight loss was observed in B. lactis-fed recipient mice compared to control mice. Moreover, a trend toward a diminution of mucosal thickness and attenuated epithelial damage was revealed. Colonic expression of pro-inflammatory and T cell markers was significantly reduced in B. lactis-fed recipient mice compared to controls. Concomitantly, forkhead box protein 3, a marker of regulatory T cells, was significantly up-regulated by B. lactis. CONCLUSION: Daily oral administration of B. lactis was able to reduce inflammatory and T cells mediators and to promote regulatory T cells specific markers in a mouse model of colitis.
文摘目的探讨老年糖尿病患者口腔颌面部间隙感染(oral and maxillofacial space infection,OMSI)病原学特点及辅助性T细胞17(Th17)/调节性T细胞(Treg)细胞免疫的变化。方法选择三亚市人民医院老年科2017年1月-2019年3月收治的老年糖尿病患者142例作为研究对象,根据患者是否发生OMSI分为感染组82例及非感染组60例。分析感染患者病原菌分布及耐药性,分别采用流式细胞术及酶联免疫吸附法检测两组患者外周血中Th17/Treg及白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、干扰素-γ(IFN-γ)和转化生长因子-β(TGF-β)水平。结果感染患者临床标本共检出病原菌87株,其中革兰阳性菌57株,占65.52%,革兰阴性菌30株,占34.48%;检出的主要革兰阳性菌对头孢曲松、头孢噻肟、头孢唑林和氨苄西林耐药率较高;检出的主要革兰阴性菌对左氧氟沙星、环丙沙星、庆大霉素耐药率较高;感染组Th17(17.52±2.18)%、Th17/Treg(3.01±0.24)、IL-17(65.67±18.24)pg/ml、IL-23(42.08±11.39)pg/ml均高于对照组(均P<0.05),Treg(5.83±1.06)%、IFN-γ(18.89±5.22)pg/ml、TGF-β水平(23.24±6.01)pg/ml均低于对照组(P<0.05)。结论老年糖尿病患者口腔颌面部间隙感染病原菌以金黄色葡萄球菌、表皮葡萄球菌、溶血链球菌、普雷沃氏菌和肺炎克雷伯菌检出率较高,可根据耐药性选用合理抗菌药物,感染患者Th17/Treg失衡,Th17细胞及促炎细胞因子表达升高,Treg细胞及抑炎细胞因子表达降低。
