Despite that advances in neonatal medicine have significantly reduced the earl y mortality of premature infants, a considerable number of them are still prone to develop chronic lung disease (CLD) later. To find a met...Despite that advances in neonatal medicine have significantly reduced the earl y mortality of premature infants, a considerable number of them are still prone to develop chronic lung disease (CLD) later. To find a method of early preventio n, we investigated the efficacy of using certain early proinflammatory responses to predict the development of CLD. In the present study, 34 premature infants w ho required endotracheal intubation within 4 h of birth were recruited for analy sis of IL-8, IL-10, and TNF-αlevels in their bronchoalveolar lavage (BAL) fl uid and blood. It was found that level of IL-8 but not TNF-αor IL-10 in init ial BAL fluid was significantly correlated to neutrophils in the BAL and inverse ly correlated to the gestational age of prematurity. Elevation of IL-8 level in BAL on the first day of life was correlated to the development of CLD. Further studies showed that neonatal cord blood released significantly higher IL-8 but lower TNF-αlevels after stimulation by endotoxin. The augmented IL-8 mRNA exp ression in cord blood was inhibited by actinomycin D but enhanced by cycloheximi de, suggesting that IL-8 production is controlled by de novo transcriptional in duction as well as posttranscriptional up-regulation of IL-8 by neonatal leuko cytes, relating to the development of CLD. Thus, an appropriate modulation of in itial IL-8 production in premature infants might be beneficial for the preventi on of the development of CLD.展开更多
酸敏感离子通道（acid-sensing ion channels．ASICs）是一类广泛存在于细胞膜上的通透阳离子的蛋白复合体，属于上皮通道蜕变蛋白离子通道超家族，在感受体液pH值和调控痛觉、酸味觉等多项生理机能方面有着重要作用。炎症可诱导ASICs...酸敏感离子通道（acid-sensing ion channels．ASICs）是一类广泛存在于细胞膜上的通透阳离子的蛋白复合体，属于上皮通道蜕变蛋白离子通道超家族，在感受体液pH值和调控痛觉、酸味觉等多项生理机能方面有着重要作用。炎症可诱导ASICs转录并产生转录后调节，从而影响神经元兴奋性，参与痛觉感受的敏感化过程。其中3型ASIC3主要位于外周伤害性感受器，因能快速激活并介导稳态电流而被认为与痛觉过敏密切相关。本文就此离子通道的研究进展，与炎性痛敏的关系及其临床意义做一综述。展开更多
文摘Despite that advances in neonatal medicine have significantly reduced the earl y mortality of premature infants, a considerable number of them are still prone to develop chronic lung disease (CLD) later. To find a method of early preventio n, we investigated the efficacy of using certain early proinflammatory responses to predict the development of CLD. In the present study, 34 premature infants w ho required endotracheal intubation within 4 h of birth were recruited for analy sis of IL-8, IL-10, and TNF-αlevels in their bronchoalveolar lavage (BAL) fl uid and blood. It was found that level of IL-8 but not TNF-αor IL-10 in init ial BAL fluid was significantly correlated to neutrophils in the BAL and inverse ly correlated to the gestational age of prematurity. Elevation of IL-8 level in BAL on the first day of life was correlated to the development of CLD. Further studies showed that neonatal cord blood released significantly higher IL-8 but lower TNF-αlevels after stimulation by endotoxin. The augmented IL-8 mRNA exp ression in cord blood was inhibited by actinomycin D but enhanced by cycloheximi de, suggesting that IL-8 production is controlled by de novo transcriptional in duction as well as posttranscriptional up-regulation of IL-8 by neonatal leuko cytes, relating to the development of CLD. Thus, an appropriate modulation of in itial IL-8 production in premature infants might be beneficial for the preventi on of the development of CLD.
文摘酸敏感离子通道（acid-sensing ion channels．ASICs）是一类广泛存在于细胞膜上的通透阳离子的蛋白复合体，属于上皮通道蜕变蛋白离子通道超家族，在感受体液pH值和调控痛觉、酸味觉等多项生理机能方面有着重要作用。炎症可诱导ASICs转录并产生转录后调节，从而影响神经元兴奋性，参与痛觉感受的敏感化过程。其中3型ASIC3主要位于外周伤害性感受器，因能快速激活并介导稳态电流而被认为与痛觉过敏密切相关。本文就此离子通道的研究进展，与炎性痛敏的关系及其临床意义做一综述。
