This study aimed to understand the growing development and physiological characteristics of seeds harvested different days after pollination and the seedlings emerged from the seeds of yellow-seeded Brassica napus L.....This study aimed to understand the growing development and physiological characteristics of seeds harvested different days after pollination and the seedlings emerged from the seeds of yellow-seeded Brassica napus L..Two yellow-seeded cultivars‘Hongyuan 558’and‘Qinyou 806’were selected in this study,and 50 seeds were collected 30,35,and 40 d after pollination,respectively.The seeds were weighed and placed on filter paper for hydroponic experiments.The seed germination rate was determined 3-5 d later.The physiological indicators including superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)activities and malondialdehyde(MDA)content were measured for the seeds harvested different days after pollination and the 7-day-seedlings emerged from the seeds.The results showed that the seed dry weights and germination rates of both‘Qinyou 806’and‘Hongyuan 558’increased as the days after pollination increased,especially 30-35 d after pollination.The germination rate and dry and fresh weights of the seeds showed no significant differences between the two cultivars,and almost all the seeds germinated 40 d after pollination.As the days after pollination increased,the SOD activities in the seeds of both cultivars first increased and then decreased,reaching their peaks 35 d after pollination,which may be related to encountering adverse stress.The POD activity in the seeds of‘Qinyou 806’kept declining,and that of‘Hongyuan 558’first increased and then decreased,which may be related to the antioxidant mechanism and adaptive regulation.The CAT activities in the seeds of both cultivars first decreased and then increased.The MDA content in the seeds of‘Qinyou 806’first decreased and then increased,while that of‘Hongyuan 558’kept reducing and was the highest in the seeds 30 d after pollination,which indicated‘Hongyuan 558’was mildly affected by the environment and had strong resistance.The activities of SOD and POD in the seedlings of two cultivars that were emerged from the seeds 35-40 d after pollination became weakened,which may be related to their exposure to stress.The CAT activity in the seedlings of‘Hongyuan 558’was generally higher than that of‘Qinyou 806’,indicating that‘Hongyuan 558’had stronger stress resistance.The MDA content in the seedlings emerged from the seeds 35-40 d after pollination showed a decreasing trend,indicating that the seedlings emerged from the seeds harvested at this stage had mild cell membrane damage and enhanced stress resistance.In conclusion,‘Hongyuan 558’had strong environmental adaptability and stress resistance.展开更多
[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and H...[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and Huaimai 22, were selected as experimental materials and cultured under drought stress simulated by different concentrations of PEG6000, to determine the activity of POD and CAT. [Result] POD and CAT activity in wheat during generation period increased gradual y under stress of 5% and 15% PEG6000 from 0 to 120 h; POD and CAT activity in wheat under stress of 15% PEG6000 increased more compared with that under stress of 5% PEG6000; POD and CAT activity in wheat under stress of 25% and 35% PEG6000 increased first and then decreased. Stress duration significantly af-fected the activity of POD and CAT in wheat during generation period. [Conclusion] This study provided theoretical references for breeding drought-tolerant wheat.展开更多
[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua ...[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua No.11 as materials,the changes of rice seedlings under the treatment conditions of Cd,Cd＋CAT inhibitor,Cd＋APX inhibitor were studied.[Result] Under Cd stress,inhibition of CAT activity caused the significant inhibition on the growth of aerial parts,decreased the number of adventitious roots and lateral roots,but it can significant promote the elongation growth of adventitious roots and lateral roots.Moreover,the length of the first lateral root from root tip on the primary roots and adventitious roots was also increased than control.When APX activity was inhibited,the growth changes of rice were similar with that treated by CAT inhibitor.[Conclusion] CAT and APX may play important roles in the regulation of rice root system growth in both non-stress and Cd-stressed rice展开更多
[Objective] The effects of yttrium nitrate （YNO3） on biomass and antioxi- dant systems of paddy rice （Yttrium （Y）; Oxidative stress; Dismutases （SOD）; Per- oxidases （POD）, Catalases （CAT）, Paddy rice （Trit...[Objective] The effects of yttrium nitrate （YNO3） on biomass and antioxi- dant systems of paddy rice （Yttrium （Y）; Oxidative stress; Dismutases （SOD）; Per- oxidases （POD）, Catalases （CAT）, Paddy rice （Triticum aestivum）） together with the occurrences of Y in soils were investigated to assess its ecotoxicological effects on plant. [Method]Y solutions with various concentrations were sprinkled on soil sam- ples, which were well mixed and then put into culture dishes to culture paddy rice seeds for further evaluation. [Result] The results indicated that 25-100 mg/kg Y treatments significantly increased the biomass （total weight, root weight, shoot weight and leaf weight）, chlorophyll （CHL） content and protein content of paddy rice, whereas 200-800 mg/kg Y treatments had a converse effect. Similarly, biomarker for the antioxidant systems including superoxide dismutases （SOD）, peroxidases （POD） and catalases （CAT） all exhibited similar trends in both shoots and roots of paddy rice. At the same time, the malonaldehyde （MDA） content increased at from 25 to 100 mg/kg and decreased with concentrations of Y from 100 to 800 mg/kg in both shoots and roots of paddy rice. This indicated that Y could stimulate the growth of plant at low concentration, but inhibit the growth at relatively high concen- tration. [Conclusion] The levels of Y were 641＋49, 328_＋16 and 473_＋40 mg/kg in soils collected from mining area, farmland and navel orange orchard respectively. The levels of Y in the investigated area were higher than the benefit level （100 mg/kg）, which could cause low biomass as well as low activity of SOD, POD and CAT in paddy rice. Therefore, a more careful use of Y is necessary in crop management.展开更多
基金Supported by Major Project of Agricultural Biological Breeding(2023ZD0404205)Science and Technology Plan Project of Changsha City(kq2107016)。
文摘This study aimed to understand the growing development and physiological characteristics of seeds harvested different days after pollination and the seedlings emerged from the seeds of yellow-seeded Brassica napus L..Two yellow-seeded cultivars‘Hongyuan 558’and‘Qinyou 806’were selected in this study,and 50 seeds were collected 30,35,and 40 d after pollination,respectively.The seeds were weighed and placed on filter paper for hydroponic experiments.The seed germination rate was determined 3-5 d later.The physiological indicators including superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)activities and malondialdehyde(MDA)content were measured for the seeds harvested different days after pollination and the 7-day-seedlings emerged from the seeds.The results showed that the seed dry weights and germination rates of both‘Qinyou 806’and‘Hongyuan 558’increased as the days after pollination increased,especially 30-35 d after pollination.The germination rate and dry and fresh weights of the seeds showed no significant differences between the two cultivars,and almost all the seeds germinated 40 d after pollination.As the days after pollination increased,the SOD activities in the seeds of both cultivars first increased and then decreased,reaching their peaks 35 d after pollination,which may be related to encountering adverse stress.The POD activity in the seeds of‘Qinyou 806’kept declining,and that of‘Hongyuan 558’first increased and then decreased,which may be related to the antioxidant mechanism and adaptive regulation.The CAT activities in the seeds of both cultivars first decreased and then increased.The MDA content in the seeds of‘Qinyou 806’first decreased and then increased,while that of‘Hongyuan 558’kept reducing and was the highest in the seeds 30 d after pollination,which indicated‘Hongyuan 558’was mildly affected by the environment and had strong resistance.The activities of SOD and POD in the seedlings of two cultivars that were emerged from the seeds 35-40 d after pollination became weakened,which may be related to their exposure to stress.The CAT activity in the seedlings of‘Hongyuan 558’was generally higher than that of‘Qinyou 806’,indicating that‘Hongyuan 558’had stronger stress resistance.The MDA content in the seedlings emerged from the seeds 35-40 d after pollination showed a decreasing trend,indicating that the seedlings emerged from the seeds harvested at this stage had mild cell membrane damage and enhanced stress resistance.In conclusion,‘Hongyuan 558’had strong environmental adaptability and stress resistance.
基金Supported by Natural Science Fund of Education Department of Anhui Province(KJ2012B188)General Science and Research Program of Suzhou College(2011yyb13)~~
文摘[Objective] This study aimed to investigate the changes in activity of POD and CAT in wheat during generation period under drought stress. [Method] Four wheat cultivars, including Yunong 51, Zhoumai 22, Jimai 19 and Huaimai 22, were selected as experimental materials and cultured under drought stress simulated by different concentrations of PEG6000, to determine the activity of POD and CAT. [Result] POD and CAT activity in wheat during generation period increased gradual y under stress of 5% and 15% PEG6000 from 0 to 120 h; POD and CAT activity in wheat under stress of 15% PEG6000 increased more compared with that under stress of 5% PEG6000; POD and CAT activity in wheat under stress of 25% and 35% PEG6000 increased first and then decreased. Stress duration significantly af-fected the activity of POD and CAT in wheat during generation period. [Conclusion] This study provided theoretical references for breeding drought-tolerant wheat.
基金Supported by National Natural Science Foundation of China(30671126)~~
文摘[Objective] The research aimed to study the correlations between catalase（CAT） and ascorbate peroxidase（ASP） and the growth and development of rice roots under cadmium stress.[Method] Taking rice variety Zhonghua No.11 as materials,the changes of rice seedlings under the treatment conditions of Cd,Cd＋CAT inhibitor,Cd＋APX inhibitor were studied.[Result] Under Cd stress,inhibition of CAT activity caused the significant inhibition on the growth of aerial parts,decreased the number of adventitious roots and lateral roots,but it can significant promote the elongation growth of adventitious roots and lateral roots.Moreover,the length of the first lateral root from root tip on the primary roots and adventitious roots was also increased than control.When APX activity was inhibited,the growth changes of rice were similar with that treated by CAT inhibitor.[Conclusion] CAT and APX may play important roles in the regulation of rice root system growth in both non-stress and Cd-stressed rice
基金Supported by the National Natural Science Foundation of China(21067003,51364015)the National High-Tech Research and Development Program of China(2012BAC11B07)the Jiangxi Natural Science Foundation(20114BAB203024)~~
文摘[Objective] The effects of yttrium nitrate （YNO3） on biomass and antioxi- dant systems of paddy rice （Yttrium （Y）; Oxidative stress; Dismutases （SOD）; Per- oxidases （POD）, Catalases （CAT）, Paddy rice （Triticum aestivum）） together with the occurrences of Y in soils were investigated to assess its ecotoxicological effects on plant. [Method]Y solutions with various concentrations were sprinkled on soil sam- ples, which were well mixed and then put into culture dishes to culture paddy rice seeds for further evaluation. [Result] The results indicated that 25-100 mg/kg Y treatments significantly increased the biomass （total weight, root weight, shoot weight and leaf weight）, chlorophyll （CHL） content and protein content of paddy rice, whereas 200-800 mg/kg Y treatments had a converse effect. Similarly, biomarker for the antioxidant systems including superoxide dismutases （SOD）, peroxidases （POD） and catalases （CAT） all exhibited similar trends in both shoots and roots of paddy rice. At the same time, the malonaldehyde （MDA） content increased at from 25 to 100 mg/kg and decreased with concentrations of Y from 100 to 800 mg/kg in both shoots and roots of paddy rice. This indicated that Y could stimulate the growth of plant at low concentration, but inhibit the growth at relatively high concen- tration. [Conclusion] The levels of Y were 641＋49, 328_＋16 and 473_＋40 mg/kg in soils collected from mining area, farmland and navel orange orchard respectively. The levels of Y in the investigated area were higher than the benefit level （100 mg/kg）, which could cause low biomass as well as low activity of SOD, POD and CAT in paddy rice. Therefore, a more careful use of Y is necessary in crop management.
