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颤藻浓度和水温对凡纳滨对虾响应颤藻粗提液毒性的影响 被引量:7
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作者 徐煜 徐武杰 +5 位作者 文国樑 胡晓娟 苏浩昌 杨铿 李卓佳 曹煜成 《南方水产科学》 CAS CSCD 北大核心 2017年第1期26-32,共7页
文章研究了绿色颤藻(Oscillatoria chlorina)不同成分对凡纳滨对虾(Litopenaeus vannamei)的毒性,分析了颤藻浓度与水温对对虾响应粗提液毒性的影响,并初步检测了颤藻粗提液所含的毒素。结果显示,湿藻质量浓度为5.04 mg·m L^(-1)... 文章研究了绿色颤藻(Oscillatoria chlorina)不同成分对凡纳滨对虾(Litopenaeus vannamei)的毒性,分析了颤藻浓度与水温对对虾响应粗提液毒性的影响,并初步检测了颤藻粗提液所含的毒素。结果显示,湿藻质量浓度为5.04 mg·m L^(-1)的颤藻细胞及其粗提液可导致93.72%以上的对虾死亡,藻细胞培养液对对虾无致死效应。用湿藻质量浓度高于2.52 mg·m L^(-1)的粗提液注射对虾,对虾死亡率超过86.67%,随质量浓度低至0.504 mg·m L^(-1),对虾死亡率降至2.22%,差异显著(P<0.05);水温显著影响了粗提液对对虾的致死效应(P<0.05),湿藻质量浓度为2.52 mg·m L^(-1)的粗提液在22℃水温条件下可引起20.0%对虾死亡,当温度升至34℃,对虾死亡率增加至97.8%;以微囊藻毒素(Microcystin-RR/YR/LR,MC-RR/YR/LR)作标准品,使用高效液相色谱法检测颤藻粗提液毒素,结果在粗提液中均未检出RR、YR、LR等3种常见的微囊藻毒素。结果表明,绿色颤藻可致对虾急性死亡,其主要有害成分是颤藻粗提液所含的非MC-RR/YR/LR等藻毒素,对虾对颤藻粗提液毒性的响应与颤藻浓度和水温显著相关(P<0.05)。 展开更多
关键词 绿色 粗提液 颤藻浓度 水温 凡纳滨对虾
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The Inhibition Effects of Alkoxyl Thiourea Derivatives toward the Growth of Isolated Oscillatoria sp. from Kenyir Lake, Terengganu
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作者 Nor Azman Kasan Siti Zafirah Mohamad Yusof +2 位作者 Naqiyyu Bazz Ramli Wan M. Khairul Hazlina Ahamad Zakeri 《Journal of Environmental Science and Engineering(A)》 2015年第8期389-394,共6页
In controlling eutrophication phenomenon, there were conventional methods which lead to negative effects to aquatic environment. This study was aimed to investigate the usage of synthesized thiourea compounds to inhib... In controlling eutrophication phenomenon, there were conventional methods which lead to negative effects to aquatic environment. This study was aimed to investigate the usage of synthesized thiourea compounds to inhibit the growth of Oscillatoria sp. in Kenyir lake, Terengganu, Malaysia. The inhibition effects of four eco-friendly alkoxyl thiourea derivatives compounds named as N-((4-(decyloxy)phenyl)carbamothioyl)-4-methyl benzamide, N-((4-(decyloxy)phenyl)carbamothioyl)-4-nitro benzamide, 4-chloro-N-((4-(decyloxy)phenyl)carbamothioyl) benzamide and N-((4-(decyloxy)phenyl)carbamothioyl) benzamide were examined onto the growth culture of Oscillatoria sp. These compounds were tested in 30 mL of Oscillatoria sp. cultures with different concentration of 16 μg·mL-1, 18 μg·mL-1, 20 μg·mL-1, 24 μg·mL-1 and 28 μg·mL-1respectively. The treatment flasks were supplied with an aerator for 24 hours under continuous illumination at 25 ℃. Chlorophyll-a concentration were extracted to calculate the inhibition percentage of each treatment. Overall, all these compounds showed inhibition effects towards the growth of Oscillatoria sp., with the highest inhibition of 37% by N-((4-(decyloxy)phenyl)carbamothioyl)-4-methyl benzamide at the concentration of 18μg·mL-1. The methyl group that attach to the synthesized compound may contribute to the effectiveness of the compound which act as an algae inhibitor. However, extensive studies still need to be conducted in order to investigate the mechanism on how this compound reacts with Oscillatoria sp.. 展开更多
关键词 EUTROPHICATION alkoxyl thiourea Oscillatoria sp. CHLOROPHYLL-A inhibition effect.
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