Hypobaric hypoxia is the main environmental feature of the Tibetan plateau which would influence the efficiency of human metabolic heat production and the ability of thermal regulation.In order to understand the influ...Hypobaric hypoxia is the main environmental feature of the Tibetan plateau which would influence the efficiency of human metabolic heat production and the ability of thermal regulation.In order to understand the influence of the hypoxic environment on the plateau on the thermal comfort of short-term sojourners in Tibet,China,oxygen generators were used to create oxygen-enriched environments,and physiological and psychological reactions of subjects were compared under different oxygen partial pressures(p_(O_(2)))and air temperatures(t_(a)).The results showed that subjects’thermal sensation,thermal comfort and mean skin temperature decreased with a decrease in the oxygen partial pressure.When t_(a)=17℃,the influence of oxygen partial pressure was more pronounced,compared to p_(O_(2))=16.4 kPa,the thermal sensation of subjects under p_(O_(2))=13.7 kPa decreased by 33%.The rate of subjects feeling comfortable decreased by 25%,and the mean skin temperature decreased by 0.7℃.The hypoxic environment of the plateau exacerbates human discomfort.Therefore,it is necessary to fully understand the actual thermal requirements of sojourners in Tibet,China.The results of this study would have implications for a better understanding of thermal comfort characteristics in the hypoxia environment in plateau.展开更多
文摘有关动物适应异质性海拔环境的研究,已在表型、解剖学、生理学、基因组学和转录组学等方面广泛开展,但是很少关注小RNA(micro RNAs或miRNAs)的生态适应性功能.草原毛虫是青藏高原高寒草地的重要害虫,并对不同海拔环境产生了良好的适应性.为此,本研究以两种栖息于青藏高原不同海拔环境的黄斑草原毛虫和门源草原毛虫为研究对象,基于Illumina Hi Seq技术对这两种草原毛虫的miRNA转录组进行了高通量测序.结果表明,两种草原毛虫中共鉴定到109个成熟miRNAs,其中47个为保守miRNAs.在所有miRNAs种,共有9个miRNAs的表达水平在两种草原毛虫间存在显著差异性.对这9个差异性表达的miRNAs采用实时荧光定量PCR进行验证,发现8个基因的表达水平与高通量测序结果完全一致.功能分析表明,差异性表达的miRNAs共预测到93个靶基因.这些靶基因主要富集到脂、核酸和维生素等代谢或衰老相关的GO条目,并与代谢、抗寒和低氧等KEGG通路相关(如AMPK信号通路、Wnt信号通路和m TOR信号通路).这些研究表明,miRNAs在草原毛虫对异质性海拔环境的适应性进化过程中具有重要作用.该研究加深了对青藏高原特有昆虫适应高海拔环境的遗传基础的理解.
基金Project(U20A20311)supported by the State Key Program of National Natural Science Foundation of ChinaProject(52008329)supported by the National Natural Science Foundation of ChinaProject(2018BSHYDZZ14)supported by the Postdoctoral Research Foundation of Shaanxi Province,China。
文摘Hypobaric hypoxia is the main environmental feature of the Tibetan plateau which would influence the efficiency of human metabolic heat production and the ability of thermal regulation.In order to understand the influence of the hypoxic environment on the plateau on the thermal comfort of short-term sojourners in Tibet,China,oxygen generators were used to create oxygen-enriched environments,and physiological and psychological reactions of subjects were compared under different oxygen partial pressures(p_(O_(2)))and air temperatures(t_(a)).The results showed that subjects’thermal sensation,thermal comfort and mean skin temperature decreased with a decrease in the oxygen partial pressure.When t_(a)=17℃,the influence of oxygen partial pressure was more pronounced,compared to p_(O_(2))=16.4 kPa,the thermal sensation of subjects under p_(O_(2))=13.7 kPa decreased by 33%.The rate of subjects feeling comfortable decreased by 25%,and the mean skin temperature decreased by 0.7℃.The hypoxic environment of the plateau exacerbates human discomfort.Therefore,it is necessary to fully understand the actual thermal requirements of sojourners in Tibet,China.The results of this study would have implications for a better understanding of thermal comfort characteristics in the hypoxia environment in plateau.
