Cellular spheroids serving as three-dimensional(3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular...Cellular spheroids serving as three-dimensional(3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cellembedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.展开更多
A high-throughput multi-plume pulsed-laser deposition （MPPLD） system has been demonstrated and compared to previous techniques. Whereas most combinatorial pulsedlaser deposition （PLD） systems have focused on achie...A high-throughput multi-plume pulsed-laser deposition （MPPLD） system has been demonstrated and compared to previous techniques. Whereas most combinatorial pulsedlaser deposition （PLD） systems have focused on achieving thickness uniformity using sequential multilayer deposition and masking followed by post-deposition annealing, MPPLD directly deposits a compositionally varied library of compounds using the directionality of PLD plumes and the resulting spatial variations of deposition rate. This system is more suitable for high-throughput compound thin-film fabrication.展开更多
We present a scheme for probabilistic remote preparation of a tripartite qutrit entangled state with a partial tripartite qutrit entangled state and a partial bipartite qutrit entangled state as the quantum channel. I...We present a scheme for probabilistic remote preparation of a tripartite qutrit entangled state with a partial tripartite qutrit entangled state and a partial bipartite qutrit entangled state as the quantum channel. It is found that a bipartite qutrit orthogonal projective measurement, an auxiliary qutrit particle, and the corresponding unitary transformation are required. A scheme for probabilistic remote preparation of a tripartite qudit equatorial entangled state by using a partial tripartite qudit entangled state and a partial bipartite qudit entangled state as the quantum channel is also proposed. We calculate the successful total probability and the total classical communication cost required in the RSP process, respectively.展开更多
基金supported by the National Natural Science Foundation of China (11372243, 11532009, and 11522219)the China Postdoctoral Science Foundation (2013M540742)+2 种基金the Doctoral Program of Higher Education of China (20130201120071)the Natural Science Basic Research Plan in Shaanxi Province of China (2014JQ1004)the Fun- damental Research Funds for the Central Universities
文摘Cellular spheroids serving as three-dimensional(3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cellembedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.
基金partially supported by the US Department of Energy
文摘A high-throughput multi-plume pulsed-laser deposition （MPPLD） system has been demonstrated and compared to previous techniques. Whereas most combinatorial pulsedlaser deposition （PLD） systems have focused on achieving thickness uniformity using sequential multilayer deposition and masking followed by post-deposition annealing, MPPLD directly deposits a compositionally varied library of compounds using the directionality of PLD plumes and the resulting spatial variations of deposition rate. This system is more suitable for high-throughput compound thin-film fabrication.
基金supported by the Natural Science Foundation of Education Bureau of Jiangsu Province under Grant No.05KJD140035the Program for Excellent Talents in Huaiyin Teachers College
文摘We present a scheme for probabilistic remote preparation of a tripartite qutrit entangled state with a partial tripartite qutrit entangled state and a partial bipartite qutrit entangled state as the quantum channel. It is found that a bipartite qutrit orthogonal projective measurement, an auxiliary qutrit particle, and the corresponding unitary transformation are required. A scheme for probabilistic remote preparation of a tripartite qudit equatorial entangled state by using a partial tripartite qudit entangled state and a partial bipartite qudit entangled state as the quantum channel is also proposed. We calculate the successful total probability and the total classical communication cost required in the RSP process, respectively.
