Integrating lipidomics and gut microbiota to study the anti-hyperlipidemia effect of Sargentodoxae Caulis extract

Background:Sargentodoxae Caulis(SC)is the vine stem of Sargentodoxa Cuneata(Oliv.)Rehd.&E.H.Wilson in C.S.Sargent,and it in traditional Chinese medicine has been known for promoting blood circulation and removing blood stasis as recorded in the ancient book“Illustrated Classics of Materia Medica”.It has been used effectively to treat blood stasis too in modern clinical practice.However,the anti-hyperlipidemia effect of SC is not fully understood.This paper aims at exploring the use of SC stems to improve the balance of blood lipids in the body,and its new role in treating hyperlipidemia.Methods:The effects of SC extract on hyperlipidemia were explored by combining lipidomics and gut microbiota.Secondly,we explored the potential mechanism of SC in treating hyperlipidemia by pathway analysis.Results:The results showed that the stem extract of SC could restore the physiological and biochemical indices of hyperlipidemia in mice,as well as repair the morphological and structural damage to tissues.Compared to the Model group,the SC extract significantly reduced the liver index,epididymal fat index,and Lee’s index.It also significantly decreased serum levels of total cholesterol,triglycerides,low-density lipoprotein cholesterol,peroxisome proliferator-activated receptor gamma(PPAR-γ),D-lactate,and free fatty acids,while significantly increasing the relative content of peroxisome proliferator-activated receptor alpha(PPAR-α).These changes were statistically significant.Non-targeted lipidomics,based on LC-MS,were utilized to investigate the lipid metabolism characteristics in serum,liver,and epididymal fat of the subjects.It was observed that,compared to the blank group,the Model group exhibited significant changes primarily in glycerol lipids and glycerophospholipids.The treatment group also displayed alterations in these lipids.A total of 38,81,and 27 differential lipids were identified in serum,liver,and epididymal fat samples,respectively.Among these,14 common differential lipids were found in both serum and liver samples,and their KEGG enrichment pathways were largely consistent.Among them,the sphingolipid signaling pathway and the glycerophospholipid metabolic pathway were identified as key metabolic pathways that were regulated.Our gut microbiota analysis revealed that SC diminishes the abundance of Actinobacteria by altering the cecal flora in mice.Conclusion:This alteration leads to the downregulation of genes involved in triglyceride metabolism,which in turn changes lipid processing and reduces triglyceride levels.Consequently,SC effectively combats hyperlipidemia.Notably,SC impacts key metabolic pathways,including the sphingolipid signaling and glycerophospholipid metabolism.These findings underscore SC’s therapeutic potential,positioning it as a promising alternative for reducing the health risks associated with hyperlipidemia.

A new macrolide and glycosides from the stem of Sargentodoxa cuneata

A new macrolide glycoside, cuneataside F was isolated from the n-butanol extract of the stem of Sargentodoxa cuneata. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRESI-MS spectroscopic analysis.

大血藤多酚组分的化学表征及抗氧化活性研究

本实验富集大血藤多酚组分并对其进行含量测定和化学表征,丰富该药材的物质研究基础,初步分析多酚化合物体外抗氧化活性作用,为后续研究提供理论基础。采用紫外可见分光光度计确定最大吸收波长并测定总多酚含量,采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱仪对其进行化学表征,利用DPPH法和ABTS法评价大血藤总多酚的体外抗氧化活性。结果显示优化Folin-Ciocalte法最大吸收波长为740 nm,大血藤总多酚含量约为32.34%;通过高分辨质谱检测到大血藤中多酚类成分有62个,包括28个酚酸和酚苷类成分、13个木脂素类成分、12个苯丙素类成分、9个黄酮类成分;提取物对DPPH和ABTS自由基都具有清除作用,IC50值分别为4.88μg/mL、16.68μg/mL。该研究从大血藤提取物中检测到62个多酚类成分,抗氧化活性与其密切相关,深入研究了大血藤多酚组分,为该药的进一步发展提供理论基础。

基于专利数据挖掘与网络药理学探讨大血藤抗高脂血症的作用机制

[目的]采用数据挖掘和网络药理学初步探讨大血藤治疗高脂血症的可能作用机制。[方法]借助PubMed和CNKI检索“大血藤化学成分”或“红藤成分”进行资料收集和化合物筛选,用Origin Pro 2021软件对涉及中药的功效类别等进行频数分析,用SPSS Modeler 18.0软件进行关联规则分析和聚类分析。利用Swiss ADME平台来预测候选化合物的吸收度和类药性的相关参数。采用Swiss Target Prediction平台来收集预测到的所有靶点,以“hyperlipidemia”“dyslipidemia”等为关键词检索Genegards、Drug Bank Database等数据库中高脂血症的潜在靶点,通过Uniprot数据库去重和校正靶点名称,取活性成分和高脂血症靶点交集,进而运用Cytoscape 3.8.0软件来进行网络可视化并筛选核心靶点基因。通过DAVID 6.8数据库进行基因本体(GO)功能富集分析和京都基因和基因组百科全书(KEGG)通路富集分析,预测交集靶点作用机制,并绘制气泡图等进行可视化。[结果]通过数据挖掘对大血藤的用药配伍规律进行分析,结果显示性味以温性、寒性和苦味、辛味为主,归经方面以肝、脾为主,配伍以当归、甘草、红花等关联密切,研究结果与中药治疗高脂血症用药规律一致。中药-化合物-靶点网络包含了43个活性成分和相应靶点133个,关键靶点涉及类视黄酸受体α(RXRA)、信号转导及转录激活蛋白3(STAT3)、90 kDa热休克蛋白αA1(HSP90AA1)、蛋白激酶Bα(AKT1)、磷酸肌醇3-激酶调节亚基1(PIK3R1)、雌激素受体1(ESR1)、过氧化物酶体增殖物激活受体(PPAR)A、核受体亚家族3C组成员1(NR3C1)等。GO功能富集分析得到条目437个(P<0.01),KEGG通路富集分析筛选得到57条信号通路(P<0.05)。[结论]大血藤可以通过多个活性成分干预血脂异常中的多个靶点、多个环节,从多维、系统的层面参与调控血脂代谢和高脂血症的发生发展过程,为大血藤的进一步开发利用提供理论基础。

基于TCGA研究左右半结肠癌基因表达差异及苦参-大血藤-半枝莲作用机制

【目的】探讨左半结肠癌与右半结肠癌的基因表达差异及大肠癌核心药对苦参-大血藤-半枝莲作用于左右半结肠癌的机制差异。【方法】下载134例左半结肠癌及194例右半结肠癌患者癌症基因组图谱(TCGA)的转录组数据,应用R软件实现2组差异基因分析及京都基因与基因组百科全书(KEGG)通路富集分析;通过BATMAN-TCM数据库获取苦参-大血藤-半枝莲药对的活性成分及靶点,基于左右半结肠癌差异基因,分别进行药对-左/右半结肠癌KEGG富集分析、蛋白质互作(PPI)网络构建,比较药对治疗左、右半结肠癌富集的生物信号通路差异及关键靶点差异。【结果】左半结肠癌与右半结肠癌相对于正常癌旁组织共同的差异表达基因共6051个,左半结肠癌特异性的差异表达基因共1958个,右半结肠癌特异性的差异表达基因共1739个;左半结肠癌特异性KEGG富集通路14条,右半结肠癌特异性KEGG富集通路23条。苦参-大血藤-半枝莲药对活性化合物共85个,对应的靶点合计469个,药对-左半结肠癌靶点富集于10条KEGG信号通路,关键靶点为DRD2、CACNA1C、HTR3A、COMT、TH,药对-右半结肠癌靶点富集于1条KEGG信号通路,核心靶点为HTR3A、DRD2、TH、AGT、GRIN2B。【结论】左半结肠癌与右半结肠癌存在基因表达差异:左半结肠癌与免疫功能异常、AMPK信号通路异常等机制有关,右半结肠癌与中性粒细胞外陷阱形成、酒精中毒、Hippo信号通路异常等机制有关。除调控细胞周期及必需氨基酸代谢等机制外,苦参-大血藤-半枝莲药对对调节左半结肠癌肠道内分泌功能、抑制右半结肠癌炎症反应具有特异性作用,亦可能对结肠癌患者具有情绪调控作用。

Effects of “Caulis Sargentodoxae Formula” on Experimental Endometriosis in Rat

Objective To study the drug effects of ＂Caulis Sargentodoxae Formula＂ which dears away heat and blood clot on experimental endometriosis in rat, and to compare this effect with Danazol＇s effects. Method The model of endometriosis rat was induced by transplanting with endometrium surgically The rats were divided into 6 groups randomly： the non-treatment group, the castrate group, Danazol group（80 mg/kg）, ＂Caulis Sargentodoxae Formula＂ group （110 g/kg）, the medium dose group（77.5 g/kg） and the low dose group（55 g/kg）. After 3 weeks＇ treatment, the volume ofendometrium was detected by cutting the rats＇ belly open, drawing the materials from endometrium for tissue section, and doing the quantity analysis of endometrium by semiautomatic image analysis machine.Results There was no significant difference of the endometrium volumes between the low dose group and the non-treatment group（P〉O.05）, other groups＇ endometrium volumes were significantly lower than those of the non-treatment group（P〈0. 05）. And apart from the low dose group, all other groups＇ heights of the endometrium epithelia were significantly lower than that of the non-treatment group（p〈0.01）. Conclusion ＂Caulis Sargentodoxae Formula＂ can refrain the growth of the endometrium, and the effects are better than that of Danazol＇s.

Short-term Intensive Sustainable Restoration of Grasslands and Prairies Invaded with High Densities of Nitrogen-fixing Weeds： A Test with the Invasive Plant Lespedeza Cuneata

This study examines a management strategy for restoring grassland and prairie communities that have become degraded due to high density stands of invasive nitrogen-fixing plants. The novel management applications minimize the use of herbicides and maximize the competitive interactions of native species. The management method includes two seasons of application of organic fertilizer （4-1-4）, an initial herbicide （Pasture Gard, Dow Agro） application, and mowing, where mowing was a necessary treatment to control secondary growth in prairie habitats, to control high density patches of Lespedeza （L.） Cuneata, in a completely randomized factorial experiment. The herbicide was effective in reducing L. Cuneate stem density 0 stems/m^2 from an initial 88 stems/m^2 with cover reduced to 0% from 16%. The fertilizer only treatment reduced L. Cuneata percent cover to 6% from initial cover of 16%, but did not reduce the number of stems. The management strategy is an effective fast step in restoring a native prairie invaded by a nitrogen-fixing plant.

Effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients

Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.

红藤水溶性部位的LC-MS分析及抗炎活性成分的虚拟筛选

目的:基于分子对接,确定中药红藤75%乙醇提取物的水溶性部位(HT_(W))中的抗炎物质基础及相关的炎症因子。方法:采用液质联用(LC-MS)技术确定HT_(W)中的主要化学成分。采用软件Autodock Vina对化合物与肿瘤坏死因子-α、白介素-1β转化酶、IL-6、IκB激酶β、诱导性一氧化氮合酶(iNOS)及Toll样受体2(TLR2)等6种靶点蛋白的结合能进行打分,虚拟筛选出抗炎活性成分以及关键靶点。结果:通过LC-MS分析,共确定14个含量较为丰富的小分子化合物,主要为苯丙酸、苯乙醇和木脂素类化合物,其中绿原酸类化合物含量最高。分子对接结果显示,TLR2与HT_(W)中化合物的结合能平均值最高,其中与化合物2、3、7、11-13的结合能均超过-9.0 kcal/mol,远超过阈值-7.2 kcal/mol。TLR2与木通苯乙醇苷B(12)的结合能为-9.7 kcal/mol,与绿原酸(3)及其异构体(2)亦有较好的结合力。iNOS对HT_(W)中化合物也较敏感,与苯乙醇苷类化合物10的结合能为-9.8 kcal/mol。结论:绿原酸、苯乙醇苷等化合物可能是红藤水溶性部位(HT_(W))最重要的抗炎活性物质;其抗炎关键靶点可能是TLR2和iNOS。

五花血藤水煎液通过抗炎作用促进脊髓损伤后小鼠运动功能恢复

目的探讨五花血藤水煎液(Sargentodoxa cuneata,SCD)在小鼠脊髓损伤(spinal cord injury,SCI)后的抗炎作用及其对运动功能恢复的影响。方法选取36只SPF级小鼠随机分成假手术组、脊髓损伤组和SCD组,利用BMS评分检测小鼠后肢运动功能恢复情况,免疫蛋白质印迹法检测各组小鼠脊髓白细胞介素(IL)-6、IL-12A、肿瘤坏死因子(TNF)-α和脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的表达,免疫荧光染色通过NeuN观察损伤处脊髓前角神经元变化,rAAV-retro逆行示踪观察各组小鼠胫骨前肌(tibialis anterior muscle,TA)相关脊髓运动神经元分布。结果与脊髓损伤组相比,SCD组小鼠在损伤后7 d的BMS评分显著提高(P<0.05),损伤后14 d,IL-6、IL-12A和TNF-α表达明显降低(P<0.05),BDNF的表达显著升高(P<0.05);损伤处脊髓前角神经元数量显著增多(P<0.05),TA相关运动神经元数量明显增多(P<0.05)。结论五花血藤水煎液能明显下调炎症因子IL-6、IL-12A和TNF-α的表达,发挥抗炎作用,同时上调BDNF的表达从而对运动神经元起保护作用,促进损伤后小鼠运动功能的恢复。

大血藤质子核磁共振指纹图谱研究

目的建立不同产地大血藤^(1)H-NMR指纹图谱,并进行差异性分析。方法采用CPMG脉冲序列采集样品^(1)H-NMR信号,测定温度298 K,观测频率600 MHz,数据经分段积分,归一化处理后导入MetaboAnalyst 5.0软件进行统计分析。结果建立了大血藤^(1)H-NMR指纹图谱,并鉴定了13种化学成分,主成分分析、聚类分析结果表明13批大血藤药材有显著差异,方差分析结果筛选出绿原酸乙酯、正十六烷酸、红景天苷、葡萄糖、3,5-O-二甲基没食子酸、丁香醛、蔗糖、表儿茶素、绿原酸为影响大血藤质量的主要差异性成分。结论建立了大血藤^(1)H-NMR指纹图谱并筛选出差异性成分,为评价不同来源大血藤药材的质量提供了参考。

红藤汤外敷联合中药保留灌肠治疗慢性盆腔炎临床研究

目的:观察红藤汤外敷联合中药保留灌肠治疗慢性盆腔炎的临床疗效。方法:将60例慢性盆腔炎患者按照随机数字表法分为对照组和观察组,每组各30例。两组均给予常规西药治疗,对照组予以红藤汤保留灌肠,观察组在对照组的基础上联合红藤汤外敷。比较两组患者的临床疗效及治疗前后中医证候积分、妇科检查情况、炎症因子[肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、C反应蛋白(C reactive protein,CRP)、单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)、粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)]、子宫血流动力学指标[子宫搏动指数(pulsation index,PI)、阻力指数(resistance index,RI)、最大峰值流速(maximum peak flow rate,PFE)]、血液流变学指标[血浆黏度(plasma viscosity,PV)、红细胞沉降率(erythrocyte sedimentation rate,ESR)、红细胞比容(hematocrit,Hct)]变化情况。结果:两组患者治疗后中医证候积分低于本组治疗前,且治疗后观察组低于对照组,差异具有统计学意义(P<0.05)。对照组有效率为70.00%,观察组有效率为93.33%,两组患者有效率比较,差异具有统计学意义(P<0.05)。两组患者治疗后宫颈触痛、子宫活动受限、宫体/附件压痛评分、炎性包块直径、盆腔积液最深径低于本组治疗前,且治疗后观察组低于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后TNF-α、CRP、GM-CSF及MCP-1低于本组治疗前,且治疗后观察组低于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后PV、ESR及Hct低于本组治疗前,且治疗后观察组低于对照组,差异具有统计学意义(P<0.05)。结论:红藤汤外敷联合中药保留灌肠治疗慢性盆腔炎,可改善患者的临床症状,抑制炎性反应,促进血液循环。

截叶铁扫帚化学成分及药理活性研究进展

截叶铁扫帚作为民间传统中药,全草皆可入药。随着近年研究的深入,发现其具有较大的药用价值。通过对文献和图书的全面查阅,报道了截叶铁扫帚的化学成分有黄酮、酚酸、木脂素、糖、挥发油、萜类、甾体等,发现其在降脂、促胃肠动力、止咳平喘、改善肺功能、抗癌、保护男性生殖健康、抗早期动脉粥样硬化等方面有良好疗效。为充分了解截叶铁扫帚化学成分与药理活性之间的关系,现对近10年截叶铁扫帚化学成分和药理活性的研究成果进行总结和概括,以期为更好地开发和利用这一丰富的药用植物资源提供参考。

红藤败酱散加减联合氟罗沙星片治疗慢性盆腔炎临床研究

目的:观察红藤败酱散加减氟罗沙星片治疗慢性盆腔炎的临床疗效及其对患者炎性因子水平、血液流变学指标的影响。方法:将82例慢性盆腔炎患者按照随机数字表法分为观察组和对照组,每组各41例。对照组给予氟罗沙星片治疗,观察组在对照组治疗的基础上联合红藤败酱散加减。比较两组患者的临床疗效、不良反应发生率及治疗前后中医证候积分、炎性因子水平[白细胞介素(interleukin, IL)-1β、超敏C反应蛋白(hs-C reactive protein, hs-CRP)、IL-2、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)]、血液流变学指标[纤维蛋白原(fibrinogen, FIB)、血沉、全血低切黏度]变化情况。结果:两组患者治疗后中医证候积分低于本组治疗前,且治疗后观察组低于对照组(P<0.05)。观察组有效率为97.56%,高于对照组的82.93%(P<0.05)。两组患者治疗后hs-CRP、IL-1β、IL-2、TNF-α、FIB、血沉及全血低切黏度均低于本组治疗前,且治疗后观察组低于对照组(P<0.05)。观察组不良反应发生率为14.63%,对照组不良反应发生率为9.76%,组间比较,差异无统计学意义(P>0.05)。结论:红藤败酱散加减联合氟罗沙星片治疗慢性盆腔炎,可缓解患者的临床症状,降低炎性因子水平及血液流变学指标。

中药大血藤中酚类化学成分及其抗肿瘤活性

目的阐明中药大血藤中抗癌活性成分。方法以细胞周期抑制、坏死性细胞毒活性为抗癌指标 ,采用硅胶、SephadexLH 2 0、大孔树脂HP 2 0等色谱方法 ,跟踪分离活性成分 ,利用理化性质及波谱方法鉴定了化学结构 ,用SRB法及流式细胞术评价其抗癌活性。结果与结论从大血藤茎中分离得到 7个酚酸类化合物 :3,5 O 二甲基 没食子酸 (1)、原儿茶酸 (2 )、绿原酸 (3)、N (对 羟基苯乙基 )阿魏酸酰胺 (4)、对 羟基苯乙醇 (5 )、(- ) 表儿茶素 (6 )、缩合鞣质B2 (7)。化合物 3对人慢性髓性白血病K5 6 2细胞的半数抑制浓度 (IC50 )为 97 2 μg/mL ,化合物 4在 10 0 μg/mL浓度下对K5 6 2细胞的增殖抑制率为 4 6 6 %。流式细胞术检测表明 ,化合物 7对小鼠乳腺癌tsFT2 10细胞和K5 6 2细胞均显示G2 /M期抑制作用 ,为一新的细胞周期抑制剂。除化合物 2外 ,其余 6个化合物均为首次从该属植物中分离得到。

红藤饮片提取物抑菌活性与次生代谢产物含量的相关性

目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。

不同海拔高度大血藤群体遗传多样性的RAPD分析及其与环境因子的相关性

利用 RAPD技术分析了分布于浙江省天台山 3个不同海拔高度的天然大血藤群体的遗传多样性、遗传分化以及与环境因子的相关性。 13种随机引物在 3 6株个体中共检测到 88个可重复的位点 ,其中多态位点 74个 ,总多态位点百分率为84.0 9% ,大血藤具有丰富的遗传多样性。 Shannon信息指数显示的遗传多样性以海拔 950 m的群体为最高 ,其次是海拔 73 0 m的群体 ,最低的是海拔 52 0 m的群体 ;群体内的遗传多样性占总遗传多样性的 43 .68% ,群体间的遗传多样性占 56.3 2 %。 Nei指数估计大血藤群体间的遗传分化系数为 0 .540 6,大血藤群体间的基因流很低。大血藤海拔 73 0 m群体与海拔 52 0 m群体的遗传相似度较高 ,海拔 950 m群体与其它两群体的遗传相似度较低。大血藤群体内的遗传多样性与土壤总氮呈极显著的正相关。

大血藤黄酮类化合物组成的初步研究

大血藤为一种传统药用植物。为了了解大血藤的药用成分,利用聚酰胺薄层层析及HPLC法分析了大血藤各器官黄酮类化合物的组成。聚酰胺薄层层析及HPLC法分析都显示大血藤叶片含有10种不同的黄酮甙,而嫩茎、叶柄与老茎中分别含有10种、8种及5种不同的黄酮甙;大血藤叶片黄酮类化合物经盐酸水解后HPLC分析显示有8种不同的黄酮类化合物。大血藤叶片及嫩茎的黄酮类化合物组成成分较多,具有一定的应用开发前景。

大血藤DNA提取及RAPD研究初探

以浙江天台山大血藤为材料 ,对其DNA提取和RAPD分子标记方法进行了研究。结果表明 :所采用的经改进的SDS提取法可获得高质量的大血藤DNA ,分子量大于 2 3kb ,可满足RPAD扩增 ;用 1 5个不同的随机引物对所提取的 1 2个个体的大血藤DNA进行了RAPD分子标记分析 ,其中 1 4个引物扩增产物具有多态性。建立了大血藤DNA提取和RAPD标记的分析程序 。

大孔树脂法分离纯化大血藤中原花青素

采用大孔树脂法提取和纯化大血藤中原花青素,以吸附及解吸附能力为指标,比较D101、HPD100、X5、AB8、及ADS17五种大孔树脂对原花青素的吸附效率,通过单因素实验考察上样流速、上样浓度、洗脱流速、洗脱剂用量及洗脱剂体积分数对提取原花青素含量的影响,优选树脂的动态吸附及解吸附条件,并评价提取得到原花青素的纯度。结果表明,HPD100树脂对大血藤中原花青素的吸附和解吸附效果最好,上样流速2 BV/h,上样浓度6 mg/m L,洗脱流速1 BV/h,洗脱剂用量2 BV的纯化效果最好,100%乙醇的洗脱量最大,得到原花青素的纯度是粗提物的1.76倍。