Products of plant resistance ( R ) genes Pto and Cf contain distinct domains, and have different cellular localization. It is intriguing to compare the development mechanisms of resistance conferred by the t...Products of plant resistance ( R ) genes Pto and Cf contain distinct domains, and have different cellular localization. It is intriguing to compare the development mechanisms of resistance conferred by the two R genes. In the present report, two hypersensitive response (HR) initiation systems were employed to study the time_course expression induced by Avr / Cf interaction of the genes encoding Pti4, Pti5 and Pti6 which interact directly with Pto: (1) Seeds of tomato (Lycopersicon esculentum Mill.) containing complementary gene pairs Avr 4/ Cf _4 and Avr 9/ Cf _9 were obtained through crossing. Their seedlings developed HR under room temperature. (2) Avr / Cf seedlings grew normally at 33 ℃. When the temperature was shifted down to 25 ℃, HR occurred within hours in the seedlings. Results of both experiments showed that expression of Pti4, Pti5 and Pti6 was induced upon development of hypersensitive necrosis in Avr / Cf seedlings. However, the expression levels and patterns of these Pti s differed. This finding indicated that these Pti s function complementarily, and might be involved in regulation of both Pto and Cf _conferred resistance.展开更多
番茄Pto基因是一类可以编码丝氨酸/苏氨酸激酶(STK)序列的广谱抗性候选基因,其序列克隆与鉴定为深入了解番茄的抗病机制奠定了基础。在该研究中,一对依据Pto基因的保守序列设计的简并引物被用来扩增巴西橡胶中Pto基因抗病同源序列,扩增...番茄Pto基因是一类可以编码丝氨酸/苏氨酸激酶(STK)序列的广谱抗性候选基因,其序列克隆与鉴定为深入了解番茄的抗病机制奠定了基础。在该研究中,一对依据Pto基因的保守序列设计的简并引物被用来扩增巴西橡胶中Pto基因抗病同源序列,扩增得到了一个约550 bp的基因片段,其随后被克隆并测序。序列分析发现,其中的7个抗病同源序列与Pto基因高度同源(BLASTX E value<3e-53),所以其被认为是Pto基因抗病同源序列(Pto-RGCs)。通过巴西橡胶的Pto-RGCs多序列比对表明,这些序列包含了多个STKs保守的次级结构域。此外,系统发育分析也表明,巴西橡胶的Pto-RGCs属于Pto基因同源的R基因。该研究结果中Pto-RGCs可为巴西橡胶抗病的发展提供一个有效的基因资源。展开更多
文摘Products of plant resistance ( R ) genes Pto and Cf contain distinct domains, and have different cellular localization. It is intriguing to compare the development mechanisms of resistance conferred by the two R genes. In the present report, two hypersensitive response (HR) initiation systems were employed to study the time_course expression induced by Avr / Cf interaction of the genes encoding Pti4, Pti5 and Pti6 which interact directly with Pto: (1) Seeds of tomato (Lycopersicon esculentum Mill.) containing complementary gene pairs Avr 4/ Cf _4 and Avr 9/ Cf _9 were obtained through crossing. Their seedlings developed HR under room temperature. (2) Avr / Cf seedlings grew normally at 33 ℃. When the temperature was shifted down to 25 ℃, HR occurred within hours in the seedlings. Results of both experiments showed that expression of Pti4, Pti5 and Pti6 was induced upon development of hypersensitive necrosis in Avr / Cf seedlings. However, the expression levels and patterns of these Pti s differed. This finding indicated that these Pti s function complementarily, and might be involved in regulation of both Pto and Cf _conferred resistance.
基金Supported by the National Natural Science Foundation of China(81374065)Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences-Tropical Crops Genetic Resources Institute(1630032015020)Science and Technology Park Construction Funds of Hainan~~
文摘番茄Pto基因是一类可以编码丝氨酸/苏氨酸激酶(STK)序列的广谱抗性候选基因,其序列克隆与鉴定为深入了解番茄的抗病机制奠定了基础。在该研究中,一对依据Pto基因的保守序列设计的简并引物被用来扩增巴西橡胶中Pto基因抗病同源序列,扩增得到了一个约550 bp的基因片段,其随后被克隆并测序。序列分析发现,其中的7个抗病同源序列与Pto基因高度同源(BLASTX E value<3e-53),所以其被认为是Pto基因抗病同源序列(Pto-RGCs)。通过巴西橡胶的Pto-RGCs多序列比对表明,这些序列包含了多个STKs保守的次级结构域。此外,系统发育分析也表明,巴西橡胶的Pto-RGCs属于Pto基因同源的R基因。该研究结果中Pto-RGCs可为巴西橡胶抗病的发展提供一个有效的基因资源。
