Lake is an important part of the natural ecosystem, and its morphological characteristics reflect the capacity of lake regulation and storage, the strength of material migration, and the characteristics of shoreline d...Lake is an important part of the natural ecosystem, and its morphological characteristics reflect the capacity of lake regulation and storage, the strength of material migration, and the characteristics of shoreline development. In most existing studies, remote sensing images are used to quantify the morphological characteristics of lakes. However, the extraction accuracy of lake water is greatly affected by cloud cover and vegetation cover, and the inversion accuracy of lake elevation data is poor, which cannot accurately describe the response relationship of lake landscape morphology with water level change. Therefore, this paper takes Tonle Sap Lake as the research object, which is the largest natural freshwater lake in Southeast Asia. DEM is constructed based on high-resolution measured topographic data, and morphological indicators such as lake area, lake shoreline length, perimeter area ratio, longest axis length, maximum width, shoreline development index, lake shape complexity, compactness ratio and form ratio are adopted to researching the evolution law of high water overflows and low water outbursts quantitatively, and clarifying the variation characteristics of landscape morphology with water level gradient in Tonle Sap Lake. The research results have important theoretical significance for the scientific utilization of Tonle Sap Lake water resources and the protection of the lake ecosystem.展开更多
S-SAP(特异序列扩增多态性,Ssequence specific amplification polymorphism)是一种基于反转录转座元件的广泛应用于生物遗传多样性研究的分子标记。本研究从34对引物中筛选出7对具有谱带清晰、多态性高的引物组合,成功地开发了苹果基...S-SAP(特异序列扩增多态性,Ssequence specific amplification polymorphism)是一种基于反转录转座元件的广泛应用于生物遗传多样性研究的分子标记。本研究从34对引物中筛选出7对具有谱带清晰、多态性高的引物组合,成功地开发了苹果基因组的S-SAP分子标记。27个元帅系芽变品种中,共扩增出588条谱带,每对引物组合平均扩增出84条谱带,其中多态性谱带48条,多态性谱带占总扩增出谱带数的8.2%,遗传相似系数介于0.73～0.90之间。对15个苹果芽变品种进行S-SAP分析,相似系数在0.42～0.94之间,以相似系数0.80为阈值,可以区分各芽变品系。开发的S-SAP分子标记可以有效地将苹果芽变品种区分,并为苹果生物遗传多样性与系统进化、品种鉴定提供新方法。展开更多
文摘Lake is an important part of the natural ecosystem, and its morphological characteristics reflect the capacity of lake regulation and storage, the strength of material migration, and the characteristics of shoreline development. In most existing studies, remote sensing images are used to quantify the morphological characteristics of lakes. However, the extraction accuracy of lake water is greatly affected by cloud cover and vegetation cover, and the inversion accuracy of lake elevation data is poor, which cannot accurately describe the response relationship of lake landscape morphology with water level change. Therefore, this paper takes Tonle Sap Lake as the research object, which is the largest natural freshwater lake in Southeast Asia. DEM is constructed based on high-resolution measured topographic data, and morphological indicators such as lake area, lake shoreline length, perimeter area ratio, longest axis length, maximum width, shoreline development index, lake shape complexity, compactness ratio and form ratio are adopted to researching the evolution law of high water overflows and low water outbursts quantitatively, and clarifying the variation characteristics of landscape morphology with water level gradient in Tonle Sap Lake. The research results have important theoretical significance for the scientific utilization of Tonle Sap Lake water resources and the protection of the lake ecosystem.
文摘S-SAP(特异序列扩增多态性,Ssequence specific amplification polymorphism)是一种基于反转录转座元件的广泛应用于生物遗传多样性研究的分子标记。本研究从34对引物中筛选出7对具有谱带清晰、多态性高的引物组合,成功地开发了苹果基因组的S-SAP分子标记。27个元帅系芽变品种中,共扩增出588条谱带,每对引物组合平均扩增出84条谱带,其中多态性谱带48条,多态性谱带占总扩增出谱带数的8.2%,遗传相似系数介于0.73～0.90之间。对15个苹果芽变品种进行S-SAP分析,相似系数在0.42～0.94之间,以相似系数0.80为阈值,可以区分各芽变品系。开发的S-SAP分子标记可以有效地将苹果芽变品种区分,并为苹果生物遗传多样性与系统进化、品种鉴定提供新方法。
