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应用PCR-DGGE技术分析黄海冷水团海域的细菌群落组成 被引量:29
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作者 刘敏 朱开玲 +2 位作者 李洪波 张涛 肖天 《环境科学》 CAS CSCD 北大核心 2008年第4期1082-1091,共10页
利用PCR-DGGE技术对2个季节(2006-04和2006-10)的黄海冷水团海域的细菌群落组成和优势菌群进行了分析.通过软件Glyko Bandscan分析DGGE图谱,4月份所有站位的条带数相近(约17条),多样性丰富;10月份,在冷水团范围以内站位的条带约16条... 利用PCR-DGGE技术对2个季节(2006-04和2006-10)的黄海冷水团海域的细菌群落组成和优势菌群进行了分析.通过软件Glyko Bandscan分析DGGE图谱,4月份所有站位的条带数相近(约17条),多样性丰富;10月份,在冷水团范围以内站位的条带约16条,其多样性也较丰富;而在冷水团范围以外站位的条带少(约12条),其多样性较低.细菌16S rDNA V3区特征片段经DGGE分离、条带切割,共得到24条条带,克隆、测序后,进行系统进化分析,结果显示这24条带分别归属于2个细菌类群:变形细菌门(Proteobacteria)和拟杆菌门(Bacteroides).在24条序列中有16条分别与变形细菌亚群的γ-和δ-Proteobacteria相似,有5条与拟杆菌门相似.时空分析发现,4月份所有站位水体(海水温度为7-12℃)的细菌群落组成和10月份(冷水团存在期)冷水团内部水体(海水温度低于10℃)的细菌群落组成相同(都包括γ-Proteobacteria、δ-Proteobacteria和Bacteroides),与10月份冷水团外部水体(海水温度大于19℃)的(包括γ-Proteobacteria和Bacteroides)不同.优势菌群也存在同样的分布特点,4月份所有站位水体的优势菌群与10月份冷水团内部水体的优势菌群也相同(都是γ-Proteobacteria),而10月份冷水团外部水体不同的站位优势菌群不同.该海域细菌群落组成和优势菌群的分布特点,可能与冷水团的形成有一定的相关性. 展开更多
关键词 细菌群落组成 16SrDNA序列分析 PCR-DGGE 黄海冷水团
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黄海冷水团新生产力及微食物环作用分析 被引量:9
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作者 李杰 吴增茂 万小芳 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2006年第2期193-199,共7页
为了进一步揭示黄海冷水团水域浮游生态系统能流、物流循环的基本动力学特征,利用水层-底栖耦合生态系统垂直3层模型,模拟分析了该水域生源要素的基本特征及年变化特点,并着重分析了初级生产力、新生产力及微食物环作用。通过对黄海冷... 为了进一步揭示黄海冷水团水域浮游生态系统能流、物流循环的基本动力学特征,利用水层-底栖耦合生态系统垂直3层模型,模拟分析了该水域生源要素的基本特征及年变化特点,并着重分析了初级生产力、新生产力及微食物环作用。通过对黄海冷水团水域初级生产力和新生产力的模拟分析,估算出黄海冷水团年平均初级生产力为228.47mg.m-2.d-1,并根据模型中营养盐的来源估算出f比约为33%。分析模拟结果还表明,在黄海冷水团存在的季节,冷水团水域水层营养盐匮乏,浮游植物生产受限制,仅在晚秋出现了不十分明显的“秋华”。然而,在此时段内,浮游动物的生物量基本和浮游植物为同一量级且量值比较接近,由此可推断微食物环在黄海冷水团能流、物流循环过程中担当了重要的角色。模拟结果表明,浮游动物生长所需的碳约有60%是通过微食物环供给的。与实测及文献结果相比较,模型的模拟结果是合理可信的。 展开更多
关键词 黄海冷水团 水层-底栖耦合生态系统 初级生产力 f比 新生产力 微食物环
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Chemicohydrographic characteristics of the Yellow Sea Cold Water Mass 被引量:12
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作者 XIN Ming MA Deyi WANG Baodong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2015年第6期5-11,共7页
Based on the field data obtained during summer cruises in 2006, the overall perspective of chemical and hydrographic characteristics of the Yellow Sea Cold Water Mass (YSCWM) are discussed through the cross- YSCWM t... Based on the field data obtained during summer cruises in 2006, the overall perspective of chemical and hydrographic characteristics of the Yellow Sea Cold Water Mass (YSCWM) are discussed through the cross- YSCWM transect profiles and horizontal distributions of hydrological and chemical variables, with emphasis on the differences between the northern Yellow Sea Cold Water Mass (NYSCWM) and the southern Yellow Sea Cold Water Mass (SYSCWM). The results show that YSCWM is characterized by low temperature (〈10℃) and dissolved oxygen (DO) concentration, high salinity (〉32.0) and nutrient concentrations. Compared to the SYSCWM, the NYSCWM possesses lower values of temperature, salinity and nutrient concentrations but higher values of DO. Also its smaller variation ranges of variables (except for temperature) demonstrate that NYSCWM is more uniform than that of SYSCWM. In addition, thermocline is more intensive in the SYSCWM than that of NYSCWM. Furthermore, DO and Chl a maxima appear at the depth of 30 m in the SYSCWM, while these phenomena are not obvious in the NYSCWM. 展开更多
关键词 Yellow Sea Cold Water Mass (yscwm) horizontal distribution vertical profile chemicohydrographic characteristics
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Long-term variability of the sharp thermocline in the Yellow and East China Seas 被引量:3
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作者 郝佳佳 陈永利 王凡 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第6期1016-1025,共10页
Based on observed temperature data since the 1950s, long-term variability of the summer sharp thermocline in the Yellow Sea Cold Water Mass (YSCWM) and East China Sea Cold Eddy (ECSCE) areas is examined. Relations... Based on observed temperature data since the 1950s, long-term variability of the summer sharp thermocline in the Yellow Sea Cold Water Mass (YSCWM) and East China Sea Cold Eddy (ECSCE) areas is examined. Relationships between the thermocline and atmospheric and oceanic forcing were investigated using multiyear wind, Kuroshio discharge and air temperature data. Results show that: 1) In the YSCWM area, thermocline strength shows about 4-year and 16-year period oscillations. There is high correlation between summer thermocline strength and local atmospheric temperature in summer and the previous winter; 2) In the ECSCE area, interannual oscillation of thermocline strength with about a 4-year period (stronger in El Nifio years) is strongly correlated with that of local wind stress. A transition from weak to strong thermocline during the mid 1970s is consistent with a 1976/1977 climate shift and Kuroshio volume transport; 3) Long-term changes of the thermocline in both regions are mainly determined by deep layer water, especially on the decadal timescale. However, surface water can modify the thermocline on an interannual timescale in the YSCWM area. 展开更多
关键词 THERMOCLINE long-term variability ECS Cold Eddy (ECSCE) YS Cold Water Mass (yscwm)
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Annual variation in Calanus sinicus abundance and population structure in the northern boundary area of the Yellow Sea Cold Water Mass 被引量:2
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作者 尹洁慧 张光涛 +2 位作者 赵增霞 王世伟 万艾勇 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2013年第6期1284-1294,共11页
The Yellow Sea Cold Water Mass (YSCWM) was suggested as an over-summering site of the dominant copepod species Calanus sinicus in coastal Chinese seas. Population abundance and structure were investigated by monthly... The Yellow Sea Cold Water Mass (YSCWM) was suggested as an over-summering site of the dominant copepod species Calanus sinicus in coastal Chinese seas. Population abundance and structure were investigated by monthly sampling along three transects across the northern boundary of the YSCWM during 2009-2010. Results show that thermal stratification existed from June to October and that the vertical thermal difference increased with depth. Generally, total abundance was lowest in October and highest in June, and the female/male sex ratio was highest in February and lowest in August. Evident spatial differences in abundance were observed during the existence of the YSCWM. In June, total abundance averaged 158.8 ind/m~ at well-stratified stations, and 532.1 ind/m3 at other stations. Similarly, high abundances of 322.0 and 324.4 ind/m3 were recorded from July to August inside the YSCWM, while the abundance decreased from 50.4 to 1.9 ind/m3 outside the water mass. C. sinicus distribution tended to even out over the study area in September when the YSCWM disappeared. We believe that the YSCWM may retard population recruitment in spring and preserve abundant cohorts in summer. The summer population was transported to neritic waters in autumn. In addition to low temperatures, stable vertical structure was also an essential condition for preservation of the summer population. C. sinicus can survive the summer in marginal areas in high abundance, but the population structure is completely different in terms of C5 proportion and sex ratio. 展开更多
关键词 Calanus sinicus Yellow Sea Cold Water Mass (yscwm) over-summer strategy boundary area thermal stratification
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黄海冷水团可培养细菌的多样性研究
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作者 王红 刘吉文 张晓华 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第6期61-67,共7页
本文研究了2008年7月黄海冷水团海域可培养细菌的多样性。AODC和DVC计数总菌数和活菌数分别为(1.34.8)×105/mL和(0.61.6)×105/mL;2216E平板以菌落计数法可培养细菌浓度为(0.562.2)×103cfu/mL。从分离到的475株中选... 本文研究了2008年7月黄海冷水团海域可培养细菌的多样性。AODC和DVC计数总菌数和活菌数分别为(1.34.8)×105/mL和(0.61.6)×105/mL;2216E平板以菌落计数法可培养细菌浓度为(0.562.2)×103cfu/mL。从分离到的475株中选取163株进行16S rDNA扩增,并用HhaⅠ酶进行ARDRA(扩增性rDNA限制酶切片段分析)多态性分析,取114株不同带型测序。结果显示,冷水团细菌归为4个细菌类群:变形菌门(Proteobacteria)、放线菌门(Acti-nobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes),共24个属,其中包括α-,β-,γ-变形菌纲;非冷水团细菌也归为这4个门类,共15个属,但未分离到β-变形菌纲。γ-变形菌纲在冷水团和非冷水团区域的不同深度都是优势菌群,不同的是冷水团区域α-变形菌纲比例较高(26.8%),而非冷水团区域α-变形菌纲比例相对较低(15.6%)。这表明黄海冷水团和非冷水团区域细菌多样性都很丰富,但其群落组成和优势菌群有所不同。此外,16S rDNA测序结果表明,6株细菌可能为海洋细菌新种。 展开更多
关键词 可培养细菌 多样性 16S rDNA分析 黄海冷水团
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南黄海-长江口海域溶解氧分布和低氧、酸化特征及其控制因素 被引量:5
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作者 吴林妮 韦钦胜 +2 位作者 冉祥滨 孙俊川 王保栋 《中国环境科学》 EI CAS CSCD 北大核心 2021年第3期1311-1324,共14页
基于2019年8月与11月所获取的2个航次的水文、化学和生物资料,重点研究了夏、秋季南黄海和长江口海域溶解氧(DO)分布的时空格局及其与水文动力状况和生物地球化学过程之间的关系,探讨了该海区低氧特征及其对酸化环境的指示.结果表明,研... 基于2019年8月与11月所获取的2个航次的水文、化学和生物资料,重点研究了夏、秋季南黄海和长江口海域溶解氧(DO)分布的时空格局及其与水文动力状况和生物地球化学过程之间的关系,探讨了该海区低氧特征及其对酸化环境的指示.结果表明,研究海域内DO含量及分布与流场/水团格局具有良好的对应关系,其中夏季长江口外台湾暖流影响区具有低氧的特征,秋季则在黄海冷水团海域下底层存在一个DO低值区.在上升流影响下,夏季长江口海域的底层低氧水体可抬升至上层水体,低氧水体由长江口海域向东北方向扩展;夏季长江口外下层低氧水体的涌升、黄海冷水团区DO最大值层的抬升均与跃层的抬升趋势基本一致,不仅反应了上升流对DO垂向分布的影响,同时也指示了冷水团边界区和长江口外海域涌升流的存在.此外,夏季长江口外东北部离岸低盐水团区的生物地球化学过程也对局地底层低氧区的形成有重要贡献.在夏季的长江口海域和秋季的黄海冷水团海域存在水体酸化区,其中长江口的酸化区范围与低氧区总体相吻合,秋季黄海冷水团底层酸化区也与DO低值区和冷水团范围基本一致.本研究结果可为南黄海和长江口海域低氧和酸化等生态环境问题的改善提供理论指导. 展开更多
关键词 溶解氧(DO) 低氧区 酸化 上升流 黄海冷水团 长江口
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