子痫前期是一种妊娠特异性疾病,是孕产妇和围生期死亡的主要原因。至今尚无任何一种假说可以彻底地解释子痫前期的病理变化,目前比较公认的特征是早期胎盘滋养细胞浸润和螺旋动脉重铸减少。ACVR2A(activin A receptor type 2A)是转化生...子痫前期是一种妊娠特异性疾病,是孕产妇和围生期死亡的主要原因。至今尚无任何一种假说可以彻底地解释子痫前期的病理变化,目前比较公认的特征是早期胎盘滋养细胞浸润和螺旋动脉重铸减少。ACVR2A(activin A receptor type 2A)是转化生长因子β(TGF-β)受体家族的成员,被认为是生殖功能的重要调节因子,尤其是在蜕膜化、滋养细胞浸润和胎盘形成过程中起重要作用。近些年的研究表明激活素A及其受体在子痫前期的发生、发展过程中有重要的调控作用。综述ACVR2A与子痫前期的关系,旨在为子痫前期的发病机制、早期诊断及治疗提供新的理论依据。展开更多
旨在探讨波尔山羊激活素受体ⅡB(activin A receptor typeⅡB,ACVRⅡB)基因5′调控区多态性及其对初生体重体尺的影响。从103只波尔山羊耳组织中提取基因组DNA,扩增ACVRⅡB基因5′调控区序列,测序鉴定SNPs位点,分析遗传多态性,并与波尔...旨在探讨波尔山羊激活素受体ⅡB(activin A receptor typeⅡB,ACVRⅡB)基因5′调控区多态性及其对初生体重体尺的影响。从103只波尔山羊耳组织中提取基因组DNA,扩增ACVRⅡB基因5′调控区序列,测序鉴定SNPs位点,分析遗传多态性,并与波尔山羊初生体重、体尺进行关联分析。结果显示,鉴定到g.-1776C>G、g.-1725G>A、g.-1697C>T、g.-1604G>A、g.-1569G>C 5个SNPs位点,其中g.-1725G>A位点AA型在群体中频率为0.049,AG型为0.359,GG型为0.592,PIC为0.29,处于中度多态;g.-1569G>C位点CG型在群体中频率为0.167,GG型为0.833,PIC为0.14,处于低度多态。连锁分析发现g.-1725 G>A与g.-1697C>T、g.-1569G>C完全连锁(相关性为100%)。关联分析发现,g.-1725G>A位点AG型初生重、初生体长均显著高于GG型(P<0.05);g.-1569G>C位点CG型初生体高显著高于GG型。综上,g.-1725G>A位点可作为波尔山羊早期生长选育的候选分子标记。展开更多
Activin A receptor,type II(Acvr2)is a member of the transforming growth factor beta receptor family and can function as a negative regulator of skeletal muscle mass.Acvr2 plays an important role in regulating muscle d...Activin A receptor,type II(Acvr2)is a member of the transforming growth factor beta receptor family and can function as a negative regulator of skeletal muscle mass.Acvr2 plays an important role in regulating muscle development that can inhibit skeletal muscle growth in mice.However,there is very little research reported on the function of acvr2 in muscle development of teleost.In this study,we analyzed the effect of acvr2aa and acvr2ba on muscle development in zebrafish.Growth rates of WT and acvr2a^(-/-b-/-)were measured from juvenile stage to adult stage.In addition,effects of acvr2 on skeletal muscle were tested in histological,protein and molecular levels.As a result,acvr2a^(-/-b-/-)exhibited a wider body trunk than WT and showed a significant increase in body weight and width from two months old.Histological analysis of skeletal muscle indicated that the size of muscle fiber in acvr2a^(-/-b-/-)(female:1809±123μm^(2);male:2261±130μm^(2))was larger than that in WT(709.8±49μm^(2);815±53μm^(2)).In addition,western blot of fast MyLc protein showed the protein synthesis of acvr2a^(-/-b-/-)are increased.Besides,Histological analysis of heart showed the ventricle area is aslo increased in acvr2a^(-/-b-/-).Our results demonstrated acvr2 attends the development of muscle fiber and will cause muscle hypertrophy when they were knocked out in zebrafish.In conclusion,acvr2 in zebrafish can control the development of muscle fibers during posthatch growth.展开更多
Background: MicroRNAs act as post-transcriptional regulators that repress translation or degrade mRNA transcripts.Each microRNA has many mRNA targets and each mRNA may be targeted by several microRNAs. Skeletal muscle...Background: MicroRNAs act as post-transcriptional regulators that repress translation or degrade mRNA transcripts.Each microRNA has many mRNA targets and each mRNA may be targeted by several microRNAs. Skeletal muscles express a plethora of microRNA genes that regulate muscle development and function by controlling the expression of protein-coding target genes. To expand our understanding of the role of microRNA, specifically btamiR-365-3 p, in muscle biology, we investigated its functions in regulating primary bovine myoblast proliferation and differentiation.Results: Firstly, we found that bta-miR-365-3 p was predominantly expressed in skeletal muscle and heart tissue in Chinese Qinchuan beef cattle. Quantitative PCR and western blotting results showed that overexpression of btamiR-365-3 p significantly reduced the expression levels of cyclin D1(CCND1), cyclin dependent kinase 2(CDK2) and proliferating cell nuclear antigen(PCNA) but stimulated the expression levels of muscle differentiation markers, i.e.,MYOD1, MYOG at both mRNA and protein level. Moreover, downregulation of bta-miR-365-3 p increased the expression of CCND1, CDK2 and PCNA but decreased the expression of MYOD1 and MYOG at both mRNA and protein levels. Furthermore, flow cytometry, EdU proliferation assays and immunostaining results showed that increased levels of bta-miR-365-3 p suppressed cell proliferation but promoted myotube formation, whereas decreased levels of bta-miR-365-3 p resulted in the opposite consequences. Finally, we identified that activin A receptor type I(ACVR1) could be a direct target of bta-miR-365-3 p. It was demonstrated that bta-miR-365-3 p can bind to the 3'UTR of ACVR1 gene to regulate its expression based on dual luciferase gene reporter assays.Consistently, knock-down of ACVR1 was associated with decreased expressions of CDK2, CCND1 and PCNA but increased expression of MYOG and MYOD1 both at mRNA and protein level.Conclusion: Collectively, these data suggested that bta-miR-365-3 p represses proliferation but promotes differentiation of bovine myoblasts through several biological mechanisms involving downregulation of ACVR1.展开更多
文摘子痫前期是一种妊娠特异性疾病,是孕产妇和围生期死亡的主要原因。至今尚无任何一种假说可以彻底地解释子痫前期的病理变化,目前比较公认的特征是早期胎盘滋养细胞浸润和螺旋动脉重铸减少。ACVR2A(activin A receptor type 2A)是转化生长因子β(TGF-β)受体家族的成员,被认为是生殖功能的重要调节因子,尤其是在蜕膜化、滋养细胞浸润和胎盘形成过程中起重要作用。近些年的研究表明激活素A及其受体在子痫前期的发生、发展过程中有重要的调控作用。综述ACVR2A与子痫前期的关系,旨在为子痫前期的发病机制、早期诊断及治疗提供新的理论依据。
基金the National Natural Science Foundation of China[31872546].
文摘Activin A receptor,type II(Acvr2)is a member of the transforming growth factor beta receptor family and can function as a negative regulator of skeletal muscle mass.Acvr2 plays an important role in regulating muscle development that can inhibit skeletal muscle growth in mice.However,there is very little research reported on the function of acvr2 in muscle development of teleost.In this study,we analyzed the effect of acvr2aa and acvr2ba on muscle development in zebrafish.Growth rates of WT and acvr2a^(-/-b-/-)were measured from juvenile stage to adult stage.In addition,effects of acvr2 on skeletal muscle were tested in histological,protein and molecular levels.As a result,acvr2a^(-/-b-/-)exhibited a wider body trunk than WT and showed a significant increase in body weight and width from two months old.Histological analysis of skeletal muscle indicated that the size of muscle fiber in acvr2a^(-/-b-/-)(female:1809±123μm^(2);male:2261±130μm^(2))was larger than that in WT(709.8±49μm^(2);815±53μm^(2)).In addition,western blot of fast MyLc protein showed the protein synthesis of acvr2a^(-/-b-/-)are increased.Besides,Histological analysis of heart showed the ventricle area is aslo increased in acvr2a^(-/-b-/-).Our results demonstrated acvr2 attends the development of muscle fiber and will cause muscle hypertrophy when they were knocked out in zebrafish.In conclusion,acvr2 in zebrafish can control the development of muscle fibers during posthatch growth.
基金supported by the National Natural Science Foundation of China (No.31772574)the Program of National Beef Cattle and Yak Industrial Technology System (CARS-37)the scholarship from the China Scholarship Council (CSC),China。
文摘Background: MicroRNAs act as post-transcriptional regulators that repress translation or degrade mRNA transcripts.Each microRNA has many mRNA targets and each mRNA may be targeted by several microRNAs. Skeletal muscles express a plethora of microRNA genes that regulate muscle development and function by controlling the expression of protein-coding target genes. To expand our understanding of the role of microRNA, specifically btamiR-365-3 p, in muscle biology, we investigated its functions in regulating primary bovine myoblast proliferation and differentiation.Results: Firstly, we found that bta-miR-365-3 p was predominantly expressed in skeletal muscle and heart tissue in Chinese Qinchuan beef cattle. Quantitative PCR and western blotting results showed that overexpression of btamiR-365-3 p significantly reduced the expression levels of cyclin D1(CCND1), cyclin dependent kinase 2(CDK2) and proliferating cell nuclear antigen(PCNA) but stimulated the expression levels of muscle differentiation markers, i.e.,MYOD1, MYOG at both mRNA and protein level. Moreover, downregulation of bta-miR-365-3 p increased the expression of CCND1, CDK2 and PCNA but decreased the expression of MYOD1 and MYOG at both mRNA and protein levels. Furthermore, flow cytometry, EdU proliferation assays and immunostaining results showed that increased levels of bta-miR-365-3 p suppressed cell proliferation but promoted myotube formation, whereas decreased levels of bta-miR-365-3 p resulted in the opposite consequences. Finally, we identified that activin A receptor type I(ACVR1) could be a direct target of bta-miR-365-3 p. It was demonstrated that bta-miR-365-3 p can bind to the 3'UTR of ACVR1 gene to regulate its expression based on dual luciferase gene reporter assays.Consistently, knock-down of ACVR1 was associated with decreased expressions of CDK2, CCND1 and PCNA but increased expression of MYOG and MYOD1 both at mRNA and protein level.Conclusion: Collectively, these data suggested that bta-miR-365-3 p represses proliferation but promotes differentiation of bovine myoblasts through several biological mechanisms involving downregulation of ACVR1.
