在第54届美国质谱学会年会上,Waters公司发布了全球第一款可通过质量、尺寸和形状来区分样品离子的质谱技术,Waters Synapt^TM High Definition MS^TM(HDMS^TM)系统。这一技术的问世,将突破传统质谱的极限,可对复杂样品进行更为...在第54届美国质谱学会年会上,Waters公司发布了全球第一款可通过质量、尺寸和形状来区分样品离子的质谱技术,Waters Synapt^TM High Definition MS^TM(HDMS^TM)系统。这一技术的问世,将突破传统质谱的极限,可对复杂样品进行更为深入的表征和鉴定。展开更多
A new semantic model in Abstract State Model (ASM) for authentication protocols is presented. It highlights the Woo-Lam's ideas for authentication, which is the strongest one in Lowe's definition hierarchy for...A new semantic model in Abstract State Model (ASM) for authentication protocols is presented. It highlights the Woo-Lam's ideas for authentication, which is the strongest one in Lowe's definition hierarchy for entity authentication. Apart from the flexible and natural features in forming and analyzing protocols inherited from ASM, the model defines both authentication and secrecy properties explicitly in first order sentences as invariants. The process of proving security properties with respect to an authentication protocol blends the correctness and secrecy properties together to avoid the potential flaws which may happen when treated separately. The security of revised Helsinki protocol is shown as a case study. The new model is different from the previous ones in ASMs.展开更多
Background:Nonalcoholic fatty liver disease(NAFLD)is a global health concern with the acid sphingomyelinase(ASM)/ceramide(CE)pathway and the NOD-like receptor family,pyrin domain-containing protein 3(NLRP3)inflammasom...Background:Nonalcoholic fatty liver disease(NAFLD)is a global health concern with the acid sphingomyelinase(ASM)/ceramide(CE)pathway and the NOD-like receptor family,pyrin domain-containing protein 3(NLRP3)inflammasome identified as pivotal players in lipid disorders and inflammation.This study explores the interaction mechanism between the ASM/CE pathway and NLRP3 in NAFLD cell models,aiming to understand the impact of amitriptyline(Ami),an ASM inhibitor,on lipid deposition and hepatocyte injury by regulating the ASM/CE-NLRP3 pathway.Methods:HepG2 and HL-7702 cells were exposed to free fatty acids(FFAs)to establish the NAFLD model.The cells were divided into 5 groups:control group,model group,Ami group,tumor necrosis factoralpha(TNF-α)group,and Ami+TNF-αgroup.Intracellular lipid droplets were visualized using Oil Red O staining,and Western blot analysis quantified ASM,NLRP3,and caspase 1 protein expression.Enzyme linked immunosorbent assay(ELISA)was measured CE and ASM levels,while qRT-PCR assessed mRNA expression.The apoptotic rate was evaluated by flow cytometry(FCM).Results:Following FFAs incubation,significant increases in ASM and CE levels were observed in HepG2 and HL-7702 cells,accompanied by elevated expression of NLRP3,and caspase 1,and IL-1β.TNF-αtreatment further amplified these indicators.Ami demonstrated a reduction in lipid deposition,suppressed ASM/CE pathway activation,downregulated NLRP3 and caspase 1 expression,and improved apoptosis.Additionally,MCC950,a selective inhibitor of the NLRP3,mitigated NLRP3,caspase 1,and IL-1βexpression,alleviating lipid deposition and apoptosis in the NAFLD cell model.Conclusion:The ASM/CE-NLRP3 pathway in NAFLD cells promotes hepatocyte steatosis,inflammation,and cell damage.Ami emerges as a promising therapeutic agent by inhibiting the ASM/CE-NLRP3 pathway,underscoring its potential as a key target for NAFLD treatment.展开更多
头相关传输函数(Head Related Transfer Function, HRTF)在空间音频渲染中具有关键作用,能够显著提升个体的听觉体验。然而,要实现最佳的听觉效果,HRTF必须与受试者的解剖特征相符。为了达到这一目标,采用了一种基于人体测量特征的方法...头相关传输函数(Head Related Transfer Function, HRTF)在空间音频渲染中具有关键作用,能够显著提升个体的听觉体验。然而,要实现最佳的听觉效果,HRTF必须与受试者的解剖特征相符。为了达到这一目标,采用了一种基于人体测量特征的方法来估算个体化HRTF,特别地,扩展了耳廓的测量参数,并考虑了常被忽略的耳廓腔室对HRTF的影响。通过主动形状模型(Active Shape Models, ASM),自动从特定耳廓的标记点提取耳廓测量参数。接着,使用轻量级梯度提升机(Light Gradient Boosting Machine, LightGBM)模型,根据提取的耳廓测量参数及头部测量参数,预测个体在中垂面上的HRTF幅度。评估结果显示,所提取的耳廓特征能够显著提升HRTF个性化的客观性能指标。展开更多
基金国家自然科学基金,国家高技术研究发展计划(863计划),国家重点基础研究发展计划(973计划),the Foundation for Extraordinary Young Researchers under
文摘A new semantic model in Abstract State Model (ASM) for authentication protocols is presented. It highlights the Woo-Lam's ideas for authentication, which is the strongest one in Lowe's definition hierarchy for entity authentication. Apart from the flexible and natural features in forming and analyzing protocols inherited from ASM, the model defines both authentication and secrecy properties explicitly in first order sentences as invariants. The process of proving security properties with respect to an authentication protocol blends the correctness and secrecy properties together to avoid the potential flaws which may happen when treated separately. The security of revised Helsinki protocol is shown as a case study. The new model is different from the previous ones in ASMs.
基金supported by the Initial Scientific Research Fund of the Talents Introduced in Nanjing Lishui People’s Hospital(Project 2021YJ02).
文摘Background:Nonalcoholic fatty liver disease(NAFLD)is a global health concern with the acid sphingomyelinase(ASM)/ceramide(CE)pathway and the NOD-like receptor family,pyrin domain-containing protein 3(NLRP3)inflammasome identified as pivotal players in lipid disorders and inflammation.This study explores the interaction mechanism between the ASM/CE pathway and NLRP3 in NAFLD cell models,aiming to understand the impact of amitriptyline(Ami),an ASM inhibitor,on lipid deposition and hepatocyte injury by regulating the ASM/CE-NLRP3 pathway.Methods:HepG2 and HL-7702 cells were exposed to free fatty acids(FFAs)to establish the NAFLD model.The cells were divided into 5 groups:control group,model group,Ami group,tumor necrosis factoralpha(TNF-α)group,and Ami+TNF-αgroup.Intracellular lipid droplets were visualized using Oil Red O staining,and Western blot analysis quantified ASM,NLRP3,and caspase 1 protein expression.Enzyme linked immunosorbent assay(ELISA)was measured CE and ASM levels,while qRT-PCR assessed mRNA expression.The apoptotic rate was evaluated by flow cytometry(FCM).Results:Following FFAs incubation,significant increases in ASM and CE levels were observed in HepG2 and HL-7702 cells,accompanied by elevated expression of NLRP3,and caspase 1,and IL-1β.TNF-αtreatment further amplified these indicators.Ami demonstrated a reduction in lipid deposition,suppressed ASM/CE pathway activation,downregulated NLRP3 and caspase 1 expression,and improved apoptosis.Additionally,MCC950,a selective inhibitor of the NLRP3,mitigated NLRP3,caspase 1,and IL-1βexpression,alleviating lipid deposition and apoptosis in the NAFLD cell model.Conclusion:The ASM/CE-NLRP3 pathway in NAFLD cells promotes hepatocyte steatosis,inflammation,and cell damage.Ami emerges as a promising therapeutic agent by inhibiting the ASM/CE-NLRP3 pathway,underscoring its potential as a key target for NAFLD treatment.