Objective To probe into whether an acupoint-like and meridian-like structure was existed in platyfish. Methods Adult platyfish was put in 30 μM 4-Di-2-ASP water solution for 3 h, then, in pipe water for 20min, afterw...Objective To probe into whether an acupoint-like and meridian-like structure was existed in platyfish. Methods Adult platyfish was put in 30 μM 4-Di-2-ASP water solution for 3 h, then, in pipe water for 20min, afterward, the fish was anesthetized in 10% aether water solution, and the fluorescent labeling was observed under fluorescent microscope with B-3A combination filter. Results The labels observed under the microscope were in round bright dot, a majority of dots were distributed separately and a part of them was in cluster （2- 5 dots/cluster） on various parts of the body in regular arrangement. The labels on the head were circularly distributed around the eye and two arches were formed posterior to the eye and in the inferior 3/4 quadrant. These two arches joined one arch in the anterior superior 1/4 quadrant. On the fish trunk, it was observed that the labels were distributed from the back to the abdomen along the longitudinal axis of the trunk, forming 6 lines, located on No. 2, 4, 6, 7, 8 and 9 scale rows successively on the dorsal part of fish. Each line was composed of 7 to 22 label clusters and 1 -5 labels were counted in each cluster. The labels were arranged as 3-4 lines on the tail. Conclusion 1） Acupoint-like and meridian-like structure was existed in platyfish. 2）The skin sensory organs of animal were not distributed evenly all over the body. Instead, a number of sensory organs were put together in cluster and a number of them were in linear distribution regularly along the long axis of the trunk, which was similar to the distribution of traditional meridians and acupoints.展开更多
目的:基于国内外针刺疗法治疗变应性鼻炎(AR)的动物实验及临床研究文献,总结针刺治疗AR的研究特点,为优化针刺研究提供依据和思路。方法:检索国家知识基础设施数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)、中国...目的:基于国内外针刺疗法治疗变应性鼻炎(AR)的动物实验及临床研究文献,总结针刺治疗AR的研究特点,为优化针刺研究提供依据和思路。方法:检索国家知识基础设施数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)、中国生物医学文献数据库(CBM)、PubMed、Embase、Web of Science共7个数据库2013年1月至2023年8月发表的文献,收集主要干预方式为针刺,研究对象为AR模型动物或AR患者,且结局指标包含AR发病机制相关的生物学标志物的文献,对纳入文献的研究类型、干预措施、腧穴分布、高频腧穴、腧穴归经、穴位机制相关性进行汇总与分析,并评价纳入文献的质量。结果:共纳入35篇文献,其中临床试验25篇,动物研究10篇。所用腧穴共34个,频次≥5的高频腧穴共12个。生物标志物共31个,以炎症机制与免疫机制为主。分析的高频腧穴多用于研究免疫指标免疫球蛋白E以及炎症介质白细胞介素-4。纳入文献总体质量较低。结论:迎香穴使用频次较多,针刺治疗AR的操作方法及选穴尚无统一标准;目前免疫-炎症调节机制是针刺AR的主要研究热点,但研究深度较浅;需加强理论基础与动物研究、临床试验三者的结合,未来仍需大量标准化的研究加以探索和验证。展开更多
文摘Objective To probe into whether an acupoint-like and meridian-like structure was existed in platyfish. Methods Adult platyfish was put in 30 μM 4-Di-2-ASP water solution for 3 h, then, in pipe water for 20min, afterward, the fish was anesthetized in 10% aether water solution, and the fluorescent labeling was observed under fluorescent microscope with B-3A combination filter. Results The labels observed under the microscope were in round bright dot, a majority of dots were distributed separately and a part of them was in cluster （2- 5 dots/cluster） on various parts of the body in regular arrangement. The labels on the head were circularly distributed around the eye and two arches were formed posterior to the eye and in the inferior 3/4 quadrant. These two arches joined one arch in the anterior superior 1/4 quadrant. On the fish trunk, it was observed that the labels were distributed from the back to the abdomen along the longitudinal axis of the trunk, forming 6 lines, located on No. 2, 4, 6, 7, 8 and 9 scale rows successively on the dorsal part of fish. Each line was composed of 7 to 22 label clusters and 1 -5 labels were counted in each cluster. The labels were arranged as 3-4 lines on the tail. Conclusion 1） Acupoint-like and meridian-like structure was existed in platyfish. 2）The skin sensory organs of animal were not distributed evenly all over the body. Instead, a number of sensory organs were put together in cluster and a number of them were in linear distribution regularly along the long axis of the trunk, which was similar to the distribution of traditional meridians and acupoints.
文摘目的:基于国内外针刺疗法治疗变应性鼻炎(AR)的动物实验及临床研究文献,总结针刺治疗AR的研究特点,为优化针刺研究提供依据和思路。方法:检索国家知识基础设施数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)、中国生物医学文献数据库(CBM)、PubMed、Embase、Web of Science共7个数据库2013年1月至2023年8月发表的文献,收集主要干预方式为针刺,研究对象为AR模型动物或AR患者,且结局指标包含AR发病机制相关的生物学标志物的文献,对纳入文献的研究类型、干预措施、腧穴分布、高频腧穴、腧穴归经、穴位机制相关性进行汇总与分析,并评价纳入文献的质量。结果:共纳入35篇文献,其中临床试验25篇,动物研究10篇。所用腧穴共34个,频次≥5的高频腧穴共12个。生物标志物共31个,以炎症机制与免疫机制为主。分析的高频腧穴多用于研究免疫指标免疫球蛋白E以及炎症介质白细胞介素-4。纳入文献总体质量较低。结论:迎香穴使用频次较多,针刺治疗AR的操作方法及选穴尚无统一标准;目前免疫-炎症调节机制是针刺AR的主要研究热点,但研究深度较浅;需加强理论基础与动物研究、临床试验三者的结合,未来仍需大量标准化的研究加以探索和验证。
