Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces...Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.展开更多
The requirement for dietary n-3 HUFA (highly unsaturated fatty acid ) for growth and survival of black seabream (Sparus macrocephalus) larvae was tested using Artemia with various levels of n-3 HUFA. Four tueatments w...The requirement for dietary n-3 HUFA (highly unsaturated fatty acid ) for growth and survival of black seabream (Sparus macrocephalus) larvae was tested using Artemia with various levels of n-3 HUFA. Four tueatments with Artemia differing in theirn-3 HUFA were prepared by feeding them various oil emulsions .The results indicated that dietary n-3 HUFA significantly influences fish n-3 HUFA levels and are necessary for good growth and survival of black seabream larvae . The proper value of n-3 HUFA level and DHA, EPA level in Artemia should be 4.273% and 0.873% (wet weight) for good growth and survival in black seabream larvae respectively.展开更多
Colour production in fish is due mostly to food. In conditions of captivity, the type of food is restricted, while various types of food are used in aquaculture, from processed dry food to small aquatic animals. In th...Colour production in fish is due mostly to food. In conditions of captivity, the type of food is restricted, while various types of food are used in aquaculture, from processed dry food to small aquatic animals. In this study, we used nauplii of Artemia franciscana, “water fleas” Moina wierzejski, micro-worm Panagrellus redivivus, and commercial flakes. We used Poecilia reticulata, which is one of the most traded fish in ornamental aquaculture and hypothesise that if the live food influences the coloration and growth of P. reticulata, there must be differences in the intensity of colour pattern and growth rate in fish fed with different living animals. Consistent with our prediction, females and males of P. reticulata were more colourful when they were fed on A. franciscana, P. redivivus and M. wierzejski than when they were fed commercial flakes. Females and males of P. reticulata fed with A. franciscana grew in less time than fish fed with P. redivivus, M. wierzejski, or commercial flakes. We conclude that live food is an excellent way to affect growth and coloration in fish.展开更多
基金Supported by the Yangtze Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT_17R81)the Technology Support Project of Tianjin(No.16YFZCNC00810)
文摘Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.
文摘The requirement for dietary n-3 HUFA (highly unsaturated fatty acid ) for growth and survival of black seabream (Sparus macrocephalus) larvae was tested using Artemia with various levels of n-3 HUFA. Four tueatments with Artemia differing in theirn-3 HUFA were prepared by feeding them various oil emulsions .The results indicated that dietary n-3 HUFA significantly influences fish n-3 HUFA levels and are necessary for good growth and survival of black seabream larvae . The proper value of n-3 HUFA level and DHA, EPA level in Artemia should be 4.273% and 0.873% (wet weight) for good growth and survival in black seabream larvae respectively.
文摘Colour production in fish is due mostly to food. In conditions of captivity, the type of food is restricted, while various types of food are used in aquaculture, from processed dry food to small aquatic animals. In this study, we used nauplii of Artemia franciscana, “water fleas” Moina wierzejski, micro-worm Panagrellus redivivus, and commercial flakes. We used Poecilia reticulata, which is one of the most traded fish in ornamental aquaculture and hypothesise that if the live food influences the coloration and growth of P. reticulata, there must be differences in the intensity of colour pattern and growth rate in fish fed with different living animals. Consistent with our prediction, females and males of P. reticulata were more colourful when they were fed on A. franciscana, P. redivivus and M. wierzejski than when they were fed commercial flakes. Females and males of P. reticulata fed with A. franciscana grew in less time than fish fed with P. redivivus, M. wierzejski, or commercial flakes. We conclude that live food is an excellent way to affect growth and coloration in fish.
