This study aimed to explore simple culture techniques for symbiotic germination-promoting fungi and Armillariella mellea in artificial planting of Gastrodia elata, i.e., how to use a corner of ordinary house as inocul...This study aimed to explore simple culture techniques for symbiotic germination-promoting fungi and Armillariella mellea in artificial planting of Gastrodia elata, i.e., how to use a corner of ordinary house as inoculation room and cultivating site, how to use a pressure cooker for cooking food as sterilization tool, and how to use ordinary household heating as heating measures.展开更多
By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were ...By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electricfield.The results showed that compared with the control plants,low-intensity-voltage(1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.展开更多
In a commercialized, fully artificial plant factory, artificial luminaire is arranged in a unified way using a general illumination theory, an actual measurement, or an empirical methodology. However, with these metho...In a commercialized, fully artificial plant factory, artificial luminaire is arranged in a unified way using a general illumination theory, an actual measurement, or an empirical methodology. However, with these methods, lightings are implemented without considering specific optical characteristics of lighting or material characteristics of each component that constructs a cultivation system, resulting in an amount of light that becomes irregular. The amount of lighting is closely related with the growth and quality of crops, and the deviation between points where cultivated crops are located causes quality difference in the produced crops, thus impairing the economic feasibility of a plant factory. In this regard, a simulation to figure out an optimum lighting layout was performed. Arrangements based on the spectrum distribution of light source and reflector materials were implemented to ascertain the distance between lighting and height of lighting and gather information in the pre-treatment process to improve the uniformity of light in the plant cultivation system. Improvement of around 15% in light uniformity is achieved compared with the existing system after the simulation is carried out. This result would reduce the deviation in crop growth to make uniform quality crop production possible.展开更多
The challenges and opportunities for developing sustainable plant factories with artificial lighting(PFALs)are discussed.After examining the production cost and productivity of existing PFALs in Japan,the possibility ...The challenges and opportunities for developing sustainable plant factories with artificial lighting(PFALs)are discussed.After examining the production cost and productivity of existing PFALs in Japan,the possibility of introducing a relatively new concept and methodology for considerably improving productivity are discussed in relation to environmental controllability and resource use efficiencies.The fundamental and potential characteristics of ideal or next-generation PFALs(n-PFALs)are then discussed with some suggestions for actualizing n-PFALs.Finally,perspectives of the n-PFALs and technologies to be integrated into the n-PFALs are presented in relation to the Sustainable Development Goals(SDGs)to be achieved by 2030.展开更多
The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was l...The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable,In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.展开更多
基金Supported by Key Natural Science Research Project of Sichuan Provincial Departmen of Education(12ZA102)~~
文摘This study aimed to explore simple culture techniques for symbiotic germination-promoting fungi and Armillariella mellea in artificial planting of Gastrodia elata, i.e., how to use a corner of ordinary house as inoculation room and cultivating site, how to use a pressure cooker for cooking food as sterilization tool, and how to use ordinary household heating as heating measures.
基金Supported by Natural Science Foundation of Shanghai(10ZR1400300 )Central University Special Foundation of Basic Research and Operating expenses+1 种基金Creative Group Foundation of the National Natural Science Foundation of China (50721006)Key Discipline construction Project of Shanghai (B604)~~
文摘By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electricfield.The results showed that compared with the control plants,low-intensity-voltage(1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.
基金financially supported by the Ministry of Education, Science, and Technology (MEST)the National Research Foundation of Korea (NRF) through the Human Resource Training Project for Regional Innovationsupported by the Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning (No.20114010203040) grant funded by the Korean government’s Ministry of Knowledge Economy
文摘In a commercialized, fully artificial plant factory, artificial luminaire is arranged in a unified way using a general illumination theory, an actual measurement, or an empirical methodology. However, with these methods, lightings are implemented without considering specific optical characteristics of lighting or material characteristics of each component that constructs a cultivation system, resulting in an amount of light that becomes irregular. The amount of lighting is closely related with the growth and quality of crops, and the deviation between points where cultivated crops are located causes quality difference in the produced crops, thus impairing the economic feasibility of a plant factory. In this regard, a simulation to figure out an optimum lighting layout was performed. Arrangements based on the spectrum distribution of light source and reflector materials were implemented to ascertain the distance between lighting and height of lighting and gather information in the pre-treatment process to improve the uniformity of light in the plant cultivation system. Improvement of around 15% in light uniformity is achieved compared with the existing system after the simulation is carried out. This result would reduce the deviation in crop growth to make uniform quality crop production possible.
文摘The challenges and opportunities for developing sustainable plant factories with artificial lighting(PFALs)are discussed.After examining the production cost and productivity of existing PFALs in Japan,the possibility of introducing a relatively new concept and methodology for considerably improving productivity are discussed in relation to environmental controllability and resource use efficiencies.The fundamental and potential characteristics of ideal or next-generation PFALs(n-PFALs)are then discussed with some suggestions for actualizing n-PFALs.Finally,perspectives of the n-PFALs and technologies to be integrated into the n-PFALs are presented in relation to the Sustainable Development Goals(SDGs)to be achieved by 2030.
基金supported by the Ministry of Education of China and Agriculture and Agri-Food Canada(MOE-AAFC) PhD student research program
文摘The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable,In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.
