Modifying the knots of a B-spline curve, the shape of the curve will be changed. In this paper, we present the effect of the symmetric alteration of four knots of the B-spline and the NURBS surfaces, i.e., symmetrical...Modifying the knots of a B-spline curve, the shape of the curve will be changed. In this paper, we present the effect of the symmetric alteration of four knots of the B-spline and the NURBS surfaces, i.e., symmetrical alteration of the knots of surface, the extended paths of points of the surface will converge to a point which should be expressed with several control points. This theory can be used in the constrained shape modification of B-spline and NURBS surfaces.展开更多
Routing algorithms capable of providing quality of service （QoS） will play an important role in future communication networks. For the trajectory-based routing （ TBR）, An effective method of en- coding trajectorie...Routing algorithms capable of providing quality of service （QoS） will play an important role in future communication networks. For the trajectory-based routing （ TBR）, An effective method of en- coding trajectories into packets is proposed. The method uses a B-spline curve, which provides a lot of flexibility. The simulation results show that the performance of the proposed algorithms is im- proved significantly compared with the existing algorithm.展开更多
Objective： Chronic lymphocytic leukemia （CLL） and mantle cell lymphoma （MCL） cells over-express a guanine exchange factor （GEF）, Rasgrf-1. This GEF increases active Ras as it catalyzes the removal of GDP from R...Objective： Chronic lymphocytic leukemia （CLL） and mantle cell lymphoma （MCL） cells over-express a guanine exchange factor （GEF）, Rasgrf-1. This GEF increases active Ras as it catalyzes the removal of GDP from Ras so that GTP can bind and activate Ras. This study aims to study the mechanism of action of Rasgrf-1 in B-cell malignancies. Methods： N-terminus truncated Rasgrf-1 variants have a higher GEF activity as compared to the full-length transcript therefore a MCL cell line with stable over-expression of truncated Rasgrf-1 was established. The B-cell receptor （BCR） and chemokine signaling pathways were compared in the Rasgrf-I over-expressing and a control transfected cell line. Results： Cells over-expressing truncated form of Rasgrf-1 have a higher proliferative rate as compared to control transfected cells. BCR was activated by lower concentrations of anti-IgM antibody in Rasgrf-1 over-expressing cells as compared to control cells indicating that these cells are more sensitive to BCR signaling. BCR signaling also phosphorylates Rasgrf-1 that further increases its GEF function and amplifies BCR signaling. This activation of Rasgrf-1 in over-expressing cells resulted in a higher expression of phospho-ERK, AKT, BTK and PKC-alpha as compared to control cells. Besides BCR, Rasgrf-1 over-expressing cells were also more sensitive to microenvironment stimuli as determined by resistance to apoptosis, chemotaxis and ERK pathway activation. Conclusions： This GEF protein sensitizes B-cells to BCR and chemokine mediated signaling and also upregulates a number of other signaling pathways which promotes growth and survival of these cells.展开更多
A new LDMOST structure, named B-LDMOST that has a buried layer under the drain is proposed. The buried layer is not connected to the drift region, so it can optimize the vertical field distribution and increase breakd...A new LDMOST structure, named B-LDMOST that has a buried layer under the drain is proposed. The buried layer is not connected to the drift region, so it can optimize the vertical field distribution and increase breakdown voltage. The analysis and the simulated results show that B-LDMOST can increase breakdown voltage, with almost negligible influence on the other parameters such as on-resistance, switching time, and so on.展开更多
A low-power and high-speed 16:1 MUX IC designed for Optical fiber communication based on TSMC 0.25 μm CMOS technology is represented. A tree-type architecture was utilized. The output data bit rate is 2.5 Gb/s at inp...A low-power and high-speed 16:1 MUX IC designed for Optical fiber communication based on TSMC 0.25 μm CMOS technology is represented. A tree-type architecture was utilized. The output data bit rate is 2.5 Gb/s at input clock rate of 1.25 GHz. The simulation results show that the output signal has peak-to-peak amplitude of 400 mV, the power dissipation is less than 200 mW and the power dissipation of core circuit is less than 20 mW at the 2.5 Gb/s standard bit rate and supply voltage of 2.5 V. The chip area is （1.8） mm2.展开更多
Objective: To investigate the expression of Tbx2 protein in pancreatic cancer tissues and its molecular regulation mechanism by Wnt/β-catenin signaling. Methods: 49 pancreatic cancer and 13 non-cancer tissue specimen...Objective: To investigate the expression of Tbx2 protein in pancreatic cancer tissues and its molecular regulation mechanism by Wnt/β-catenin signaling. Methods: 49 pancreatic cancer and 13 non-cancer tissue specimens were obtained and examined the expression of Tbx2, and the correlation between the expression of Tbx2 and clinicopathological parameters was analyzed. The immunohistochemistry, immunocytochemistry, RT-PCR and Western blot assay methods were used to detect the changes of expression levels of β-catenin and Tbx2. Results: Tbx2 was amplified in 34 of 49 pancreatic cancers, and in 13 non-cancer tissues, only one sample amplified. The further study demonstrated that Tbx2 had a significant positive correlation with tumor differentiation degree and clinical stage, but it did not relate to the sex, age and the disease region. Inhibi-tion of β-catenin degradation through the treatment of pancreatic cancer cells SW1990 with different concentrations of lithium chloride indicated that accumulation of β-catenin was sufficient to induce TBX2 expression. Conclusion: TBX2 gene plays an important role in the occurrence and development of pancreatic cancer and the accumulation of β-catenin contributes to the expression of TBX2. The Wnt/β-catenin signaling pathway participates the regulation of TBX2 in pancreatic cancer cells.展开更多
Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neurob...Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse.Based on the analysis of experimental results,it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry.This result showed that PPP had anti-aging effects on D-galactoseinduced aging mouse model.Moreover,we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3,8,and 9 proteins in the PPP-treated mouse hippocampus.Therefore,our results showed that PPP obviously improved neuroblast proliferation and differentiation,and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins,including Caspase 3,8,and 9,via BDNF/TrkB pathway.Our findings provided valuable evidence for its applications in the health and medicine sectors.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 60473130) and the National Basic Research Program (973) of China (No. G2004CB318000)
文摘Modifying the knots of a B-spline curve, the shape of the curve will be changed. In this paper, we present the effect of the symmetric alteration of four knots of the B-spline and the NURBS surfaces, i.e., symmetrical alteration of the knots of surface, the extended paths of points of the surface will converge to a point which should be expressed with several control points. This theory can be used in the constrained shape modification of B-spline and NURBS surfaces.
基金Supported by the National Natural Science Foundation of China （11171316）, and the Zhejiang Provincial Natural Science Foundation of China （No. Y6090472）.
文摘Routing algorithms capable of providing quality of service （QoS） will play an important role in future communication networks. For the trajectory-based routing （ TBR）, An effective method of en- coding trajectories into packets is proposed. The method uses a B-spline curve, which provides a lot of flexibility. The simulation results show that the performance of the proposed algorithms is im- proved significantly compared with the existing algorithm.
文摘Objective： Chronic lymphocytic leukemia （CLL） and mantle cell lymphoma （MCL） cells over-express a guanine exchange factor （GEF）, Rasgrf-1. This GEF increases active Ras as it catalyzes the removal of GDP from Ras so that GTP can bind and activate Ras. This study aims to study the mechanism of action of Rasgrf-1 in B-cell malignancies. Methods： N-terminus truncated Rasgrf-1 variants have a higher GEF activity as compared to the full-length transcript therefore a MCL cell line with stable over-expression of truncated Rasgrf-1 was established. The B-cell receptor （BCR） and chemokine signaling pathways were compared in the Rasgrf-I over-expressing and a control transfected cell line. Results： Cells over-expressing truncated form of Rasgrf-1 have a higher proliferative rate as compared to control transfected cells. BCR was activated by lower concentrations of anti-IgM antibody in Rasgrf-1 over-expressing cells as compared to control cells indicating that these cells are more sensitive to BCR signaling. BCR signaling also phosphorylates Rasgrf-1 that further increases its GEF function and amplifies BCR signaling. This activation of Rasgrf-1 in over-expressing cells resulted in a higher expression of phospho-ERK, AKT, BTK and PKC-alpha as compared to control cells. Besides BCR, Rasgrf-1 over-expressing cells were also more sensitive to microenvironment stimuli as determined by resistance to apoptosis, chemotaxis and ERK pathway activation. Conclusions： This GEF protein sensitizes B-cells to BCR and chemokine mediated signaling and also upregulates a number of other signaling pathways which promotes growth and survival of these cells.
基金Supported by the National Natural Science Foundation of China(No.69776041)
文摘A new LDMOST structure, named B-LDMOST that has a buried layer under the drain is proposed. The buried layer is not connected to the drift region, so it can optimize the vertical field distribution and increase breakdown voltage. The analysis and the simulated results show that B-LDMOST can increase breakdown voltage, with almost negligible influence on the other parameters such as on-resistance, switching time, and so on.
文摘A low-power and high-speed 16:1 MUX IC designed for Optical fiber communication based on TSMC 0.25 μm CMOS technology is represented. A tree-type architecture was utilized. The output data bit rate is 2.5 Gb/s at input clock rate of 1.25 GHz. The simulation results show that the output signal has peak-to-peak amplitude of 400 mV, the power dissipation is less than 200 mW and the power dissipation of core circuit is less than 20 mW at the 2.5 Gb/s standard bit rate and supply voltage of 2.5 V. The chip area is （1.8） mm2.
文摘Objective: To investigate the expression of Tbx2 protein in pancreatic cancer tissues and its molecular regulation mechanism by Wnt/β-catenin signaling. Methods: 49 pancreatic cancer and 13 non-cancer tissue specimens were obtained and examined the expression of Tbx2, and the correlation between the expression of Tbx2 and clinicopathological parameters was analyzed. The immunohistochemistry, immunocytochemistry, RT-PCR and Western blot assay methods were used to detect the changes of expression levels of β-catenin and Tbx2. Results: Tbx2 was amplified in 34 of 49 pancreatic cancers, and in 13 non-cancer tissues, only one sample amplified. The further study demonstrated that Tbx2 had a significant positive correlation with tumor differentiation degree and clinical stage, but it did not relate to the sex, age and the disease region. Inhibi-tion of β-catenin degradation through the treatment of pancreatic cancer cells SW1990 with different concentrations of lithium chloride indicated that accumulation of β-catenin was sufficient to induce TBX2 expression. Conclusion: TBX2 gene plays an important role in the occurrence and development of pancreatic cancer and the accumulation of β-catenin contributes to the expression of TBX2. The Wnt/β-catenin signaling pathway participates the regulation of TBX2 in pancreatic cancer cells.
基金The University Natural Science Research major Project of Jiangsu Province(Grant No.16KJA310006)
文摘Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse.Based on the analysis of experimental results,it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry.This result showed that PPP had anti-aging effects on D-galactoseinduced aging mouse model.Moreover,we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3,8,and 9 proteins in the PPP-treated mouse hippocampus.Therefore,our results showed that PPP obviously improved neuroblast proliferation and differentiation,and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins,including Caspase 3,8,and 9,via BDNF/TrkB pathway.Our findings provided valuable evidence for its applications in the health and medicine sectors.
