Background:Buyang Huanwu decoction(BHD)is a traditional Chinese medicine herbal formula used for treating hypertension,particularly in the later stages of hypertension when it is associated with intracerebral hemorrha...Background:Buyang Huanwu decoction(BHD)is a traditional Chinese medicine herbal formula used for treating hypertension,particularly in the later stages of hypertension when it is associated with intracerebral hemorrhage.This study aims to investigate the treatment mechanism of BHD to provide a basis for its clinical application in hypertension treatment.Methods:Network pharmacology analysis and cell culture experiments were performed to explore the potential proteins and mechanisms of action of BHD against hypertension.Bioactive compounds related to BHD were screened,and relevant targets associated with hypertension and BHD were retrieved.Molecular docking technology was used to identify the effective signaling pathway based on the Kyoto Encyclopedia of Genes and Genomes and protein-protein interaction network cores.Lastly,the effects and mechanisms of BHD on salt-sensitive hypertensive endothelial cells were investigated.Results:Ninety-three potential therapeutic targets for BHD and salt-sensitive hypertension were found to be closely associated with the PI3K/Akt/eNOS signaling pathway and oxidative stress.Cell experiments further indicated the pivotal role of endothelial cells in hypertension,and validation analysis showed that BHD significantly preserved cell morphology,suppressed oxidative stress reactions,activated the PI3K/Akt/eNOS signaling pathways,preserved normal endothelial cell function,and reduced cell apoptosis.Conclusion:BHD effectively activates the PI3K/Akt/VEGF signaling pathway,attenuates oxidative stress-induced injury in endothelial cells exposed to high salt levels,and mitigates apoptosis,supporting the use of traditional Chinese medicine BHD in the treatment of salt-sensitive hypertension.展开更多
The aim of the paper is to explore whether AMPK/U1K1 signaling pathway is involved in regulating autophagy and alleviating cerebral ischemia/reperfusion(I/R)injury.On this basis,study the regulatory effect of Buyang H...The aim of the paper is to explore whether AMPK/U1K1 signaling pathway is involved in regulating autophagy and alleviating cerebral ischemia/reperfusion(I/R)injury.On this basis,study the regulatory effect of Buyang Huanwu Decoction(BHD)on AMPK/U1K1 signaling pathway,aiming to clarify the molecular mechanism of BHD in alleviating cerebral I/R injury and the protective effect of BHD containing serum(BHDS)on OGD/R neurons.The research group adopted the following methods:Neurons cultured for 7 days in vitro were randomly divided into normal control group,model group(OGD/R),AMPK activator(AICAR)group(3 mmol/L),Buyang Huanwu Decoction medicated serum(BHDS)group(3%),Buyang Huanwu Decoction medicated serum+AMPK activator(BHDS+AICAR)group.The protein expressions of p-AMPK,AMPK,p-UlKl,U1K1,Beclin-1 and LC3B in neurons were detected by Western blot,the fluorescence intensity and apoptosis rate of neuron autophagosome CYTO-ID staining were detected by flow cytometry,the fluorescence intensity and morphological changes of neuron LC3B were detected by immunofluorescence double labeling method,the morphological changes of neurons were observed by inverted microscope,and the survival rate of neurons was detected by CCK-8 method.Neuron OGD/R model and the optimal concentration of BHDS.The neurons in the model group were subjected to hypoxia and glucose deprivation for 1 h,2 h,3 h,4 h and reoxygenation and glucose deprivation for 24 h.The survival rate of neurons and the release rate of 1DH to determine whether the model was successfully established.And the results show that BHDS group,the survival rate of OGD/R neurons was significantly increased,and the apoptosis rate was significantly decreased.Among them,3%BHDS intervention at the same time of hypoxia glucose deprivation for 3 h and reoxygenation glucose for 24 h was the best condition.In BHDS group,the levels of p-AMPK/AMPK,p-UlKl/UlKl,beclin-1,CYTO-ID and LC3B fluorescence intensity,and the transformation from LC3B-I to LC3B-II of OGD/R neurons were significantly decreased.So we can draw a conclusion that BHD can inhibit autophagy and alleviate cerebral I/R injury through AMPK/U1K1 signaling pathway.展开更多
Background: Approximately 830 women die from pregnancy-related conditions daily with 99% of these maternal deaths occurring in low resource countries. Primary postpartum haemorrhage (PPH) accounts for 25.7% of materna...Background: Approximately 830 women die from pregnancy-related conditions daily with 99% of these maternal deaths occurring in low resource countries. Primary postpartum haemorrhage (PPH) accounts for 25.7% of maternal deaths in Africa. In Cameroon, postpartum hemorrhage remains the leading cause of maternal death, with little information on the primigravidae population compared to multigravida or multiparity. Objective: The aim of this study was to assess the factors associated with postpartum haemorrhage amongst primigravidae women giving birth in two hospitals within the Bamenda Health District. Methods: This study was a multicentric, non-randomized cross-sectional descriptive and analytic study. Of the 221 women interviewed regarding their willingness to participate in the study, 197 consented. Quantification of blood loss was done by visual estimate and with the assistance of the pathfinder international wall chart for visual estimation of blood loss. Data was collected using a structured questionnaire and analyzed using SPSS version 23. A P-value of <0.05 used to determine association between variables was considered statistically significant. Results: The prevalence of postpartum hemorrhage in primigravidae was 7.1%. The risk factors of postpartum hemorrhage were: induction of labour (P-value < 0.01), duration of labour (P-value < 0.01), augmentation (P-value < 0.05), mode of delivery (P-value < 0.01), and macrosomia (P-value < 0.01). The main causes of PPH were uterine atony and obstetrical lacerations (P value < 0.01). Management was mostly by the use of non-pharmacological and pharmacological measures. The main adverse outcomes were shock and severe anaemia, with one case of nearmiss recorded. No maternal death was recorded. Conclusion: The prevalence of postpartum hemorrhage amongst primigravidae was high. The main causes of post-partum hemorrhage were uterine atony and obstetric lacerations. PPH was associated with uterine hypotonia, retained products and genital lacerations. No maternal mortality was recorded. These findings highlight the pressing need for good quality em ergency obstetric care and the availability of more accurate techniques of postpartum blood loss measurement. Secondly, hospitals need blood banks to manage patients with severe hemorrhage.展开更多
基金the Liaoning Provincial Science and Technology Plan Project(2023-MSLH-178)the Project Fund of Liaoning Provincial Department of Education(LJKMZ20221315,L202025)+1 种基金the Special Fund of Liaoning Provincial Department of Science and Technology for Central Guidance of Local Science and Technology Development(2018416016)the Project Fund of Liaoning Provincial Department of Human Resources and Social Security"Millions of Talents Program"(2020921097).
文摘Background:Buyang Huanwu decoction(BHD)is a traditional Chinese medicine herbal formula used for treating hypertension,particularly in the later stages of hypertension when it is associated with intracerebral hemorrhage.This study aims to investigate the treatment mechanism of BHD to provide a basis for its clinical application in hypertension treatment.Methods:Network pharmacology analysis and cell culture experiments were performed to explore the potential proteins and mechanisms of action of BHD against hypertension.Bioactive compounds related to BHD were screened,and relevant targets associated with hypertension and BHD were retrieved.Molecular docking technology was used to identify the effective signaling pathway based on the Kyoto Encyclopedia of Genes and Genomes and protein-protein interaction network cores.Lastly,the effects and mechanisms of BHD on salt-sensitive hypertensive endothelial cells were investigated.Results:Ninety-three potential therapeutic targets for BHD and salt-sensitive hypertension were found to be closely associated with the PI3K/Akt/eNOS signaling pathway and oxidative stress.Cell experiments further indicated the pivotal role of endothelial cells in hypertension,and validation analysis showed that BHD significantly preserved cell morphology,suppressed oxidative stress reactions,activated the PI3K/Akt/eNOS signaling pathways,preserved normal endothelial cell function,and reduced cell apoptosis.Conclusion:BHD effectively activates the PI3K/Akt/VEGF signaling pathway,attenuates oxidative stress-induced injury in endothelial cells exposed to high salt levels,and mitigates apoptosis,supporting the use of traditional Chinese medicine BHD in the treatment of salt-sensitive hypertension.
基金the National Natural Science Foundation of China(81873180)the general program of the Natural Science Foundation of Hebei Province(H2019423074)+1 种基金the key program of Hebei University of Traditional Chinese Medicine(KTZ2019018)the central guiding local science and technology development fund(206Z7706G).
文摘The aim of the paper is to explore whether AMPK/U1K1 signaling pathway is involved in regulating autophagy and alleviating cerebral ischemia/reperfusion(I/R)injury.On this basis,study the regulatory effect of Buyang Huanwu Decoction(BHD)on AMPK/U1K1 signaling pathway,aiming to clarify the molecular mechanism of BHD in alleviating cerebral I/R injury and the protective effect of BHD containing serum(BHDS)on OGD/R neurons.The research group adopted the following methods:Neurons cultured for 7 days in vitro were randomly divided into normal control group,model group(OGD/R),AMPK activator(AICAR)group(3 mmol/L),Buyang Huanwu Decoction medicated serum(BHDS)group(3%),Buyang Huanwu Decoction medicated serum+AMPK activator(BHDS+AICAR)group.The protein expressions of p-AMPK,AMPK,p-UlKl,U1K1,Beclin-1 and LC3B in neurons were detected by Western blot,the fluorescence intensity and apoptosis rate of neuron autophagosome CYTO-ID staining were detected by flow cytometry,the fluorescence intensity and morphological changes of neuron LC3B were detected by immunofluorescence double labeling method,the morphological changes of neurons were observed by inverted microscope,and the survival rate of neurons was detected by CCK-8 method.Neuron OGD/R model and the optimal concentration of BHDS.The neurons in the model group were subjected to hypoxia and glucose deprivation for 1 h,2 h,3 h,4 h and reoxygenation and glucose deprivation for 24 h.The survival rate of neurons and the release rate of 1DH to determine whether the model was successfully established.And the results show that BHDS group,the survival rate of OGD/R neurons was significantly increased,and the apoptosis rate was significantly decreased.Among them,3%BHDS intervention at the same time of hypoxia glucose deprivation for 3 h and reoxygenation glucose for 24 h was the best condition.In BHDS group,the levels of p-AMPK/AMPK,p-UlKl/UlKl,beclin-1,CYTO-ID and LC3B fluorescence intensity,and the transformation from LC3B-I to LC3B-II of OGD/R neurons were significantly decreased.So we can draw a conclusion that BHD can inhibit autophagy and alleviate cerebral I/R injury through AMPK/U1K1 signaling pathway.
文摘Background: Approximately 830 women die from pregnancy-related conditions daily with 99% of these maternal deaths occurring in low resource countries. Primary postpartum haemorrhage (PPH) accounts for 25.7% of maternal deaths in Africa. In Cameroon, postpartum hemorrhage remains the leading cause of maternal death, with little information on the primigravidae population compared to multigravida or multiparity. Objective: The aim of this study was to assess the factors associated with postpartum haemorrhage amongst primigravidae women giving birth in two hospitals within the Bamenda Health District. Methods: This study was a multicentric, non-randomized cross-sectional descriptive and analytic study. Of the 221 women interviewed regarding their willingness to participate in the study, 197 consented. Quantification of blood loss was done by visual estimate and with the assistance of the pathfinder international wall chart for visual estimation of blood loss. Data was collected using a structured questionnaire and analyzed using SPSS version 23. A P-value of <0.05 used to determine association between variables was considered statistically significant. Results: The prevalence of postpartum hemorrhage in primigravidae was 7.1%. The risk factors of postpartum hemorrhage were: induction of labour (P-value < 0.01), duration of labour (P-value < 0.01), augmentation (P-value < 0.05), mode of delivery (P-value < 0.01), and macrosomia (P-value < 0.01). The main causes of PPH were uterine atony and obstetrical lacerations (P value < 0.01). Management was mostly by the use of non-pharmacological and pharmacological measures. The main adverse outcomes were shock and severe anaemia, with one case of nearmiss recorded. No maternal death was recorded. Conclusion: The prevalence of postpartum hemorrhage amongst primigravidae was high. The main causes of post-partum hemorrhage were uterine atony and obstetric lacerations. PPH was associated with uterine hypotonia, retained products and genital lacerations. No maternal mortality was recorded. These findings highlight the pressing need for good quality em ergency obstetric care and the availability of more accurate techniques of postpartum blood loss measurement. Secondly, hospitals need blood banks to manage patients with severe hemorrhage.
